BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,533,160	A→G	L116P (CTG→CCG)	envZ ←	protein histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,533,160	0	A	G	100.0%	1049.8 / NA	325	L116P (CTG→CCG)	envZ	protein histidine kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (161/164); total (161/164)

Only 100 of 338 total aligned reads displayed.

CGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGA  >  NC_000913/3533070‑3533248

cgTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGt                                                                                        <  1:2574470/93‑1 (MQ=255)

   tcccAGATATTGGGCGACAGCCAGGTTTTCAGTCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgc                                                                                     <  2:91011/92‑1 (MQ=255)

   aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgc                                                                                     <  1:8391614/93‑1 (MQ=255)

   aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgc                                                                                     <  1:7066611/93‑1 (MQ=255)

   aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgc                                                                                     >  2:6951432/1‑93 (MQ=255)

   aCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgc                                                                                     >  1:2862372/1‑93 (MQ=255)

     ccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCACCGCGCACTTCCGTCGGGCCGCCCGGTtgctg                                                                                   >  2:7611214/1‑93 (MQ=255)

     ccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgctg                                                                                   >  2:2391712/1‑93 (MQ=255)

     ccAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgctg                                                                                   <  2:9828676/93‑1 (MQ=255)

      cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTtgctgc                                                                                  <  1:6629044/93‑1 (MQ=255)

      cAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGAGCACTTCCGTCGGGCCGCCCGGTtgctgc                                                                                  >  2:5198686/1‑93 (MQ=255)

        gATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTgcgc                                                                                <  2:2889267/93‑1 (MQ=255)

        gATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTgcgc                                                                                >  2:124505/1‑93 (MQ=255)

        gATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTgcgc                                                                                >  2:5631753/1‑93 (MQ=255)

        gATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTgcgc                                                                                >  1:2312026/1‑93 (MQ=255)

         atatTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGcc                                                                               <  2:4877625/93‑1 (MQ=255)

         atatTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGcc                                                                               >  2:2633735/1‑93 (MQ=255)

         atatTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGcc                                                                               <  1:6835319/93‑1 (MQ=255)

          tatTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCa                                                                              <  1:500608/93‑1 (MQ=255)

          tatTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCa                                                                              <  1:2020353/93‑1 (MQ=255)

           atTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCAt                                                                             <  2:5905875/93‑1 (MQ=255)

            ttGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATc                                                                            >  2:2844463/1‑93 (MQ=255)

            ttGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATc                                                                            <  2:1216040/93‑1 (MQ=255)

            ttGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATc                                                                            <  2:359122/93‑1 (MQ=255)

             tGGGCGACAGCCAGGTTTACAGCCAGACGACATGCGAGCTTCTGTCGCCCTAAACGCCCACTTCCGTAGGTCCCACCGGTTGCTGCGCAATCt                                                                           <  2:1691652/93‑1 (MQ=255)

              gggCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTg                                                                          <  1:4466679/93‑1 (MQ=255)

               ggCGACAGCCAGGTTTTCAGCCAGATGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGa                                                                         <  2:3886868/93‑1 (MQ=255)

               ggCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGa                                                                         <  2:773505/93‑1 (MQ=255)

                gCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGAt                                                                        >  1:1124531/1‑93 (MQ=255)

                 cGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATg                                                                       >  2:5714160/1‑93 (MQ=255)

                   aCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGc                                                                     <  1:7693473/93‑1 (MQ=255)

                   aCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGc                                                                     <  2:4382586/93‑1 (MQ=255)

                     aGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCtt                                                                   <  1:2655890/93‑1 (MQ=255)

                      gCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTa                                                                  >  1:9902334/1‑93 (MQ=255)

                        cAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAg                                                                >  2:3062211/1‑93 (MQ=255)

                         aGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGa                                                               >  1:859696/1‑93 (MQ=255)

                         aGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGa                                                               >  1:9977642/1‑93 (MQ=255)

                         aGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGa                                                               <  1:357268/93‑1 (MQ=255)

                          ggTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGaa                                                              <  1:8326968/93‑1 (MQ=255)

                           gTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAAt                                                             >  2:9209032/1‑93 (MQ=255)

                              ttCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCa                                                          <  1:4788967/93‑1 (MQ=255)

                               tCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCAt                                                         >  2:7255376/1‑93 (MQ=255)

                               tCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTAAt                                                         >  1:2857730/1‑93 (MQ=255)

                                 aGCCAGACGACAGGCGAACGTGTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAg                                                       <  2:2922036/93‑1 (MQ=255)

                                  gCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgt                                                      >  2:7361689/1‑93 (MQ=255)

                                   ccAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtg                                                     <  2:7892260/93‑1 (MQ=255)

                                    cAGACGACCGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt                                                    <  1:2348671/93‑1 (MQ=255)

                                    cAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt                                                    >  2:9445108/1‑93 (MQ=255)

