BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,679,306	T→C	S89G (AGC→GGC)	yhjJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,679,306	0	T	C	99.8%	1378.0 / NA	433	S89G (AGC→GGC)	yhjJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (207/225); total (207/226)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 449 total aligned reads displayed.

CATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCTTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAA  >  NC_000913/3679216‑3679395

cATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCctt                                                                                         <  1:7186257/93‑1 (MQ=255)

  tgagagACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGc                                                                                       >  2:1654619/3‑93 (MQ=255)

  tAAGAGACAATTACCGGCGGCATCGGGCGGTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGc                                                                                       >  1:9885415/1‑93 (MQ=255)

   aaGAGACAATTCCCGGCGGCATCGGGCTTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCg                                                                                      <  2:8534062/93‑1 (MQ=255)

   aaGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCg                                                                                      <  2:589850/93‑1 (MQ=255)

   aaGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCg                                                                                      >  2:3701240/1‑93 (MQ=255)

     gagaCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGtt                                                                                    >  2:9789850/1‑93 (MQ=255)

       gaCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAg                                                                                  >  1:9085329/1‑93 (MQ=255)

        aCAATTGCCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAgc                                                                                 <  1:1604253/93‑1 (MQ=255)

        aCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAgc                                                                                 >  1:1964610/1‑93 (MQ=255)

           aTTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCa                                                                              >  1:6782498/1‑93 (MQ=255)

            ttcccGGCGGCATCGGGCGTTTAGGGGCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCaa                                                                             <  2:9697141/90‑1 (MQ=255)

             tACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAAt                                                                            <  1:9287275/93‑1 (MQ=255)

              aCCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATa                                                                           <  2:8399886/93‑1 (MQ=255)

              aCCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATa                                                                           <  2:7609475/93‑1 (MQ=255)

               cgggcggcACCGGGCGTGTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATAc                                                                          <  2:7313650/91‑1 (MQ=255)

               ccGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATAc                                                                          <  1:3325677/93‑1 (MQ=255)

                cggcggCATCTGGCGTGTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACg                                                                         <  2:1408859/93‑1 (MQ=255)

                cggcggCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCGTGCGTTAGCGCAATACg                                                                         >  1:8965420/1‑93 (MQ=255)

                 ggcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGa                                                                        <  2:5666874/93‑1 (MQ=255)

                 ggcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGa                                                                        <  2:2548876/93‑1 (MQ=255)

                 ggcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATAAGa                                                                        <  2:2846146/93‑1 (MQ=255)

                  gcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCCAGGCCACCGCCTTGCGTTAGCGCAATACGAg                                                                       >  1:3694435/1‑93 (MQ=255)

                  gcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAg                                                                       >  1:5893432/1‑93 (MQ=255)

                  gcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAg                                                                       >  2:8655901/1‑93 (MQ=255)

                   cggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAgg                                                                      <  1:239343/93‑1 (MQ=255)

                   cggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAgg                                                                      <  1:2960376/93‑1 (MQ=255)

                   cggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAgg                                                                      <  1:274391/93‑1 (MQ=255)

                   cggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAgg                                                                      <  1:7272428/93‑1 (MQ=255)

                   cggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAgg                                                                      <  1:4153362/93‑1 (MQ=255)

                     gcATCGGGCGTTTAGGGTCGATCGCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  2:5503326/93‑1 (MQ=255)

                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  1:397225/93‑1 (MQ=255)

                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    >  1:3072706/1‑93 (MQ=255)

                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  2:8202110/93‑1 (MQ=255)

                      cATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGAt                                                                   <  1:5812246/93‑1 (MQ=255)

                       aTCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATg                                                                  <  1:8077218/93‑1 (MQ=255)

                       aTCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATg                                                                  >  2:5006384/1‑93 (MQ=255)

                        tCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATgg                                                                 >  2:3137781/1‑93 (MQ=255)

                          gggCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCg                                                               >  1:6131066/1‑93 (MQ=255)

                          gggCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCg                                                               >  1:4461813/1‑93 (MQ=255)

                             cGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGa                                                            >  1:4113176/1‑93 (MQ=255)

                              gTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGAc                                                           <  1:7514323/93‑1 (MQ=255)

                              gTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGCGAc                                                           >  2:3237156/1‑93 (MQ=255)

                               tttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACt                                                          >  1:4947735/1‑93 (MQ=255)

                                ttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTg                                                         >  2:5390482/1‑93 (MQ=255)

                                 tAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGt                                                        >  1:4348643/1‑93 (MQ=255)

                                  aGGTTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTa                                                       <  1:9501986/93‑1 (MQ=255)

                                   gggTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCACTACGAGGGATGGCGTGACTGTaa                                                      >  1:4720113/1‑93 (MQ=255)

                                     gTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAcc                                                    >  1:6879113/1‑93 (MQ=255)

                                     gTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAcc                                                    >  2:6306419/1‑93 (MQ=255)

