BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,624,135	A→G	N100N (AAT→AAC)	b3485 ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,624,135	0	A	G	99.8%	1336.7 / ‑5.4	409	N100N (AAT→AAC)	b3485	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (204/204); total (204/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 426 total aligned reads displayed.

AAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATA  >  NC_000913/3624045‑3624224

aaaTGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGtt                                                                                         <  1:3927623/93‑1 (MQ=255)

   tGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTAt                                                                                      >  2:2566596/1‑93 (MQ=255)

   tGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTAt                                                                                      >  2:8795324/1‑93 (MQ=255)

   tGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTAt                                                                                      <  2:2942551/93‑1 (MQ=255)

    gCCTGGCTCATGCGCGTGGCATCGACGTGCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATc                                                                                     <  2:9545933/93‑1 (MQ=255)

      cTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgc                                                                                   <  2:9686500/93‑1 (MQ=255)

      cTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgc                                                                                   <  2:5585015/93‑1 (MQ=255)

      cTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgc                                                                                   <  1:3828214/93‑1 (MQ=255)

       tGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgcg                                                                                  >  1:6662226/1‑93 (MQ=255)

        ggCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGa                                                                                 >  1:8997011/1‑93 (MQ=255)

         gCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGaa                                                                                <  2:599256/93‑1 (MQ=255)

         gCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGAGGAATGTTTATCGCGaa                                                                                <  2:7489193/93‑1 (MQ=255)

            cATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGt                                                                             >  1:5245879/1‑93 (MQ=255)

             aTGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGta                                                                            >  2:130192/1‑93 (MQ=255)

             aTGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGta                                                                            >  1:9619482/1‑93 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           >  2:9925116/1‑93 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           <  2:8047943/93‑1 (MQ=255)

               gcgcGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtata                                                                          <  1:9392822/93‑1 (MQ=255)

                cgcgTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAg                                                                         <  1:4387150/93‑1 (MQ=255)

                  cgTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCg                                                                       >  2:6819747/1‑93 (MQ=255)

                  cgTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCg                                                                       >  2:5654294/1‑93 (MQ=255)

                    tGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTc                                                                     <  2:5028637/93‑1 (MQ=255)

                    tGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTc                                                                     <  1:8358213/93‑1 (MQ=255)

                    tGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTc                                                                     >  1:7986504/1‑93 (MQ=255)

                    tGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTc                                                                     <  2:269455/93‑1 (MQ=255)

                     ggCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTcc                                                                    <  2:1983838/93‑1 (MQ=255)

                     ggCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTcc                                                                    <  2:8562297/93‑1 (MQ=255)

                     ggCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTcc                                                                    <  2:5311083/93‑1 (MQ=255)

                     ggCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTcc                                                                    <  2:8748805/93‑1 (MQ=255)

                      gCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCg                                                                   >  1:480627/1‑93 (MQ=255)

                      gCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCg                                                                   <  2:7370167/93‑1 (MQ=255)

                       cATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCgg                                                                  >  2:5440116/1‑93 (MQ=255)

                        aTCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGc                                                                 <  2:3089956/93‑1 (MQ=255)

                         tCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCg                                                                >  2:9197385/1‑93 (MQ=255)

                         tCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCg                                                                >  1:8091753/1‑93 (MQ=255)

                            aCGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTcc                                                             >  1:4930657/1‑93 (MQ=255)

                            aCGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTcc                                                             <  2:6054407/93‑1 (MQ=255)

                             cGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGATATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCa                                                            >  2:8003063/1‑93 (MQ=255)

                             cGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCa                                                            >  1:2295583/1‑93 (MQ=255)

                             cGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCa                                                            >  1:9089146/1‑93 (MQ=255)

                              gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAg                                                           <  2:4421004/93‑1 (MQ=255)

                              gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAg                                                           >  2:4795257/1‑93 (MQ=255)

                              gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAg                                                           <  1:6407337/93‑1 (MQ=255)

                               ttCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGt                                                          <  2:6713820/93‑1 (MQ=255)

                               ttCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGt                                                          >  2:727328/1‑93 (MQ=255)

                                tCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTc                                                         >  2:1773933/1‑93 (MQ=255)

                                  aCCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCg                                                       <  1:5863525/93‑1 (MQ=255)

                                   ccTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCgg                                                      >  2:1030037/1‑93 (MQ=255)

                                   ccTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCgg                                                      <  1:7020731/93‑1 (MQ=255)

                                      gAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcat                                                   <  2:2325101/93‑1 (MQ=255)

                                      gAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcat                                                   >  1:7495768/1‑93 (MQ=255)

