BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,744,958	G→A	G82E (GGA→GAA)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,744,958	0	G	A	99.6%	1819.3 / NA	500	G82E (GGA→GAA)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (255/242); minor base C (2/0); total (258/242)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 521 total aligned reads displayed.

GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC  >  NC_000913/3744868‑3745047

ggCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaa                                                                                         <  1:4121851/93‑1 (MQ=255)

 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaaa                                                                                        >  2:729875/1‑93 (MQ=255)

 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaaa                                                                                        <  2:5220775/93‑1 (MQ=255)

   aaTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAgg                                                                                      >  1:530102/1‑93 (MQ=255)

      gctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTGTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  2:10046809/93‑1 (MQ=255)

      gctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  1:6145448/93‑1 (MQ=255)

      gcggcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  2:657426/90‑1 (MQ=255)

       ctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCtt                                                                                  <  2:5895725/93‑1 (MQ=255)

         gcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGt                                                                                <  2:9801599/93‑1 (MQ=255)

          cATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtt                                                                               <  2:3229973/93‑1 (MQ=255)

           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              >  2:9044030/1‑93 (MQ=255)

           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              >  1:6602531/1‑93 (MQ=255)

           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              <  2:563599/93‑1 (MQ=255)

            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             <  1:7689064/93‑1 (MQ=255)

            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             >  1:1365248/1‑93 (MQ=255)

            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             >  2:4094733/1‑93 (MQ=255)

             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  1:6384138/93‑1 (MQ=255)

             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  1:2040872/93‑1 (MQ=255)

              gCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTg                                                                           >  1:1831736/1‑93 (MQ=255)

              gCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTg                                                                           >  2:6145672/1‑93 (MQ=255)

               cTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGc                                                                          >  2:2810500/1‑93 (MQ=255)

                tGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCt                                                                         >  1:8527631/1‑93 (MQ=255)

                 gTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTg                                                                        <  2:7865886/93‑1 (MQ=255)

                  tCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTgg                                                                       >  1:3038009/1‑93 (MQ=255)

                   cATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGa                                                                      <  1:8361795/93‑1 (MQ=255)

                   cATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGa                                                                      <  2:6927585/93‑1 (MQ=255)

                    aTTCGCGCCCTGCTTACTCATTCTGGTGTTTGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAt                                                                     >  2:3547501/1‑93 (MQ=255)

                    aTTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAt                                                                     <  1:1917387/93‑1 (MQ=255)

                     ttCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATa                                                                    >  2:4785983/1‑93 (MQ=255)

                      tCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATaa                                                                   <  2:1039856/93‑1 (MQ=255)

                         cgcCCTGCTTGCTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATaaaaa                                                                <  2:7970358/93‑1 (MQ=255)

                          gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               >  2:4298168/1‑93 (MQ=255)

                           cccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACg                                                              >  1:4611417/1‑93 (MQ=255)

                            ccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGACAAGGGCTTGTTTTTGCTGGATAAAAACGa                                                             >  1:10003839/1‑93 (MQ=255)

                             cTGCTTTCTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            <  2:626376/93‑1 (MQ=255)

                             cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            >  2:3695990/1‑93 (MQ=255)

                             cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            >  2:1283542/1‑93 (MQ=255)

                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           <  2:2520408/93‑1 (MQ=255)

                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           <  2:1383609/93‑1 (MQ=255)

                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           >  2:7037385/1‑93 (MQ=255)

                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           <  2:1528971/93‑1 (MQ=255)

                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           >  1:704590/1‑93 (MQ=255)

                               gCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaa                                                          >  2:8897098/1‑93 (MQ=255)

                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGTTGTATAAAAATGACaac                                                         >  1:5071442/1‑92 (MQ=255)

                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaaa                                                         <  2:6059690/93‑1 (MQ=255)

                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaaa                                                         <  1:7440141/93‑1 (MQ=255)

                                 ttACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAc                                                        >  1:7894126/1‑93 (MQ=255)

                                  tACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAcc                                                       >  1:5168199/1‑93 (MQ=255)

                                  tACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAcc                                                       >  2:4465321/1‑93 (MQ=255)

