BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,228	A→G	R271R (CGT→CGC)	yhiN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,228	0	A	G	99.5%	1283.4 / ‑4.9	402	R271R (CGT→CGC)	yhiN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (196/202); minor base T (2/0); total (199/203)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTG  >  NC_000913/3634138‑3634317

gATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCg                                                                                         <  1:3103297/93‑1 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                        >  2:4681523/1‑93 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                        <  2:8556593/93‑1 (MQ=255)

  tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTGGGATGTGCGTTGCgct                                                                                       >  2:5331141/1‑93 (MQ=255)

     gCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctgct                                                                                    >  1:351761/1‑93 (MQ=255)

      cccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTc                                                                                   <  1:1034823/93‑1 (MQ=255)

      cccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTc                                                                                   >  2:5724407/1‑93 (MQ=255)

       ccGAGTTGGTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCa                                                                                  <  2:340989/93‑1 (MQ=255)

       ccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCa                                                                                  <  1:5241778/93‑1 (MQ=255)

        cGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAt                                                                                 <  2:5028429/93‑1 (MQ=255)

          aGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTc                                                                               <  1:9525474/93‑1 (MQ=255)

          aGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTc                                                                               <  1:1281566/93‑1 (MQ=255)

           gTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCa                                                                              <  2:4474059/93‑1 (MQ=255)

           gTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCa                                                                              >  2:1944432/1‑93 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                             >  1:8531868/1‑93 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                             <  1:5495022/93‑1 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGGTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                             >  2:8429949/1‑93 (MQ=255)

              gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                           >  2:7778444/1‑93 (MQ=255)

              gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                           <  1:7480215/93‑1 (MQ=255)

               cTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGaa                                                                          >  1:7730666/1‑93 (MQ=255)

               cTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGaa                                                                          >  2:3834715/1‑93 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                    <  2:5225187/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  1:7541895/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  2:2302662/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  1:8947730/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  >  1:4134807/1‑93 (MQ=255)

                        ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                                 >  1:1401918/1‑93 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                <  1:8723035/93‑1 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                <  1:6688407/93‑1 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                <  2:6220096/93‑1 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  2:4130183/1‑93 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  1:1489053/1‑93 (MQ=255)

                             ccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCca                                                            >  1:5903923/1‑93 (MQ=255)

                             ccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCca                                                            <  1:7895868/93‑1 (MQ=255)

                               aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                          >  1:234554/1‑93 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        <  1:5611281/93‑1 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        <  2:9644003/93‑1 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        <  2:307952/93‑1 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        <  1:8325061/93‑1 (MQ=255)

                                  cGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATcc                                                       <  1:6139275/93‑1 (MQ=255)

                                    cTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCCTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCgg                                                     >  1:1157675/1‑93 (MQ=255)

                                     ttCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    <  2:7700050/93‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   <  2:8670628/93‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   >  2:5550918/1‑93 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   <  2:9768265/93‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   <  1:381122/93‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   <  1:7218558/93‑1 (MQ=255)

                                       cGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAg                                                  <  1:9187855/93‑1 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTTCGCTGCTCATTCAGGAAGGTTTCGCGGTCCACATCCGGTAGCa                                                >  1:4374415/1‑93 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                                >  1:2181474/1‑93 (MQ=255)

                                          tAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAg                                               >  1:4811273/1‑93 (MQ=255)

                                           aGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGa                                              <  1:3940597/93‑1 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          >  2:528151/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          <  1:6287450/93‑1 (MQ=255)

                                                aaCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGAt                                         >  1:4743184/1‑93 (MQ=255)

                                                    gCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTg                                     <  2:3971966/93‑1 (MQ=255)

                                                     ccAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGa                                    >  1:9304072/1‑93 (MQ=255)

                                                     ccAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGa                                    >  1:7002824/1‑93 (MQ=255)

                                                       aGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACa                                  >  2:814553/1‑93 (MQ=255)

                                                        gtgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaa                                 >  2:7887490/1‑93 (MQ=255)

                                                        gtgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaa                                 >  2:1790419/1‑93 (MQ=255)

                                                        gtgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaa                                 <  1:8102772/93‑1 (MQ=255)

                                                         tgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaaa                                >  2:662877/1‑93 (MQ=255)

                                                         tgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaaa                                >  2:7549211/1‑93 (MQ=255)

                                                          gtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAt                               >  1:188100/1‑93 (MQ=255)

                                                          gtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAt                               <  1:4268214/93‑1 (MQ=255)

                                                          gtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAt                               >  2:2600352/1‑93 (MQ=255)

                                                           tgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAtt                              <  2:518872/93‑1 (MQ=255)

                                                            gtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTc                             >  2:6090741/1‑93 (MQ=255)

                                                             tttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcc                            <  1:2911994/93‑1 (MQ=255)

                                                               tttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcccc                          <  2:3694975/93‑1 (MQ=255)

                                                               tttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcccc                          >  1:9410982/1‑93 (MQ=255)

                                                                ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                         <  2:2082566/93‑1 (MQ=255)

                                                                 tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                        >  2:4047390/1‑93 (MQ=255)

                                                                 tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                        <  2:1522461/93‑1 (MQ=255)

                                                                 tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                        >  1:2836904/1‑93 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      >  1:6507526/1‑93 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      >  1:9957944/1‑93 (MQ=255)

                                                                    ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                     >  2:5126363/1‑93 (MQ=255)

                                                                    ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                     >  2:7284204/1‑93 (MQ=255)

                                                                      cTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGc                   >  2:2839341/1‑93 (MQ=255)

                                                                        ttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCa                 <  1:1679925/93‑1 (MQ=255)

                                                                        ttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCa                 <  1:8912480/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  2:9342292/93‑1 (MQ=255)

                                                                            ttCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAg             >  2:6777290/1‑93 (MQ=255)

                                                                             tCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGc            <  2:6484938/93‑1 (MQ=255)

                                                                              cGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCt           <  2:3177175/93‑1 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          <  1:9713438/93‑1 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          >  1:399109/1‑93 (MQ=255)

                                                                               ggATGTGCGTTGAGCTGCTCATTCTGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGTTGACAAATTCGCCCGGCTGCCAGTAGCtt          >  2:2966125/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGTCAGTAGCTTg         >  2:4802825/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg         >  1:7686750/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg         <  2:5977998/93‑1 (MQ=255)

                                                                                  tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa       >  1:3379319/1‑93 (MQ=255)

                                                                                   gtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaaa      <  2:2682121/93‑1 (MQ=255)

                                                                                    tgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAAt     <  1:7399106/93‑1 (MQ=255)

                                                                                    tgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAAt     <  2:9017090/93‑1 (MQ=255)

                                                                                     gCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATc    <  2:1368353/93‑1 (MQ=255)

                                                                                      cGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCt   <  2:7086062/93‑1 (MQ=255)

                                                                                       gTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTg  <  1:2620425/93‑1 (MQ=255)

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTG  >  NC_000913/3634138‑3634317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`