Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,590,502 A→G T189T (ACT→ACC)  b3454 ← ATP‑binding component of leucine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,590,5020AG99.2% 1167.6 / ‑1.9 362T189T (ACT→ACC) b3454ATP‑binding component of leucine transport
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (145/214);  total (147/215)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 372 total aligned reads displayed.

ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591
                                                                                          |                                                                                         
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  2:3070236/93‑1 (MQ=255)
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  1:6389201/93‑1 (MQ=255)
 tCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTc                                                                                        >  1:700928/1‑93 (MQ=255)
  cGGAAGGCACTACAGGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  2:8422287/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:4285124/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:6718267/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:648641/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:3593295/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  2:8081298/93‑1 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGATGAGAAAGATGGTCATc                                                                                     >  1:1835096/1‑93 (MQ=255)
     aaaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcc                                                                                    >  2:3402293/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  >  1:6030525/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  2:248914/93‑1 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  1:1914485/93‑1 (MQ=255)
          aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               <  1:3355300/93‑1 (MQ=255)
            tACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTc                                                                             >  1:2726865/1‑93 (MQ=255)
             aCATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCttg                                                                            >  2:1017613/1‑91 (MQ=255)
             aCATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcg                                                                            <  1:5778213/93‑1 (MQ=255)
               aTGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgcg                                                                          <  1:9922926/93‑1 (MQ=255)
                tGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCgcgc                                                                         >  2:1514514/1‑93 (MQ=255)
                tGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCgcgc                                                                         <  1:852903/93‑1 (MQ=255)
                  gCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAg                                                                       >  1:7851406/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   >  1:7608442/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   >  1:8158026/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   >  1:2209861/1‑93 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  2:9791036/93‑1 (MQ=255)
                          ccAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGa                                                               <  1:94508/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  2:6510795/93‑1 (MQ=255)
                            cgCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             <  2:3511962/92‑1 (MQ=255)
                            aGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             >  2:1379692/1‑93 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  1:538576/93‑1 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCATTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  2:5526040/93‑1 (MQ=255)
                              cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                           <  2:9932858/93‑1 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          >  2:9651702/1‑93 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          <  2:8456385/93‑1 (MQ=255)
                                cGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtgt                                                         <  2:281659/93‑1 (MQ=255)
                                  tAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCg                                                       >  1:2574745/1‑93 (MQ=255)
                                    gCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaa                                                     >  2:608124/1‑93 (MQ=255)
                                    gCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaa                                                     >  1:3152811/1‑93 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGGGTCGAAAAttt                                                >  1:2831102/1‑93 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCACTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAttt                                                >  2:8774659/1‑93 (MQ=255)
                                          aTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                               <  2:1468059/93‑1 (MQ=255)
                                             cGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTg                                            <  1:5000367/93‑1 (MQ=255)
                                             cGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTAATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTg                                            <  2:2926637/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  1:7360110/1‑93 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          <  2:4999149/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  1:10046447/1‑93 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         <  2:5241002/93‑1 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGAAGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         >  2:2709847/1‑93 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        <  1:1209169/93‑1 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        >  1:1650800/1‑93 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        <  1:5698817/93‑1 (MQ=255)
                                                 aGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatg                                        >  1:3309229/1‑93 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       >  1:8193573/1‑93 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       <  2:1621510/93‑1 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     <  1:9218897/93‑1 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     >  2:6828251/1‑93 (MQ=255)
                                                     ttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATaa                                    <  2:7688213/93‑1 (MQ=255)
                                                      tAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAt                                   <  1:2058571/93‑1 (MQ=255)
                                                      tAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAt                                   <  1:834106/93‑1 (MQ=255)
                                                      tAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCACGCGCAGCTGCTCGATGGTGTCGAACATTTGCTGGATGATAAt                                   >  2:4797690/1‑93 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:9110078/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:6805455/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTGCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  2:3839447/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 >  2:6732351/1‑93 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 <  2:2553528/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 <  2:4028135/93‑1 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                >  2:2112792/1‑93 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  2:5091877/93‑1 (MQ=255)
                                                          gcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgc                               <  2:7151484/93‑1 (MQ=255)
                                                           ccTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcg                              <  2:9542028/93‑1 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTTGATGATAATCGgcgc                             >  1:1935497/1‑93 (MQ=255)
                                                             tGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCa                            >  1:4743839/1‑93 (MQ=255)
                                                              ggTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCaa                           <  2:3900168/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          <  2:6434691/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          <  1:5866462/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          >  1:5043696/1‑93 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  1:8119652/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  2:4258409/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAgg                   >  2:1851832/1‑92 (MQ=255)
                                                                      ttCTGCTCGAAGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   >  2:9085683/1‑93 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  2:7434904/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGCGCGAt                >  1:2263955/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  2:4613034/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  1:4392376/93‑1 (MQ=255)
                                                                          gCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATg               >  1:1977588/1‑93 (MQ=255)
                                                                          gCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATg               <  1:3814626/93‑1 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  1:891503/93‑1 (MQ=255)
                                                                              gacgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtc           <  1:6255795/93‑1 (MQ=255)
                                                                              gacgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtc           >  1:5104548/1‑93 (MQ=255)
                                                                               acgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtca          <  1:4110478/93‑1 (MQ=255)
                                                                               acgaGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtca          <  2:8176014/93‑1 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGCGCGAAGGCtcatc        >  2:7957206/1‑93 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatc        <  1:9822360/93‑1 (MQ=255)
                                                                                  agaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatca       <  2:3269092/93‑1 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      <  1:393664/93‑1 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      >  2:5593069/1‑93 (MQ=255)
                                                                                    aaaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAg     <  2:2353697/93‑1 (MQ=255)
                                                                                     aaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc    >  2:734922/1‑93 (MQ=255)
                                                                                      aGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCa   <  1:3014198/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