                                     gTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAcc                                                    >  2:679529/1‑93 (MQ=255)

                                       gtgtCCCCTGCTGCCACAAAGAACGCGCCTGTGCTGGGTCAAGGCCACCGCCTTGCGTTAGCGCAATAGGAGGGATGGCGTGACTGTAACCGc                                                  <  2:4585585/90‑1 (MQ=255)

                                        gATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGct                                                 >  2:229595/1‑93 (MQ=255)

                                         aTCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGctc                                                >  2:5302388/1‑93 (MQ=255)

                                          tCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGctct                                               >  1:5522512/1‑93 (MQ=255)

                                             ccTGCTGCCACAATGAACGCGCCTGTGCTGCGCCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGAGGGCGTGACTGTAACCCCTCtgtg                                            >  1:6245870/1‑92 (MQ=255)

                                              ctgctgCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCtgttg                                           <  2:10019815/93‑1 (MQ=255)

                                              cggctgcCACAATGAACGCGCCTGTGCTGCCTCAAGGCCGCCGCCTTGCGTTAGTGCAATACGAGGGATGGCGTGACTGTAACCGCTCtgttg                                           <  2:1502323/91‑1 (MQ=255)

                                                gctgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTtgtg                                         >  1:2183694/1‑93 (MQ=255)

                                                  tgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTa                                       <  1:4279212/93‑1 (MQ=255)

                                                  tgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTa                                       >  2:891258/1‑93 (MQ=255)

                                                  tgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTa                                       <  1:1739279/93‑1 (MQ=255)

                                                  tgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTa                                       <  1:7812432/93‑1 (MQ=255)

                                                   gcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTAc                                      <  2:9661368/93‑1 (MQ=255)

                                                    ccACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACt                                     <  2:9439858/93‑1 (MQ=255)

                                                    ccACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACt                                     <  1:8512863/93‑1 (MQ=255)

                                                    ccACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACt                                     <  1:3489720/93‑1 (MQ=255)

                                                     cacaATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACtt                                    >  1:8336114/1‑93 (MQ=255)

                                                      acaATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACttt                                   <  1:3147334/93‑1 (MQ=255)

                                                       caATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTc                                  <  2:1153270/93‑1 (MQ=255)

                                                       caATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTc                                  <  1:7881577/93‑1 (MQ=255)

                                                          tGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGgc                               >  2:1806648/1‑93 (MQ=255)

                                                            aaCGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGgcga                             >  2:7075493/1‑93 (MQ=255)

                                                                 gcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGaa                        <  2:582041/93‑1 (MQ=255)

                                                                 gcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGaa                        <  1:6319664/93‑1 (MQ=255)

                                                                 gcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGaa                        <  2:6741752/93‑1 (MQ=255)

                                                                   cTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAAcc                      >  1:6488671/1‑93 (MQ=255)

                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     >  2:5196828/1‑93 (MQ=255)

                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     >  1:7356433/1‑93 (MQ=255)

                                                                     gtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCgg                    >  2:8605154/1‑93 (MQ=255)

                                                                     gtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCgg                    <  1:1186335/93‑1 (MQ=255)

                                                                     gtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCgg                    >  2:5989313/1‑93 (MQ=255)

                                                                     gtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCgg                    >  1:8637514/1‑93 (MQ=255)

                                                                       gctgcGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTa                  <  2:6128549/93‑1 (MQ=255)

                                                                           cGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTaa              <  1:7055592/93‑1 (MQ=255)

                                                                            gTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAc             >  2:7495256/1‑93 (MQ=255)

                                                                             tCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAcc            <  2:2113617/93‑1 (MQ=255)

                                                                             tCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAcc            <  2:2942091/93‑1 (MQ=255)

                                                                             tCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAcc            <  2:7947148/93‑1 (MQ=255)

                                                                              cAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACca           >  1:8404986/1‑93 (MQ=255)

                                                                                 ggCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACcagca        >  2:4882500/1‑93 (MQ=255)

                                                                                 ggCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACcagca        >  1:1017198/1‑93 (MQ=255)

                                                                                    cACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGc     >  2:4413464/1‑93 (MQ=255)

                                                                                    cACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGc     >  2:4198608/1‑93 (MQ=255)

                                                                                    cACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGc     >  2:6003988/1‑93 (MQ=255)

                                                                                    cACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGc     >  2:6439435/1‑93 (MQ=255)

                                                                                     cccGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCg    <  1:5394230/92‑1 (MQ=255)

                                                                                     aCCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCg    >  1:130296/1‑93 (MQ=255)

                                                                                      ccGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGa   <  2:9172101/93‑1 (MQ=255)

                                                                                       cGCCTTGCGTTAGCGGAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGaa  <  2:2698060/93‑1 (MQ=255)

CATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCTTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAA  >  NC_000913/3679216‑3679395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`