Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,539,774 G→A A578T (GCC→ACC)  feoB → ferrous iron transport protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,539,7740GA99.5% 1424.8 / NA 401A578T (GCC→ACC) feoBferrous iron transport protein B
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (194/204);  minor base C (0/2);  total (194/207)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 410 total aligned reads displayed.

GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTT  >  NC_000913/3539684‑3539863
                                                                                          |                                                                                         
 cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCa                                                                                        <  1:9520188/93‑1 (MQ=255)
 cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCa                                                                                        <  1:6800992/93‑1 (MQ=255)
    ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATgg                                                                                     >  1:1567010/1‑93 (MQ=255)
    ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATgg                                                                                     <  1:378253/93‑1 (MQ=255)
      gtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCg                                                                                   >  1:8602459/1‑93 (MQ=255)
        tacccGAGTGATCTGCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaa                                                                                 <  2:7389576/90‑1 (MQ=255)
        cAGCCGGGTGGTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaa                                                                                 <  2:4839376/93‑1 (MQ=255)
         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaaa                                                                                <  2:2280057/93‑1 (MQ=255)
         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaaa                                                                                <  2:1693209/93‑1 (MQ=255)
         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGaaa                                                                                <  1:7844018/93‑1 (MQ=255)
          gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaag                                                                               >  1:6244649/1‑93 (MQ=255)
          gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaag                                                                               >  1:4701258/1‑93 (MQ=255)
            cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaagaa                                                                             >  2:9471416/1‑93 (MQ=255)
            cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaagaa                                                                             >  2:8109043/1‑93 (MQ=255)
             gggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAAATGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAaagaag                                                                            <  2:1174452/93‑1 (MQ=255)
              ggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGt                                                                           <  2:4285699/93‑1 (MQ=255)
                tcaTCACCGCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTCCCATGGCGAAAGAAGTgg                                                                         <  1:665269/91‑1 (MQ=255)
                  aTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTa                                                                       <  1:7127365/93‑1 (MQ=255)
                   tcgccccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAg                                                                      <  2:9284976/90‑1 (MQ=255)
                     aCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTg                                                                    >  1:4497796/1‑93 (MQ=255)
                      ccccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTgg                                                                   >  2:6330167/1‑93 (MQ=255)
                       cccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTggg                                                                  >  1:1496431/1‑93 (MQ=255)
                        ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGGAGTGGGt                                                                 >  1:4782911/1‑93 (MQ=255)
                         cGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTa                                                                >  1:5508556/1‑93 (MQ=255)
                            tCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGc                                                             >  1:9098789/1‑93 (MQ=255)
                             cTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACAGTTGGCCTGTTTACAGGTACCCTGGCGAAAGAAGTGGTAGAGGGTACGCt                                                            >  2:7071535/1‑93 (MQ=255)
                              ttCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTc                                                           >  1:563055/1‑93 (MQ=255)
                               tCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                                          >  1:5237959/1‑93 (MQ=255)
                                cAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa                                                         <  1:104410/93‑1 (MQ=255)
                                 aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                                                        <  1:9179802/93‑1 (MQ=255)
                                 aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                                                        >  2:2463333/1‑93 (MQ=255)
                                 aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                                                        <  2:4929013/93‑1 (MQ=255)
                                  aGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAaca                                                       <  1:8726500/93‑1 (MQ=255)
                                   gCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacac                                                      >  2:8860149/1‑93 (MQ=255)
                                   gCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacac                                                      >  1:7432631/1‑93 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                                                    >  1:9922555/1‑93 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                                                    <  1:3635289/93‑1 (MQ=255)
                                     cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTAAacacac                                                    >  2:9015786/1‑91 (MQ=255)
                                      aaTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                                                   >  1:6138785/1‑93 (MQ=255)
                                      aaTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                                                   >  2:1306448/1‑93 (MQ=255)
                                        ttGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct                                                 >  2:9409218/1‑93 (MQ=255)
                                         tGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa                                                <  1:9782026/93‑1 (MQ=255)
                                         tGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa                                                <  2:3274702/93‑1 (MQ=255)
                                           gCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTcca                                              >  1:2106719/1‑93 (MQ=255)
                                           gCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTaca                                              <  2:7729254/93‑1 (MQ=255)
                                            cGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac                                             <  2:975222/93‑1 (MQ=255)
                                            cGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac                                             <  2:5818039/93‑1 (MQ=255)
