BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,831	T→C	T262A (ACT→GCT)	yhfX ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,831	0	T	C	99.2%	1224.8 / ‑3.3	381	T262A (ACT→GCT)	yhfX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (186/192); total (187/194)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 391 total aligned reads displayed.

AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGT  >  NC_000913/3507741‑3507920

aGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGAGc                                                                                         >  1:5255045/1‑93 (MQ=255)

  cTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGccc                                                                                       <  2:8151877/93‑1 (MQ=255)

    gTCGCGACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGt                                                                                     <  2:5491816/93‑1 (MQ=255)

     tCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTc                                                                                    <  1:3632543/93‑1 (MQ=255)

     tCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTc                                                                                    >  2:3353042/1‑93 (MQ=255)

      cGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCa                                                                                   <  2:9189123/93‑1 (MQ=255)

          aCGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAgcgc                                                                               >  2:4254862/1‑93 (MQ=255)

          aCGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAgcgc                                                                               <  1:682078/93‑1 (MQ=255)

           cGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCa                                                                              <  1:8599435/93‑1 (MQ=255)

           cGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCa                                                                              >  1:7391413/1‑93 (MQ=255)

            ggAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCAt                                                                             <  2:612225/93‑1 (MQ=255)

              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                           <  1:5918841/93‑1 (MQ=255)

              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                           <  2:2029909/93‑1 (MQ=255)

              aaaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGa                                                                           <  1:1072711/93‑1 (MQ=255)

               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                          >  1:279028/1‑93 (MQ=255)

               aaTGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAc                                                                          <  2:5602161/93‑1 (MQ=255)

                atgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGTGCATGAcc                                                                         <  2:2826531/93‑1 (MQ=255)

                atgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGAcc                                                                         <  2:8030751/93‑1 (MQ=255)

                 tgatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCg                                                                        >  1:4600757/1‑93 (MQ=255)

                  gatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCgg                                                                       >  1:5490881/1‑93 (MQ=255)

                  gatgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGAATGACCgg                                                                       >  1:556171/1‑93 (MQ=255)

                   atgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCggg                                                                      <  1:9554114/93‑1 (MQ=255)

                    tgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGt                                                                     <  2:5293452/93‑1 (MQ=255)

                    tgGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGt                                                                     <  2:488944/93‑1 (MQ=255)

                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    <  1:6063992/93‑1 (MQ=255)

                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    <  2:4220427/93‑1 (MQ=255)

                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    >  1:672928/1‑93 (MQ=255)

                     gggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGtt                                                                    <  2:9581923/93‑1 (MQ=255)

                      ggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTc                                                                   >  1:2275553/1‑93 (MQ=255)

                      ggAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTc                                                                   <  1:7143323/93‑1 (MQ=255)

                       gagaTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCg                                                                  <  2:5754659/93‑1 (MQ=255)

                       gagaTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCg                                                                  <  1:6004341/93‑1 (MQ=255)

                        agaTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCgg                                                                 <  1:8046077/93‑1 (MQ=255)

                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                               <  2:3284256/93‑1 (MQ=255)

                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                               >  2:7535517/1‑93 (MQ=255)

                          aTTTCACTTAACCAGAGCATCGCGATACGTTCAAGCTGAACGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCa                                                               <  2:8949484/93‑1 (MQ=255)

                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                              >  2:2449366/1‑93 (MQ=255)

                           tttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCAt                                                              >  2:9759856/1‑93 (MQ=255)

                            ttCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATg                                                             >  1:9048288/1‑93 (MQ=255)

                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                            <  2:7653092/93‑1 (MQ=255)

                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                            >  1:8830660/1‑93 (MQ=255)

                             tCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGa                                                            >  2:1414226/1‑93 (MQ=255)

                               aCTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGt                                                          >  1:1716208/1‑93 (MQ=255)

                               aCTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGt                                                          >  1:6200529/1‑93 (MQ=255)

                                cTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTc                                                         <  1:5314822/93‑1 (MQ=255)

                                     ccAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACAcc                                                    <  2:4620730/93‑1 (MQ=255)

                                     ccAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACAcc                                                    <  2:1033823/93‑1 (MQ=255)

                                       agagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGt                                                  >  2:1772287/1‑93 (MQ=255)

                                       agagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGt                                                  >  2:5893552/1‑93 (MQ=255)

                                        gagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTa                                                 <  1:2612479/93‑1 (MQ=255)

                                        gagCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTa                                                 <  2:125985/93‑1 (MQ=255)

