BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,596,295	A→G	V332A (GTG→GCG)	b3460 ←	LivJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,596,295	0	A	G	99.8%	1504.8 / ‑5.6	466	V332A (GTG→GCG)	b3460	LivJ
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (200/264); total (200/266)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 489 total aligned reads displayed.

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:8382750/1‑93 (MQ=255)

gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:6023277/1‑93 (MQ=255)

 tgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCgg                                                                                        >  2:1012044/1‑93 (MQ=255)

 tgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACgg                                                                                        <  1:780476/93‑1 (MQ=255)

  gccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGa                                                                                       <  1:2711641/93‑1 (MQ=255)

   ccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAg                                                                                      >  2:384909/1‑93 (MQ=255)

   ccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAg                                                                                      <  2:1538564/93‑1 (MQ=255)

   ccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAg                                                                                      <  1:462668/93‑1 (MQ=255)

     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTAACCgaggagat                                                                                    <  2:8988869/93‑3 (MQ=255)

      ttGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTc                                                                                   <  2:6424433/93‑1 (MQ=255)

      ttGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTc                                                                                   >  1:639671/1‑93 (MQ=255)

      ttGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTc                                                                                   >  1:6344420/1‑93 (MQ=255)

        ggCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGc                                                                                 <  1:115174/93‑1 (MQ=255)

        ggCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGc                                                                                 <  2:6550468/93‑1 (MQ=255)

        gccGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGc                                                                                 <  2:1286152/91‑1 (MQ=255)

         gCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCt                                                                                <  2:1750505/93‑1 (MQ=255)

           gTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCTTTACGGTATCCGCGGAGTTCGCttt                                                                              >  1:7504731/1‑93 (MQ=255)

           gTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              <  2:73113/93‑1 (MQ=255)

            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             >  1:7129249/1‑93 (MQ=255)

             gCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCa                                                                            >  2:2741487/1‑93 (MQ=255)

             gCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCa                                                                            <  2:5337895/93‑1 (MQ=255)

               cAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAgg                                                                          >  1:5044549/1‑93 (MQ=255)

                 gTCAAATACGCCGACCAGAAAACCATTCAGATCGCCTTTCTCATCAAAGCTCAGCGGTCCTAATGCGGTACCCACGCAGTTTGGTTTAAGGTa                                                                        <  2:7495449/93‑1 (MQ=255)

                 gTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTa                                                                        >  2:8545239/1‑93 (MQ=255)

                  tCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAt                                                                       >  2:4777465/1‑93 (MQ=255)

                   cAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAtt                                                                      <  2:6301677/93‑1 (MQ=255)

                     aaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTg                                                                    >  1:5410465/1‑93 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  2:1976866/93‑1 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  1:4779210/93‑1 (MQ=255)

                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  2:475093/93‑1 (MQ=255)

                        aCGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCg                                                                 >  2:7770554/1‑93 (MQ=255)

                         cGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGTGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGa                                                                <  2:9748356/93‑1 (MQ=255)

                           ccGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAtt                                                              <  2:4432000/93‑1 (MQ=255)

                           ccGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAtt                                                              <  2:8754425/93‑1 (MQ=255)

                            cGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                             <  2:6008322/93‑1 (MQ=255)

                            cGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                             >  2:1794816/1‑93 (MQ=255)

                             gAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                                            >  2:322971/1‑93 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCATGTATTTGGCGATTTCa                                                           <  2:6708028/93‑1 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           <  2:9103668/93‑1 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           >  1:8499294/1‑93 (MQ=255)

                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           <  1:23478/93‑1 (MQ=255)

                               aCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAg                                                          >  2:4979304/1‑93 (MQ=255)

                                cTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                         >  1:65487/1‑93 (MQ=255)

                                cTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                         <  1:9736432/93‑1 (MQ=255)

                                 tCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGcc                                                        >  1:2587287/1‑93 (MQ=255)

                                 tCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGcc                                                        <  2:2584272/93‑1 (MQ=255)

                                  cAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       >  1:2519770/1‑93 (MQ=255)

                                  cAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       <  1:10039103/93‑1 (MQ=255)

                                       ccTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCTGGTATTTGGCGATTTCAGCCGGATCg                                                  >  1:8271349/1‑93 (MQ=255)

                                        cTTTCAGATCGCCTTCCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGt                                                 <  2:2457745/93‑1 (MQ=255)

