BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,739,740	A→G	M141T (ATG→ACG)	yiaJ ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,739,740	0	A	G	99.8%	1403.2 / ‑6.0	437	M141T (ATG→ACG)	yiaJ	putative regulator
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (224/212); total (224/213)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 459 total aligned reads displayed.

TAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTC  >  NC_000913/3739650‑3739829

tAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTc                                                                                         <  1:3657168/93‑1 (MQ=255)

 aGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTcc                                                                                        <  1:417324/93‑1 (MQ=255)

  gTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTccc                                                                                       <  2:1115611/93‑1 (MQ=255)

   tCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCg                                                                                      >  1:6345087/1‑93 (MQ=255)

     cGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGt                                                                                    <  2:6607804/93‑1 (MQ=255)

      gggTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGtt                                                                                   <  2:5821103/93‑1 (MQ=255)

      gggTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGtt                                                                                   <  2:8641690/93‑1 (MQ=255)

       ggTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtg                                                                                  >  2:5291398/1‑93 (MQ=255)

        gTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgt                                                                                 <  1:3929143/93‑1 (MQ=255)

         tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                <  2:8327257/93‑1 (MQ=255)

          gACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTgg                                                                               >  1:554535/1‑93 (MQ=255)

          gACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTgg                                                                               <  1:81627/93‑1 (MQ=255)

           aCCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTggg                                                                              >  1:6798489/1‑93 (MQ=255)

            ccAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGt                                                                             <  2:5230863/93‑1 (MQ=255)

              aaaCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTc                                                                           >  2:4914503/1‑93 (MQ=255)

               aaCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTcc                                                                          >  1:2161017/1‑93 (MQ=255)

                aCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCa                                                                         <  2:9080751/93‑1 (MQ=255)

                aCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCa                                                                         <  1:1301698/93‑1 (MQ=255)

                aCGCCATGTAGAGCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCa                                                                         <  2:5957794/93‑1 (MQ=255)

                 cGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAg                                                                        <  1:2699636/93‑1 (MQ=255)

                 cGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAg                                                                        >  2:10008189/1‑93 (MQ=255)

                  gCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGc                                                                       >  2:6458107/1‑93 (MQ=255)

                  gCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGc                                                                       >  2:8766358/1‑93 (MQ=255)

                  gCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGc                                                                       >  2:4645111/1‑93 (MQ=255)

                   ccATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGCTCCAGCt                                                                      >  2:5516188/1‑93 (MQ=255)

                     aTGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTta                                                                    >  1:247634/1‑93 (MQ=255)

                      tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTGCCGGTTGTGGGATCCAGCTtat                                                                   >  2:7019459/1‑93 (MQ=255)

                      tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  1:9139000/1‑93 (MQ=255)

                      tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  1:5134375/1‑93 (MQ=255)

                      tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  1:4370952/1‑93 (MQ=255)

                      tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  2:3579445/1‑93 (MQ=255)

                      tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  1:800867/1‑93 (MQ=255)

                       gTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtata                                                                  >  1:6174884/1‑93 (MQ=255)

                        tAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGGTCGCAGCGTCCCGGTTGTGGGGTCCAGCTTCTaa                                                                 >  1:7564923/1‑93 (MQ=255)

                         aGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATaaa                                                                >  1:693669/1‑93 (MQ=255)

                           agcgtGCCCATTGTGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATc                                                              <  2:8086626/89‑1 (MQ=255)

                           aTCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATc                                                              >  2:2765944/1‑93 (MQ=255)

                           aTCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATc                                                              <  2:5599539/93‑1 (MQ=255)

                              ttGCCGATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCaaa                                                           <  2:2926584/93‑1 (MQ=255)

                              ttGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCaaa                                                           >  1:3313432/1‑93 (MQ=255)

                                gCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAAt                                                         <  2:3053669/93‑1 (MQ=255)

                                gCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAAt                                                         <  1:9003575/93‑1 (MQ=255)

                                gCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAAt                                                         <  1:422295/93‑1 (MQ=255)

                                 cccATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATa                                                        >  1:9458250/1‑93 (MQ=255)

                                 cccATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATa                                                        <  1:34110/93‑1 (MQ=255)

                                  ccATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAg                                                       <  2:3316803/93‑1 (MQ=255)

                                   cATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGc                                                      <  2:6899722/93‑1 (MQ=255)

                                     ttGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGt                                                    <  1:1224260/93‑1 (MQ=255)

                                     ttGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGt                                                    >  2:201340/1‑93 (MQ=255)