                                          gCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAtt                                               <  2:3876831/93‑1 (MQ=255)

                                          gCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAtt                                               >  1:8472941/1‑93 (MQ=255)

                                            aTCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgc                                             >  2:9329715/1‑93 (MQ=255)

                                             tCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTgcg                                            >  1:9134966/1‑93 (MQ=255)

                                             tCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTAGTgcg                                            >  2:7128136/1‑93 (MQ=255)

                                               gcgATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGAATGAGTCACACCGTATTGCGcc                                          <  1:1838062/93‑1 (MQ=255)

                                                 gATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAg                                        <  1:9722909/93‑1 (MQ=255)

                                                 gATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAg                                        >  2:6355439/1‑93 (MQ=255)

                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                       >  1:3389383/1‑93 (MQ=255)

                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                       >  1:2712639/1‑93 (MQ=255)

                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGt                                       >  1:2061728/1‑93 (MQ=255)

                                                  aTACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGACACACCGAATTGCGCCAGt                                       >  1:8641819/1‑93 (MQ=255)

                                                   tACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTa                                      <  2:8809310/93‑1 (MQ=255)

                                                   tACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTa                                      <  2:159500/93‑1 (MQ=255)

                                                    aCGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTaa                                     <  2:7936282/93‑1 (MQ=255)

                                                    aCGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTaa                                     <  2:5581757/93‑1 (MQ=255)

                                                      gTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACTCCGTATTGCGCCAGTAATg                                   >  2:8684136/1‑93 (MQ=255)

                                                      gTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTACTg                                   >  2:8400646/1‑93 (MQ=255)

                                                       ttCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATgg                                  <  1:9857808/93‑1 (MQ=255)

                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 >  2:1096355/1‑93 (MQ=255)

                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  1:6978470/93‑1 (MQ=255)

                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  2:2273955/93‑1 (MQ=255)

                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  1:7691913/93‑1 (MQ=255)

                                                        tCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGc                                 <  1:727667/93‑1 (MQ=255)

                                                          aGGCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAg                               >  2:5986003/1‑93 (MQ=255)

                                                           ggCTGATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGc                              <  1:1331014/93‑1 (MQ=255)

                                                               gATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAg                          <  1:4374786/93‑1 (MQ=255)

                                                               gATCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAg                          <  2:1680413/93‑1 (MQ=255)

                                                                aTCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGt                         <  1:4097963/93‑1 (MQ=255)

                                                                 tCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTg                        <  2:7329277/93‑1 (MQ=255)

                                                                 tCGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTg                        >  2:661289/1‑93 (MQ=255)

                                                                  cGCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGc                       <  1:4426345/93‑1 (MQ=255)

                                                                   gCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCa                      <  2:3558885/93‑1 (MQ=255)

                                                                   gCCCTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCa                      <  1:4946980/93‑1 (MQ=255)

                                                                    cccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAg                     <  2:6976776/93‑1 (MQ=255)

                                                                    cccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAg                     >  2:271532/1‑93 (MQ=255)

                                                                     ccTGCTGGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGc                    <  2:8600208/93‑1 (MQ=255)

                                                                      ctgctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCt                   >  1:4734688/1‑93 (MQ=255)

                                                                      ctgctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGGTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGcg                   >  2:5185667/1‑92 (MQ=255)

                                                                        gctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTgg                 <  2:645119/93‑1 (MQ=255)

                                                                         ctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGt                <  1:1162940/93‑1 (MQ=255)

                                                                         ctgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGt                <  1:5037913/93‑1 (MQ=255)

                                                                          tgGTTTGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTc               >  2:5326925/1‑93 (MQ=255)

                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCt            <  1:6306693/93‑1 (MQ=255)

                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCt            >  1:3127792/1‑93 (MQ=255)

                                                                             tttGCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCt            <  2:1971604/93‑1 (MQ=255)

                                                                                gCCGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGaa         <  1:3249183/93‑1 (MQ=255)

                                                                                 ccGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAg        <  1:3820128/93‑1 (MQ=255)

                                                                                  cGGAATAGCGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAgg       >  1:8401348/1‑93 (MQ=255)

AGCTGTCGCCACGGAAATGATGGGAGATTTCACTTAACCAGAGCATCGCGATACGTTCAGGCTGATCGCCCTGCTGGTTTGCCGGAATAGTGCCCGTCAGCGCATGACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGT  >  NC_000913/3507741‑3507920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`