BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,683,091	A→G	V42A (GTC→GCC)	yhjK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,091	0	A	G	99.8%	1338.3 / NA	412	V42A (GTC→GCC)	yhjK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (217/194); minor base T (0/1); total (217/195)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

CTGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGACAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAA  >  NC_000913/3683001‑3683180

cTGACCCCGCCTGCCGATTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCa                                                                                         <  2:7260146/93‑1 (MQ=255)

cTGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCa                                                                                         <  2:5517016/93‑1 (MQ=255)

 tGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCag                                                                                        <  1:10006969/93‑1 (MQ=255)

 tGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCag                                                                                        >  1:420997/1‑93 (MQ=255)

  gACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       <  1:9941404/93‑1 (MQ=255)

  gACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       >  1:3013998/1‑93 (MQ=255)

   aCCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCagag                                                                                      <  2:6117119/93‑1 (MQ=255)

   aCCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCagag                                                                                      <  1:1474861/93‑1 (MQ=255)

     cACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcg                                                                                    >  2:6980687/1‑93 (MQ=255)

      aCGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcga                                                                                   >  2:351420/1‑93 (MQ=255)

        gCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcgagc                                                                                 <  2:2590465/93‑1 (MQ=255)

           tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              >  1:9322449/1‑93 (MQ=255)

           tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              >  1:6341361/1‑93 (MQ=255)

           tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              <  2:301157/93‑1 (MQ=255)

           tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              >  2:1339916/1‑93 (MQ=255)

             ccGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGc                                                                            <  2:2046714/93‑1 (MQ=255)

               ggTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCtt                                                                          >  1:863420/1‑93 (MQ=255)

                gTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCttt                                                                         >  2:41363/1‑93 (MQ=255)

                 tttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTc                                                                        <  1:2894000/93‑1 (MQ=255)

                  ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTcc                                                                       >  1:9192008/1‑93 (MQ=255)

                  ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTcc                                                                       >  2:9463604/1‑93 (MQ=255)

                  ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTGTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTcc                                                                       >  1:5430073/1‑93 (MQ=255)

                   tAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCa                                                                      >  2:9799396/1‑93 (MQ=255)

                    aaTGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAg                                                                     >  2:3260795/1‑93 (MQ=255)

                     aTGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGt                                                                    >  2:1622213/1‑93 (MQ=255)

                      tGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGtt                                                                   >  1:837304/1‑93 (MQ=255)

                        cTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGc                                                                 >  2:4021342/1‑93 (MQ=255)

                          ggCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGt                                                               <  2:8848447/93‑1 (MQ=255)

                          ggCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGt                                                               >  2:1817769/1‑93 (MQ=255)

                           gCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTa                                                              <  1:6466628/93‑1 (MQ=255)

                           gCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTa                                                              >  2:9569403/1‑93 (MQ=255)

                            cAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAg                                                             <  1:3207835/93‑1 (MQ=255)

                            cAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAg                                                             >  1:3901614/1‑93 (MQ=255)

                             aaGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGc                                                            <  2:1012435/93‑1 (MQ=255)

                              aGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAGTGCTTTCCAGTTGCGTAGcc                                                           >  2:5843429/1‑93 (MQ=255)

                              aGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGcc                                                           >  2:7116215/1‑93 (MQ=255)

                               gAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCg                                                          >  1:4453581/1‑93 (MQ=255)

                               gAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCg                                                          <  2:5120991/93‑1 (MQ=255)

                               gAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCg                                                          >  1:7031493/1‑93 (MQ=255)

                               gAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCg                                                          >  2:8389249/1‑93 (MQ=255)

                                 aTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgtg                                                        >  1:8478604/1‑93 (MQ=255)

                                  tAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgtgt                                                       >  2:2465939/1‑93 (MQ=255)

                                    gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     <  1:1814910/93‑1 (MQ=255)

                                    gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     >  2:6381294/1‑93 (MQ=255)

                                      ttCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGTGTAGCCGTGTtata                                                   <  1:7464557/93‑1 (MQ=255)

                                       tCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAg                                                  <  2:7773058/93‑1 (MQ=255)

                                       tCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAg                                                  >  2:2796860/1‑93 (MQ=255)

                                        ccGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGc                                                 <  2:5375178/93‑1 (MQ=255)

                                          gCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGa                                               >  1:7895320/1‑93 (MQ=255)

                                           cTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGAt                                              <  1:1958118/93‑1 (MQ=255)

