BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,529,849	T→C	T77A (ACG→GCG)	yhgE ←	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,529,849	0	T	C	99.2%	1505.0 / ‑2.9	472	T77A (ACG→GCG)	yhgE	putative transport
Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (202/266); total (205/267)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 494 total aligned reads displayed.

GATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGG  >  NC_000913/3529759‑3529938

gATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCgg                                                                                         >  2:1915783/1‑93 (MQ=255)

gATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCgg                                                                                         >  1:5123917/1‑93 (MQ=255)

gATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCgg                                                                                         >  1:2094123/1‑93 (MQ=255)

  tAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCa                                                                                       >  1:6323794/1‑93 (MQ=255)

     cAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATa                                                                                    <  1:7764668/93‑1 (MQ=255)

      aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATaa                                                                                   >  1:8883618/1‑93 (MQ=255)

      aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATaa                                                                                   >  2:2991314/1‑93 (MQ=255)

      aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATaa                                                                                   >  1:9429354/1‑93 (MQ=255)

      aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGAGGCAGTaa                                                                                   >  2:6797922/1‑93 (MQ=255)

      aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTACAGCCAGCCGCTCATCCCCAGCGCGGCAATaa                                                                                   <  2:4488802/93‑1 (MQ=255)

       aCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATaaa                                                                                  <  1:8580890/93‑1 (MQ=255)

       aCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATaaa                                                                                  <  2:3882282/93‑1 (MQ=255)

       aCCAAAACCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATaaa                                                                                  <  2:7276430/93‑1 (MQ=255)

        ccAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAac                                                                                 <  2:744679/93‑1 (MQ=255)

        ccAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAac                                                                                 <  2:7513446/93‑1 (MQ=255)

         cAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAaca                                                                                <  1:7668815/93‑1 (MQ=255)

         cAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAaca                                                                                >  1:7409752/1‑93 (MQ=255)

           aaaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACt                                                                              <  1:6958760/93‑1 (MQ=255)

           aaaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACt                                                                              >  1:2610731/1‑93 (MQ=255)

            aaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTa                                                                             <  2:4767915/93‑1 (MQ=255)

            aaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCAGCCCCAGCGCCGCAATACACACTa                                                                             >  2:1734097/1‑93 (MQ=255)

                ccAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAgcgc                                                                         <  2:1604795/93‑1 (MQ=255)

                ccAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAgcgc                                                                         >  1:4780846/1‑93 (MQ=255)

                 cAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCgcc                                                                        <  1:4848110/93‑1 (MQ=255)

                 cAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCgcc                                                                        >  1:7988265/1‑93 (MQ=255)

                     gcgcCTTTGCAGCGCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCgccagcc                                                                    <  1:3992244/93‑1 (MQ=255)

                      cgcCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCgccagcca                                                                   <  2:2587291/93‑1 (MQ=255)

                      cgcCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCgccagcca                                                                   <  1:3608886/93‑1 (MQ=255)

                      cgcCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTCCAGCCAGCCGCTCCTCCCCAGCGCGCCAATAAACACTAGCgccagcca                                                                   <  2:8998720/93‑1 (MQ=255)

                       gcCTTTTCAGCTGTCCAGGGGTGCATGCCAGCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCAt                                                                  <  1:1401877/93‑1 (MQ=255)

                       gcCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCAt                                                                  <  1:8713987/93‑1 (MQ=255)

                        ccTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCTCCAGCGCGGCAATAAACACTAGCGCCAGCCATc                                                                 >  2:5155998/1‑93 (MQ=255)

                        ccTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGACAGCCATc                                                                 >  1:4200085/1‑93 (MQ=255)

                          ttttCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGCCATTAAACACTAGCCCCAGCCAcccc                                                               >  1:8134737/1‑93 (MQ=255)

                           tttCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATcccc                                                              >  1:5760733/1‑93 (MQ=255)

                           tttCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATcccc                                                              <  1:9239986/93‑1 (MQ=255)

                             tCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCaa                                                            >  2:8066555/1‑93 (MQ=255)

                             tCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCaa                                                            <  1:1135956/93‑1 (MQ=255)

                              cAGCTCTCCAGGGGTTCATGCCATCATTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCaaa                                                           <  1:3835674/93‑1 (MQ=255)

                               aGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAg                                                          <  1:8457943/93‑1 (MQ=255)

                                 ctctCCAGGGGTTCATGCCATCAGTTTGCCACTGCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGgc                                                        <  1:9701114/93‑1 (MQ=255)

                                  tctcCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGgcg                                                       >  2:133322/1‑93 (MQ=255)

                                   ctcCAGGGGTTCATGCCATCAGTTTGCCACTTCGGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGgcgc                                                      <  1:6489778/93‑1 (MQ=255)

                                    tcCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCt                                                     >  2:2838503/1‑93 (MQ=255)

                                      cAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTc                                                   >  2:417098/1‑93 (MQ=255)

                                      cAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTc                                                   <  2:1087440/93‑1 (MQ=255)

                                      cAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTc                                                   <  1:5472088/93‑1 (MQ=255)

                                      cAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATACCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTc                                                   >  1:4047865/1‑93 (MQ=255)