                                       aaTATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatc                                                  <  1:7604771/93‑1 (MQ=255)

                                        atatCCGGCTGGCGTCCGGCGAGGACATCGCGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatcc                                                 <  1:7372256/93‑1 (MQ=255)

                                        atatCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatcc                                                 >  1:3112438/1‑93 (MQ=255)

                                         tatCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatcca                                                >  1:404433/1‑93 (MQ=255)

                                          atCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatccat                                               >  2:9111901/1‑93 (MQ=255)

                                           tCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatccatc                                              <  1:6591704/93‑1 (MQ=255)

                                            ccGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCAtct                                             <  1:780001/93‑1 (MQ=255)

                                              ggCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCa                                           >  2:617547/1‑93 (MQ=255)

                                               gCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCAt                                          <  2:356325/93‑1 (MQ=255)

                                               gCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCAt                                          >  2:3982748/1‑93 (MQ=255)

                                                 tGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcg                                        >  2:8412912/1‑93 (MQ=255)

                                                   gCGTCCGGGGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcggc                                      <  1:7164997/93‑1 (MQ=255)

                                                   gCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcggc                                      <  2:7017253/93‑1 (MQ=255)

                                                     gTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcggcgg                                    >  1:1336843/1‑93 (MQ=255)

                                                        cGGCGAGGCCATCACGCTGAAAATTAGGCGGAATGTTTAGCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGa                                 <  1:3234971/93‑1 (MQ=255)

                                                        cGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGa                                 <  1:2748379/93‑1 (MQ=255)

                                                         ggCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGat                                <  1:5301385/93‑1 (MQ=255)

                                                          gCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatc                               >  1:1945471/1‑93 (MQ=255)

                                                          gCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatc                               >  2:7820492/1‑93 (MQ=255)

                                                          gCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatc                               >  1:3446118/1‑93 (MQ=255)

                                                             aGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatcatc                            <  2:2173038/93‑1 (MQ=255)

                                                             aGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatcatc                            >  1:9834563/1‑93 (MQ=255)

                                                               gACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCAtctc                          >  1:6310736/1‑93 (MQ=255)

                                                               gACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCAtatc                          <  1:6122448/93‑1 (MQ=255)

                                                                aCATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTcc                         >  1:8592037/1‑93 (MQ=255)

                                                                 cATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCg                        >  2:2501517/1‑93 (MQ=255)

                                                                 cATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCg                        <  2:8323310/93‑1 (MQ=255)

                                                                 cATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCg                        >  2:2756300/1‑93 (MQ=255)

                                                                  aTCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCgg                       <  1:2495978/93‑1 (MQ=255)

                                                                   tCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCggg                      >  1:1417177/1‑92 (MQ=255)

                                                                    cACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTg                     >  2:4630789/1‑93 (MQ=255)

                                                                    cACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTg                     <  2:3867435/93‑1 (MQ=255)

                                                                       gCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCa                  >  1:6947741/1‑93 (MQ=255)

                                                                         tGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCaaa                <  2:9031420/93‑1 (MQ=255)

                                                                           aaaaTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCaaaaa              >  2:1488870/1‑93 (MQ=255)

                                                                            aaaTTAGGCGGAATGTTTATCGCGAAGGGATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAAc             >  1:3358895/1‑93 (MQ=255)

                                                                              aTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCa           <  1:6991151/93‑1 (MQ=255)

                                                                              aTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCa           <  2:9365568/93‑1 (MQ=255)

                                                                               ttAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCAc          <  2:3316337/93‑1 (MQ=255)

                                                                                tAGGCGGAATGTCTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGGGATCATCTCCGGCGGCAAAAAACACg         >  2:1215811/1‑93 (MQ=255)

                                                                                  ggCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGa       <  2:1574385/93‑1 (MQ=255)

                                                                                   gCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGAc      <  2:4166388/93‑1 (MQ=255)

                                                                                    cGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACg     >  1:10015582/1‑93 (MQ=255)

                                                                                    cGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACg     >  2:6523661/1‑93 (MQ=255)

                                                                                    cGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCAAGGACg     >  1:4730689/1‑93 (MQ=255)

                                                                                     ggAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGa    <  1:3634537/93‑1 (MQ=255)

                                                                                     ggAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGa    >  1:1257059/1‑93 (MQ=255)

                                                                                      gAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGAt   <  2:3760612/93‑1 (MQ=255)

                                                                                      gAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATACATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGAt   >  2:6352656/1‑93 (MQ=255)

AAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATA  >  NC_000913/3624045‑3624224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`