                                    cAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAgtgt                                                    >  2:8630367/1‑93 (MQ=255)

                                     aGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGtt                                                   <  2:815600/93‑1 (MQ=255)

                                      gacgacAGGCGAACTTTTGTTGTCCTCAACTCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTg                                                  <  1:6760754/93‑1 (MQ=255)

                                      gacgacAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTg                                                  >  1:9109255/1‑93 (MQ=255)

                                      gacgacAGGCGAACTTTTGTTGACCTCAACGCGCACTCCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTg                                                  <  2:8959379/93‑1 (MQ=255)

                                       acgacAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTgc                                                 <  1:60099/93‑1 (MQ=255)

                                       acgacAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTgc                                                 <  2:1997550/93‑1 (MQ=255)

                                            aGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCa                                            >  1:4316130/1‑93 (MQ=255)

                                             ggCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCaa                                           >  2:5377531/1‑93 (MQ=255)

                                               cGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACg                                         <  1:330350/93‑1 (MQ=255)

                                                gAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGc                                        <  1:3998587/93‑1 (MQ=255)

                                                gAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGGTGCGCCATTTCATGGGTTAAGCATTCATAGTGTTGCGCCCCAcca                                        >  1:5927827/1‑91 (MQ=255)

                                                 aaCTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCa                                       >  2:7104118/1‑93 (MQ=255)

                                                   cTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGa                                     >  2:3339478/1‑93 (MQ=255)

                                                    ttttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGAc                                    >  2:6547466/1‑93 (MQ=255)

                                                     tttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGAcc                                   >  2:464074/1‑93 (MQ=255)

                                                     tttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGAcc                                   <  1:1287143/93‑1 (MQ=255)

                                                     tttGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGAcc                                   <  1:8828807/93‑1 (MQ=255)

                                                        gttgACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGc                                <  2:821675/93‑1 (MQ=255)

                                                          tgACCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCct                              >  1:6121791/1‑93 (MQ=255)

                                                            aCCTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGCCCTGCctct                            >  1:1580671/1‑93 (MQ=255)

                                                             ccTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCtt                           >  1:5125908/1‑93 (MQ=255)

                                                             ccTCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCtt                           <  1:8127085/93‑1 (MQ=255)

                                                               tCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCg                         >  2:4514304/1‑93 (MQ=255)

                                                               tCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCg                         >  1:4873585/1‑93 (MQ=255)

                                                               tCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCg                         >  2:6230909/1‑93 (MQ=255)

                                                               tCAACGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCg                         >  2:2139613/1‑93 (MQ=255)

                                                                  aCGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCa                      >  1:1922535/1‑93 (MQ=255)

                                                                  aCGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCa                      >  1:9008049/1‑93 (MQ=255)

                                                                  aCGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCa                      <  2:6712591/93‑1 (MQ=255)

                                                                  aCGCGCACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCa                      >  2:1922215/1‑93 (MQ=255)

                                                                      gcACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCt                  <  1:3896300/93‑1 (MQ=255)

                                                                       cACTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTTGGCAGCCTc                 >  2:8680020/1‑93 (MQ=255)

                                                                        aCTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCg                <  1:5765987/93‑1 (MQ=255)

                                                                        aCTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCg                >  1:5375102/1‑93 (MQ=255)

                                                                         cTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGt               >  2:6067843/1‑93 (MQ=255)

                                                                         cTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGt               >  1:5718631/1‑93 (MQ=255)

                                                                         cTTCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGt               >  1:3128821/1‑93 (MQ=255)

                                                                          ttCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGtt              <  1:3389082/93‑1 (MQ=255)

                                                                           tCCGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTg             <  1:7175814/93‑1 (MQ=255)

                                                                            ccGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTgg            <  1:1414745/93‑1 (MQ=255)

                                                                             cGTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGa           <  1:4646940/93‑1 (MQ=255)

                                                                              gTCGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAg          >  1:1784870/1‑93 (MQ=255)

                                                                                cGGGCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTa        <  2:3472816/93‑1 (MQ=255)

                                                                                 gggCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTag       >  1:7378494/1‑93 (MQ=255)

                                                                                 gggCCGCCCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTag       >  2:4928052/1‑93 (MQ=255)

                                                                                   gccgccCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTagag     >  1:6275685/1‑93 (MQ=255)

                                                                                   gccgccCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTagag     >  2:4172138/1‑93 (MQ=255)

                                                                                      gccCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGgagagaga  >  2:8462520/1‑93 (MQ=255)

                                                                                      gccCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTagagaga  <  1:9522930/93‑1 (MQ=255)

                                                                                      gccCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTagagaga  >  2:105954/1‑93 (MQ=255)

                                                                                      gccCGGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTagagaga  >  2:2286611/1‑93 (MQ=255)

CGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGA  >  NC_000913/3533070‑3533248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`