                                             gTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACAcc                                            >  2:2676430/1‑93 (MQ=255)
                                             gTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACAcc                                            >  1:5028095/1‑93 (MQ=255)
                                              tGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg                                           >  2:6098006/1‑93 (MQ=255)
                                              tGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg                                           >  2:3432689/1‑93 (MQ=255)
                                              tGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg                                           >  1:5999852/1‑93 (MQ=255)
                                                cATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCa                                         <  2:8224205/93‑1 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  2:7763922/1‑93 (MQ=255)
                                                  tGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa                                       >  1:704026/1‑93 (MQ=255)
                                                   gAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGAACGCTCAACACCCTCTACACCGCAGaa                                      >  1:5482225/1‑93 (MQ=255)
                                                      gATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAat                                   <  1:7543065/93‑1 (MQ=255)
                                                       aTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAata                                  <  2:6714641/93‑1 (MQ=255)
                                                       aTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAata                                  <  2:3945353/93‑1 (MQ=255)
                                                       aTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAata                                  <  1:694040/93‑1 (MQ=255)
                                                       aTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAata                                  <  1:6345236/93‑1 (MQ=255)
                                                       aTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAata                                  >  2:283891/1‑93 (MQ=255)
                                                        tAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGACAatat                                 >  1:4002689/1‑93 (MQ=255)
                                                        tAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAatat                                 <  1:4095020/93‑1 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGTCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  2:6597534/93‑1 (MQ=255)
                                                          aCTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTc                               <  2:5607314/93‑1 (MQ=255)
                                                           cTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCa                              <  2:2019318/93‑1 (MQ=255)
                                                           cTGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCa                              >  1:2918752/1‑93 (MQ=255)
                                                            tGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAg                             <  2:861445/93‑1 (MQ=255)
                                                            tGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAg                             >  2:8482238/1‑93 (MQ=255)
                                                            tGGCAGGCAACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAg                             <  1:2366424/93‑1 (MQ=255)
                                                              gcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGa                           <  1:3169483/93‑1 (MQ=255)
                                                              gcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGa                           <  2:3548662/93‑1 (MQ=255)
                                                              gcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGa                           <  1:6365901/93‑1 (MQ=255)
                                                              gcaggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGAAGAAAATATTCAGGa                           >  1:8084257/1‑93 (MQ=255)
                                                               caggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGAc                          <  2:7638645/93‑1 (MQ=255)
                                                                aggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACg                         >  1:7781663/1‑93 (MQ=255)
                                                                 ggcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGa                        <  2:5437239/93‑1 (MQ=255)
                                                                  gcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGGAGTGGGAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGaa                       >  2:5084105/1‑93 (MQ=255)
                                                                  gcaACGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGaa                       >  1:1896380/1‑93 (MQ=255)
                                                                    aaCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAaga                     <  1:6443722/93‑1 (MQ=255)
                                                                    aaCGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAaga                     <  1:10002665/93‑1 (MQ=255)
                                                                      cGGTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGt                   >  1:140319/1‑93 (MQ=255)
                                                                        gTTGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTc                 <  2:3099446/93‑1 (MQ=255)
                                                                         ttGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCa                <  1:2543381/93‑1 (MQ=255)
                                                                         ttGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCa                >  2:4087043/1‑93 (MQ=255)
                                                                         ttGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCa                <  2:8361132/93‑1 (MQ=255)
                                                                          tGGCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCaa               <  1:8340320/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  2:7970105/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  1:9315762/93‑1 (MQ=255)
                                                                            gCCTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATc             <  1:4413142/93‑1 (MQ=255)
                                                                             ccTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATcc            <  1:2603515/93‑1 (MQ=255)
                                                                             ccTGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATcc            <  1:5135958/93‑1 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  1:886917/1‑93 (MQ=255)
                                                                               tGTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCgg          >  2:467170/1‑93 (MQ=255)
                                                                                gTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGc         >  2:10012492/1‑93 (MQ=255)
                                                                                gTTTACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGc         >  2:7123748/1‑93 (MQ=255)
                                                                                 tttACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCa        >  2:9923258/1‑93 (MQ=255)
                                                                                 tttACAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCa        <  1:9623864/93‑1 (MQ=255)
                                                                                     cAGGTACCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAAt    <  2:1455923/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGGACGAAGAGTTCAATCCGGCAGAATTT  >  NC_000913/3539684‑3539863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