                                       aGGCGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCt                                                  <  2:5829762/93‑1 (MQ=255)

                                        ggggTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTg                                                 >  1:4551999/1‑93 (MQ=255)

                                           gTTCATGCCATCCGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGttt                                              >  1:6089324/1‑93 (MQ=255)

                                           gTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGttt                                              >  2:6960339/1‑93 (MQ=255)

                                            ttCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTa                                             >  1:6162946/1‑93 (MQ=255)

                                              cATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTaaa                                           >  1:4988911/1‑93 (MQ=255)

                                                tGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAag                                         <  2:2356616/93‑1 (MQ=255)

                                                 gCCATCAGTTTGCCAATTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGATTCTGTTTAAAaga                                        >  2:673941/1‑93 (MQ=255)

                                                  ccATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAagag                                       >  1:7554481/1‑93 (MQ=255)

                                                  ccATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAagag                                       <  1:4304725/93‑1 (MQ=255)

                                                    aTCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGAt                                     >  2:2733022/1‑93 (MQ=255)

                                                    aTCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAACAGAt                                     <  2:4050172/93‑1 (MQ=255)

                                                      cAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCa                                   <  1:2443998/93‑1 (MQ=255)

                                                       aGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCAc                                  <  2:3585368/93‑1 (MQ=255)

                                                        gTTTGCCACTTCAGCCAGCCGCTCATCCCTAGCGCGGCAATAAACACTAGCGCAAGCCATCCCCAAAGGCGCTACTGTTTAAAAGAGATCACg                                 <  1:9653855/93‑1 (MQ=255)

                                                        gTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACg                                 <  2:9338763/93‑1 (MQ=255)

                                                        gTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACg                                 <  1:6262659/93‑1 (MQ=255)

                                                        gTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACg                                 <  1:3060785/93‑1 (MQ=255)

                                                         tttCCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACgg                                <  2:7847276/93‑1 (MQ=255)

                                                          ttGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGga                               <  2:653141/93‑1 (MQ=255)

                                                          ttGCCACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGga                               <  2:3749768/93‑1 (MQ=255)

                                                             ccACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGagaa                            <  1:9987904/93‑1 (MQ=255)

                                                              cACTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGagaag                           >  2:5411697/1‑93 (MQ=255)

                                                               aCTTCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGagaaga                          >  2:9604524/1‑93 (MQ=255)

                                                                  tCAGCCAGCCGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTa                       <  1:6636082/93‑1 (MQ=255)

                                                                     gccagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTaaaa                    <  1:10007505/93‑1 (MQ=255)

                                                                     gccagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTaaaa                    >  1:5629513/1‑93 (MQ=255)

                                                                     gccagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTaaaa                    >  1:1725309/1‑93 (MQ=255)

                                                                     gccagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTaaaa                    <  1:9064996/93‑1 (MQ=255)

                                                                     gccagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGAaaaa                    >  2:8632028/1‑93 (MQ=255)

                                                                      ccagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAAc                   <  2:6098719/93‑1 (MQ=255)

                                                                       cagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACg                  >  2:8573365/1‑93 (MQ=255)

                                                                       cagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACg                  <  2:2146140/93‑1 (MQ=255)

                                                                       cagccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATACCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGAAAAACg                  >  1:5286307/1‑93 (MQ=255)

                                                                        agccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACgg                 <  2:3241212/93‑1 (MQ=255)

                                                                         gccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGa                <  2:3383067/93‑1 (MQ=255)

                                                                         gccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGa                >  2:9813667/1‑93 (MQ=255)

                                                                         gccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGa                >  2:144168/1‑93 (MQ=255)

                                                                         gccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGa                <  2:4927880/93‑1 (MQ=255)

                                                                         gccGCTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGCAAAACGGa                >  1:1262522/1‑93 (MQ=255)

                                                                         gccGCGCATCCCCAGCGCGGCAATAAACACTAGCAACAGCCATCCCCAAAGGCGCTCCTGTTTAAAAAAGATCACGGAGAAGAGTAAAACGGa                <  1:1600139/93‑1 (MQ=255)

                                                                             cTCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGaa            >  2:8549094/1‑93 (MQ=255)

                                                                              tCATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGaaa           >  2:6325275/1‑93 (MQ=255)

                                                                               cagccccAACGCGTCAATAAACCCTAGCGCCAGCCACCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGaaaa          <  1:2263208/90‑1 (MQ=255)

                                                                               cATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGaaaa          <  1:4680609/93‑1 (MQ=255)

                                                                               cATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGaaaa          <  1:2819081/93‑1 (MQ=255)

                                                                               cATCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGaaaa          <  1:7250427/93‑1 (MQ=255)

                                                                                aTCCCCAGCGCGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAAc         >  1:3470058/1‑93 (MQ=255)

                                                                                   cccAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAAcgcg      >  1:5932977/1‑93 (MQ=255)

                                                                                       gcgcGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACgg  <  2:8219765/93‑1 (MQ=255)

                                                                                       gcgcGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACgg  >  1:288040/1‑93 (MQ=255)

                                                                                       gcgcGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACgg  <  2:9240450/93‑1 (MQ=255)

GATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGG  >  NC_000913/3529759‑3529938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`