                                   aCTCATTCTGGTGTTAGCGGGGTACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCg                                                      <  1:9166916/93‑1 (MQ=255)

                                      taTTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  1:3174097/92‑1 (MQ=255)

                                      cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  1:871978/93‑1 (MQ=255)

                                      cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  1:10046056/93‑1 (MQ=255)

                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCgg                                                 >  1:8838869/1‑93 (MQ=255)

                                         tCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAACGCTCggg                                                >  1:4030979/1‑93 (MQ=255)

                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              <  1:8146953/93‑1 (MQ=255)

                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              >  2:8464533/1‑93 (MQ=255)

                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              <  2:1881254/93‑1 (MQ=255)

                                            ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAt                                             >  1:2763260/1‑93 (MQ=255)

                                              tgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGc                                           >  2:7515038/1‑93 (MQ=255)

                                              tgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGc                                           <  2:5489143/93‑1 (MQ=255)

                                               gtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCt                                          >  2:5018510/1‑93 (MQ=255)

                                                ttAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTa                                         >  2:9296981/1‑93 (MQ=255)

                                                ttAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTa                                         >  1:1480301/1‑93 (MQ=255)

                                                  aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtt                                       <  1:5732207/93‑1 (MQ=255)

                                                   gCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAttt                                      >  1:5446247/1‑93 (MQ=255)

                                                   gCGGGGAACAAATTGTAGGTATCGGCATCTCCGCACAGGAACAGGGCTTGTTTTTGCTGGATAAAAACGACAATCCGCTCGGGAATGCTAttt                                      <  1:8985288/93‑1 (MQ=255)

                                                     ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAttttt                                    >  2:8553367/1‑92 (MQ=255)

                                                     ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTg                                    <  2:6818072/93‑1 (MQ=255)

                                                      gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGt                                   >  2:8104125/1‑93 (MQ=255)

                                                       ggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTc                                  <  2:2337635/93‑1 (MQ=255)

                                                         aaCAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCt                                <  1:9110004/93‑1 (MQ=255)

                                                           cAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCg                              <  1:7332242/93‑1 (MQ=255)

                                                            aaaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCgg                             >  1:358419/1‑93 (MQ=255)

                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                            <  2:161094/93‑1 (MQ=255)

                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                            >  2:3928581/1‑93 (MQ=255)

                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG‑TTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAc                           >  1:3809677/1‑93 (MQ=255)

                                                               ttGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAcc                          <  1:9758641/93‑1 (MQ=255)

                                                                 gTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  1:1692278/93‑1 (MQ=255)

                                                                 gTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  2:2097544/93‑1 (MQ=255)

                                                                 gTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  1:9557219/93‑1 (MQ=255)

                                                                      tATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGc                   >  1:2221552/1‑93 (MQ=255)

                                                                      tATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGc                   <  2:5326122/93‑1 (MQ=255)

                                                                      tATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGc                   >  1:5938009/1‑93 (MQ=255)

                                                                       aTCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCg                  >  2:9540316/1‑93 (MQ=255)

                                                                        tCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGa                 >  1:6437953/1‑93 (MQ=255)

                                                                         cGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGAt                <  1:4951110/93‑1 (MQ=255)

                                                                          ggCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATg               <  2:7129422/93‑1 (MQ=255)

                                                                           gCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATgg              >  1:4221022/1‑93 (MQ=255)

                                                                             aTCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaa            >  1:8450427/1‑93 (MQ=255)

                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACGAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGGTGGaaa           >  2:8248224/1‑93 (MQ=255)

                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaca           >  1:4347674/1‑91 (MQ=255)

                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  1:5841986/1‑93 (MQ=255)

                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  1:6705944/1‑93 (MQ=255)

                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  2:618703/1‑93 (MQ=255)

                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          <  1:7545057/93‑1 (MQ=255)

                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          >  2:3175233/1‑93 (MQ=255)

                                                                                 ccGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcg        >  2:8162283/1‑93 (MQ=255)

                                                                                   gCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgtt      <  1:2529776/93‑1 (MQ=255)

                                                                                      caGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcgt   <  2:4597513/93‑1 (MQ=255)

GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC  >  NC_000913/3744868‑3745047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`