BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,744,796	G→A	G28D (GGC→GAC)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,744,796	0	G	A	99.2%	1228.2 / ‑4.0	358	G28D (GGC→GAC)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (165/189); total (169/189)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 398 total aligned reads displayed.

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA  >  NC_000913/3744706‑3744888

aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACg                                                                                            >  2:1710373/1‑93 (MQ=255)

 aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGt                                                                                           <  1:4891374/93‑1 (MQ=255)

  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTg                                                                                          >  2:9758462/1‑93 (MQ=255)

  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTg                                                                                          >  2:6102623/1‑93 (MQ=255)

    aaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCa                                                                                        >  2:5423872/1‑93 (MQ=255)

     aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAg                                                                                       <  2:8281296/93‑1 (MQ=255)

      ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgc                                                                                      >  1:5318203/1‑93 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   <  1:1018823/93‑1 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   >  2:4758860/1‑93 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   >  2:9405323/1‑93 (MQ=255)

           aCGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTg                                                                                 <  1:3470838/93‑1 (MQ=255)

             gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGcc                                                                               <  2:8581696/93‑1 (MQ=255)

             gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGcc                                                                               >  1:5145900/1‑93 (MQ=255)

               aaTTCTGGCTGGGGTTAGATTGTGGCGTTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCGGGGAGGCAGACGTGCAGCTCCTGCCGc                                                                             <  2:6026562/93‑1 (MQ=255)

               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             <  1:8502760/93‑1 (MQ=255)

               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             >  1:3635575/1‑93 (MQ=255)

               aaTACTGGATGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             <  2:891982/93‑1 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                           <  1:7326884/93‑1 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                           <  2:2864363/93‑1 (MQ=255)

                    tggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgc                                                                        >  1:4419062/1‑93 (MQ=255)

                      gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGGCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcgc                                                                      <  1:9018263/93‑1 (MQ=255)

                           ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                                 <  1:6025039/93‑1 (MQ=255)

                           ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                                 <  1:2792336/93‑1 (MQ=255)

                            gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                <  2:7962399/93‑1 (MQ=255)

                             ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                               <  1:2625148/93‑1 (MQ=255)

                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  1:1847021/93‑1 (MQ=255)

                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  2:2283790/93‑1 (MQ=255)

                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  1:7489020/93‑1 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                             >  2:5882384/1‑93 (MQ=255)

                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           >  2:1382034/1‑93 (MQ=255)

                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           <  2:5268970/93‑1 (MQ=255)

                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           <  2:3038703/93‑1 (MQ=255)

                                  ttGTGGCGGTAGCTGCCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                          <  1:165624/93‑1 (MQ=255)

                                    gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                        <  2:9877244/93‑1 (MQ=255)

                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                      <  1:6575304/93‑1 (MQ=255)

                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                      <  2:4704805/93‑1 (MQ=255)

                                       gCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAg                                                     <  2:5702156/93‑1 (MQ=255)

                                        cGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAgg                                                    >  2:179705/1‑93 (MQ=255)

                                          gTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCt                                                  >  1:1478335/1‑93 (MQ=255)

                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                 <  1:2129908/93‑1 (MQ=255)

                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                 <  1:5588256/93‑1 (MQ=255)

                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                 >  1:2337635/1‑93 (MQ=255)

                                            aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                >  1:4134663/1‑93 (MQ=255)

                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                              >  1:1044754/1‑93 (MQ=255)

                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                             <  1:9522744/93‑1 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                            >  2:1363697/1‑93 (MQ=255)

                                                 gttgAGAGCCGGGCTGGATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  2:1412213/91‑1 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  2:10002704/93‑1 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          <  1:9280655/93‑1 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          >  2:8146952/1‑93 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          <  2:1561395/93‑1 (MQ=255)

                                                   tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagagg                                         >  2:1182147/1‑92 (MQ=255)

                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                        <  1:6370242/93‑1 (MQ=255)

                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                        >  2:7184682/1‑93 (MQ=255)

                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                      >  2:4356063/1‑93 (MQ=255)

                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                      >  2:6618406/1‑93 (MQ=255)

                                                       acgcGGACTGTATGACCGCGAAGGCGGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGCCTGGGCAGAGCGCGa                                     <  2:125200/90‑1 (MQ=255)

                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  1:3874358/93‑1 (MQ=255)

                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  2:1987873/93‑1 (MQ=255)

                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  1:1377125/93‑1 (MQ=255)

                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  2:577853/93‑1 (MQ=255)

                                                          ggggTTGTATGCCCGAGAGGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                  <  2:9634468/92‑1 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                  <  2:485733/93‑1 (MQ=255)

                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                                 <  2:3371507/93‑1 (MQ=255)

                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                                 <  2:3709418/93‑1 (MQ=255)

                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatattga                               >  1:453525/1‑90 (MQ=255)

                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                               <  2:7797608/93‑1 (MQ=255)

                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                               <  1:5428716/93‑1 (MQ=255)

                                                                gTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGa                            <  2:263986/93‑1 (MQ=255)

                                                                gTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGa                            >  1:5784110/1‑93 (MQ=255)

                                                                 tATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGAGATGTAGCAGaa                           >  2:915841/1‑93 (MQ=255)

                                                                  aTGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                          >  1:6970193/1‑93 (MQ=255)

                                                                   tGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACt                         <  1:619842/93‑1 (MQ=255)

                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                       >  1:3029494/1‑93 (MQ=255)

                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                       <  2:3542707/93‑1 (MQ=255)

                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGAAGAACTGTGGc                    <  2:6549455/93‑1 (MQ=255)

                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAAGGCAGAACTGTGGc                    >  1:8695922/1‑93 (MQ=255)

                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGACAGCGCGATATGGCAGAACTGTGGCaa                  >  1:2281841/1‑93 (MQ=255)

                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                  <  1:5002207/93‑1 (MQ=255)

                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                  <  1:126597/93‑1 (MQ=255)

                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                 >  1:3819643/1‑93 (MQ=255)

                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                 >  1:8557737/1‑93 (MQ=255)

                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg                >  2:6824015/1‑93 (MQ=255)

                                                                              gCCGGGCGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGCGGTATGGCAGAAATGTGGCAAtgct              >  2:4003988/1‑93 (MQ=255)

                                                                              gCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct              <  2:873271/93‑1 (MQ=255)

                                                                                 ggggggCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa           <  1:7183847/93‑1 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa           <  1:9823503/93‑1 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa           >  1:4588827/1‑93 (MQ=255)

                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGGTATGGCAGGACTGTGGCAATGCTGCAt          >  1:2405161/1‑93 (MQ=255)

                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          >  2:2599399/1‑93 (MQ=255)

                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          >  1:8159156/1‑93 (MQ=255)

                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          >  1:7866974/1‑93 (MQ=255)

                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          <  2:5068756/93‑1 (MQ=255)

                                                                                   gAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATg         <  2:247907/93‑1 (MQ=255)

                                                                                   gAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATg         <  2:6412796/93‑1 (MQ=255)

                                                                                    aggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg        <  1:6755213/93‑1 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       <  2:8424912/93‑1 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  2:7652088/1‑93 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  2:7544255/1‑93 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  2:6330306/1‑93 (MQ=255)

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA  >  NC_000913/3744706‑3744888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`