                                             tCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTc                                            <  2:3875663/93‑1 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         >  2:8048166/1‑93 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         >  1:5909046/1‑93 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         >  1:6265221/1‑93 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         >  2:3621558/1‑93 (MQ=255)

                                                 gAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgct                                        >  2:6572604/1‑93 (MQ=255)

                                                 gAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgct                                        <  1:3489179/93‑1 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     >  1:4017627/1‑93 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     >  2:2325726/1‑93 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTACCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     >  1:1233156/1‑93 (MQ=255)

                                                     atCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAc                                    <  2:4265582/93‑1 (MQ=255)

                                                      tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   >  2:5292485/1‑93 (MQ=255)

                                                      gcGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   <  1:3236467/92‑1 (MQ=255)

                                                       cGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCa                                  <  2:4495317/93‑1 (MQ=255)

                                                       cGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCa                                  >  1:8121173/1‑93 (MQ=255)

                                                        gCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAg                                 >  1:9510165/1‑93 (MQ=255)

                                                        gCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAg                                 >  1:2383731/1‑93 (MQ=255)

                                                         ccTTTCAAAATAGCTGAAGATAAGGGGTCGCGGGCAGAGCGAGCAATGCTTTACAGGCGAGTAGCCGGGTTATAGCGATTCTGCTGGAGCAgg                                >  2:89289/1‑92 (MQ=255)

                                                          cTTTCAAAATAGTTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATGGCGATGCTGCTGGACCAgct                               >  2:1212118/1‑91 (MQ=255)

                                                          cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               <  2:2287142/93‑1 (MQ=255)

                                                          cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               >  2:4761121/1‑93 (MQ=255)

                                                           tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              >  2:9237719/1‑93 (MQ=255)

                                                           tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              <  1:5571205/93‑1 (MQ=255)

                                                           tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              >  1:6682216/1‑93 (MQ=255)

                                                           tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGCTATAGCGATTCTGCTGGACCAGATg                              >  1:8932156/1‑93 (MQ=255)

                                                             tCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGa                            >  1:9603771/1‑93 (MQ=255)

                                                                aaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACa                         <  2:6751526/93‑1 (MQ=255)

                                                                aaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACa                         <  2:681554/93‑1 (MQ=255)

                                                                 aaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAg                        >  2:9075478/1‑93 (MQ=255)

                                                                 aaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAg                        <  1:6890712/93‑1 (MQ=255)

                                                                 aaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAg                        >  2:9634166/1‑93 (MQ=255)

                                                                  aTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGc                       >  1:9842679/1‑93 (MQ=255)

                                                                    aGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaa                     <  1:7589588/93‑1 (MQ=255)

                                                                      cTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaaaa                   <  1:2700689/93‑1 (MQ=255)

                                                                      cTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaaaa                   >  2:7852183/1‑93 (MQ=255)

                                                                       tGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAAc                  >  1:2158558/1‑93 (MQ=255)

                                                                       tGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAAc                  <  1:4931934/93‑1 (MQ=255)

                                                                        gAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACg                 >  1:3746881/1‑93 (MQ=255)

                                                                        gAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACg                 <  1:4688595/93‑1 (MQ=255)

                                                                        gAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCCTTCCAGTTGCGGAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACg                 <  1:7217226/93‑1 (MQ=255)

                                                                           gATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTg              >  2:3168145/1‑93 (MQ=255)

                                                                            aTAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGc             <  2:7589978/93‑1 (MQ=255)

                                                                            aTAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAACCGTGTTATAGCGATTCTGCTGGAGCAGATGGAACAGCAAAACGGTGc             >  1:9063745/1‑93 (MQ=255)

                                                                             tAAGGGTTCGCGGGCAGAGCGAGCAATTCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGGGCa            >  1:276966/1‑93 (MQ=255)

                                                                              aaGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCaa           <  1:886376/93‑1 (MQ=255)

                                                                                 ggTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCaaaaa        >  2:4872348/1‑93 (MQ=255)

                                                                                   ttCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCaaaaaca      >  1:3337583/1‑91 (MQ=255)

                                                                                     cgcgGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATaa    >  1:4648133/1‑93 (MQ=255)

                                                                                      gcgGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATaaa   <  1:6057320/93‑1 (MQ=255)

                                                                                      gcgGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATaaa   <  2:10038550/93‑1 (MQ=255)

CTGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGACAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAA  >  NC_000913/3683001‑3683180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`