                                          ttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCa                                               <  1:3113280/93‑1 (MQ=255)

                                          ttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCa                                               >  1:5608182/1‑93 (MQ=255)

                                          ttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCa                                               <  2:7104423/93‑1 (MQ=255)

                                           tCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAg                                              <  2:5162440/93‑1 (MQ=255)

                                           tCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAg                                              <  1:9364681/93‑1 (MQ=255)

                                             aGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGAc                                            >  1:3423958/1‑93 (MQ=255)

                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           <  2:3555339/93‑1 (MQ=255)

                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           >  2:5599194/1‑93 (MQ=255)

                                               aTCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTGGCTTTCAGCTATTTGGCGATTTCAGCCGCATCGTCAGACTg                                          >  1:4486002/1‑93 (MQ=255)

                                                tCGCCTTTCTCATCCCAGATCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         <  2:5229647/93‑1 (MQ=255)

                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        <  2:8278233/93‑1 (MQ=255)

                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGGTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        >  1:9201335/1‑93 (MQ=255)

                                                   ccTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                                      >  1:3848304/1‑93 (MQ=255)

                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    <  2:5424260/93‑1 (MQ=255)

                                                        ctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                                 <  2:4581685/93‑1 (MQ=255)

                                                         tcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGccc                                <  2:1393572/93‑1 (MQ=255)

                                                           aTCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGc                              >  2:9363269/1‑93 (MQ=255)

                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             >  2:864817/1‑93 (MQ=255)

                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             <  2:5810230/93‑1 (MQ=255)

                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             <  2:3752559/93‑1 (MQ=255)

                                                             cccAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCt                            <  2:6316590/93‑1 (MQ=255)

                                                             cccAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCt                            <  1:2221135/93‑1 (MQ=255)

                                                               cAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGc                          <  1:8025284/93‑1 (MQ=255)

                                                                aGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCa                         >  1:8651104/1‑93 (MQ=255)

                                                                 ggTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGcca                        >  2:8831913/1‑91 (MQ=255)

                                                                 ggTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        >  1:2095711/1‑93 (MQ=255)

                                                                 ggTCAGCGGTCCCATTACGGAATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGCCTGATTACGGGCCGCCTGCaa                        >  1:5741138/1‑93 (MQ=255)

                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     <  1:6096874/93‑1 (MQ=255)

                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     >  2:3349089/1‑93 (MQ=255)

                                                                     aGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAt                    <  1:3344063/93‑1 (MQ=255)

                                                                      gCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAtt                   <  2:2178478/93‑1 (MQ=255)

                                                                       ctgTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTAAGACTGATTCAGGCCCGCCTGCAAAGATTg                  <  1:7245850/91‑1 (MQ=255)

                                                                       cGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTg                  <  1:1524695/93‑1 (MQ=255)

                                                                        ggTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc                 <  1:4056559/93‑1 (MQ=255)

                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                <  2:6928717/93‑1 (MQ=255)

                                                                            ccATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcg             >  2:9224724/1‑93 (MQ=255)

                                                                            ccATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcg             >  1:6905870/1‑93 (MQ=255)

                                                                              aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAACGATTGCAGctcg           >  2:953270/1‑93 (MQ=255)

                                                                              aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGcgcg           >  1:8698403/1‑93 (MQ=255)

                                                                              aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGcgcg           >  1:4232778/1‑93 (MQ=255)

                                                                               ttACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcgg          >  1:6711795/1‑93 (MQ=255)

                                                                               ttACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcgg          >  1:1825174/1‑93 (MQ=255)

                                                                                 aCGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcggcg        <  2:2915789/93‑1 (MQ=255)

                                                                                   ggTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTa      <  2:8036292/93‑1 (MQ=255)

                                                                                   ggTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTa      <  1:3532512/93‑1 (MQ=255)

                                                                                   ggTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTa      <  2:403039/93‑1 (MQ=255)

                                                                                    gTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAg     <  2:4240036/93‑1 (MQ=255)

                                                                                    gTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGCCCCCCCTGCAAAGATTGCATCGCGGCGTAg     >  1:1702744/1‑93 (MQ=255)

                                                                                     tATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAgg    <  1:7721207/93‑1 (MQ=255)

                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  <  2:9674052/93‑1 (MQ=255)

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`