                                     ttGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGt                                                    >  1:654868/1‑93 (MQ=255)

                                     ttGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGt                                                    >  1:6684490/1‑93 (MQ=255)

                                      tGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTg                                                   >  2:6045530/1‑93 (MQ=255)

                                        cGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGAt                                                 <  1:1665497/93‑1 (MQ=255)

                                          gAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCg                                               >  2:7427583/1‑93 (MQ=255)

                                           aaCAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGt                                              >  2:9620337/1‑93 (MQ=255)

                                            aCAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTc                                             >  2:2700487/1‑93 (MQ=255)

                                               gTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTc                                          >  1:4791883/1‑93 (MQ=255)

                                                 agagCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTcgc                                        <  2:2484627/93‑1 (MQ=255)

                                                    gCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGc                                     >  2:3880751/1‑93 (MQ=255)

                                                      ggCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTg                                   <  1:2813131/93‑1 (MQ=255)

                                                      ggCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTg                                   >  2:1945574/1‑93 (MQ=255)

                                                      ggCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTg                                   <  2:4566296/93‑1 (MQ=255)

                                                       gCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTgg                                  <  2:7825324/93‑1 (MQ=255)

                                                        cATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGga                                 <  2:1846162/93‑1 (MQ=255)

                                                        cATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTACCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGga                                 <  2:7940821/93‑1 (MQ=255)

                                                         atatGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTgggg                                >  1:1404122/1‑91 (MQ=255)

                                                          tatGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGgcga                               >  1:6903188/1‑93 (MQ=255)

                                                          tatGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGgaga                               >  2:3729341/1‑93 (MQ=255)

                                                            tGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAg                             <  1:8856937/93‑1 (MQ=255)

                                                              cTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGtt                           >  2:6217766/1‑93 (MQ=255)

                                                              cTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGtt                           <  1:9358192/93‑1 (MQ=255)

                                                               tGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTa                          <  2:2858831/93‑1 (MQ=255)

                                                               tGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTa                          <  2:1617889/93‑1 (MQ=255)

                                                                ggCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCGAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTaa                         <  1:1052493/93‑1 (MQ=255)

                                                                ggCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTaa                         <  1:1589986/93‑1 (MQ=255)

                                                                 gCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAAt                        >  1:2997849/1‑93 (MQ=255)

                                                                  ccAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGCAGTTAATg                       >  1:4504126/1‑93 (MQ=255)

                                                                  ccAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATg                       <  2:9423689/93‑1 (MQ=255)

                                                                   cAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATgg                      >  2:2258999/1‑93 (MQ=255)

                                                                    aaTATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGt                     >  2:2343990/1‑93 (MQ=255)

                                                                      tataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGttt                   >  2:6725029/1‑93 (MQ=255)

                                                                       ataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGCAGTTAATGGTTTc                  >  2:1271406/1‑93 (MQ=255)

                                                                       ataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTc                  <  2:4239013/93‑1 (MQ=255)

                                                                       ataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGGTTc                  >  2:7239550/1‑93 (MQ=255)

                                                                        tgggCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCa                 <  1:869141/91‑1 (MQ=255)

                                                                        taGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCa                 <  2:9837929/93‑1 (MQ=255)

                                                                        taGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCa                 <  2:6344029/93‑1 (MQ=255)

                                                                         aGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCAc                <  1:5993451/93‑1 (MQ=255)

                                                                          ggCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCAcc               <  1:7865039/93‑1 (MQ=255)

                                                                          ggCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCAcc               <  2:5676520/93‑1 (MQ=255)

                                                                            cACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAg             <  2:3161209/93‑1 (MQ=255)

                                                                             aCGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGt            <  2:4007973/93‑1 (MQ=255)

                                                                              cGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTg           >  1:5262195/1‑93 (MQ=255)

                                                                              cGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTg           <  1:718314/93‑1 (MQ=255)

                                                                              cGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTg           >  1:3526443/1‑93 (MQ=255)

                                                                               gggTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTgg          <  1:7450020/93‑1 (MQ=255)

                                                                               gggTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTgg          <  2:4845297/93‑1 (MQ=255)

                                                                               gggTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTAATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTgg          <  1:9696502/93‑1 (MQ=255)

                                                                                  gtCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGa       <  2:8531691/92‑1 (MQ=255)

                                                                                     gcagcgTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGt    <  1:1950225/93‑1 (MQ=255)

TAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTC  >  NC_000913/3739650‑3739829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`