Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,558,932 2 bp→GC coding (153‑154/831 nt) glpG ← GlpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,558,9320CG99.7% 1083.0 / NA 345V52L (GTG→CTG) glpGGlpG
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (173/171);  total (173/172)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,558,9330GC100.0% 1082.7 / NA 344D51E (GAC→GAG) glpGGlpG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (172/172);  total (172/172)

Only 100 of 365 total aligned reads displayed.

ACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCC  >  NC_000913/3558840‑3559017
                                                                                            ||                                                                                    
     gATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCg                                                                                  >  1:8955372/1‑93 (MQ=255)
          tGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATACCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGt                                                                             <  2:6946199/93‑1 (MQ=255)
             aGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg                                                                          <  2:8211555/93‑1 (MQ=255)
               gCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcgcg                                                                        <  1:6211457/93‑1 (MQ=255)
                 cACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt                                                                      <  1:8296910/93‑1 (MQ=255)
                 cACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCt                                                                      <  2:4843597/93‑1 (MQ=255)
                  aCTGCCGCTATGGCCTGCCTGCCAGCTCGCCGCGAGATAACGCGGATCTGCCGGGTTTTCTAGAAAACGCGCCAGCTCCGCCCGTACGCGCTc                                                                     <  2:9875605/93‑1 (MQ=255)
                   cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg                                                                    >  2:6213211/1‑93 (MQ=255)
                   cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg                                                                    <  2:3996056/93‑1 (MQ=255)
                   cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg                                                                    >  2:3616973/1‑93 (MQ=255)
                   cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCg                                                                    >  2:1690311/1‑93 (MQ=255)
                    tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCgg                                                                   >  2:2784495/1‑93 (MQ=255)
                    tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCgg                                                                   >  1:3912574/1‑93 (MQ=255)
                     gCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGc                                                                  >  1:4105436/1‑93 (MQ=255)
                      ccGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGcc                                                                 >  2:1875080/1‑93 (MQ=255)
                       cGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCt                                                                <  1:1195097/93‑1 (MQ=255)
                       cGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCt                                                                >  2:968600/1‑93 (MQ=255)
                        ggTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTg                                                               >  2:2479000/1‑93 (MQ=255)
                        ggTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTg                                                               >  2:2310051/1‑93 (MQ=255)
                        ggTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTg                                                               >  1:191077/1‑93 (MQ=255)
                        ggTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTg                                                               >  1:3435014/1‑93 (MQ=255)
                        ggTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTg                                                               <  1:2379360/93‑1 (MQ=255)
                         gTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGTTCGGCCTgg                                                              >  2:8757900/1‑93 (MQ=255)
                          tATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg                                                             >  2:1441986/1‑93 (MQ=255)
                          tATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg                                                             <  1:375348/93‑1 (MQ=255)
                          tATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTagg                                                             >  2:1051311/1‑93 (MQ=255)
                           aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGa                                                            >  1:9187017/1‑93 (MQ=255)
                            tGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGAc                                                           <  1:9607313/93‑1 (MQ=255)
                             ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACt                                                          >  1:7785956/1‑93 (MQ=255)
                               cctgcctgCCAGCTCTCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCa                                                        <  2:8561222/93‑1 (MQ=255)
                               cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCa                                                        <  2:9748177/93‑1 (MQ=255)
                                 tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                      >  2:8459633/1‑93 (MQ=255)
                                 tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                      >  1:4607624/1‑93 (MQ=255)
                                 tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                      <  1:9482763/93‑1 (MQ=255)
                                 tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                      >  2:6049627/1‑93 (MQ=255)
                                 tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                      >  2:7819163/1‑93 (MQ=255)
                                 tgcctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATc                                                      <  1:6803719/93‑1 (MQ=255)
                                   cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCg                                                    <  2:8918459/93‑1 (MQ=255)
                                    ctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgc                                                   <  2:577439/93‑1 (MQ=255)
                                    ctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgc                                                   <  1:3147856/93‑1 (MQ=255)
                                    ctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgc                                                   <  2:3484241/93‑1 (MQ=255)
                                       ccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccag                                                >  2:6112206/1‑93 (MQ=255)
                                        cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagc                                               <  1:5709233/93‑1 (MQ=255)
                                        cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagc                                               >  2:5470693/1‑93 (MQ=255)
                                          gCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcca                                             >  2:2619433/1‑93 (MQ=255)
                                          gCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcca                                             >  2:2137955/1‑93 (MQ=255)
                                          gCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagcca                                             >  1:8018878/1‑93 (MQ=255)
                                              gccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACa                                         <  2:4688143/93‑1 (MQ=255)
                                               ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACac                                        >  1:1002921/1‑92 (MQ=255)
                                               ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                        <  2:9233825/93‑1 (MQ=255)
                                                 gccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCg                                      >  1:1588202/1‑93 (MQ=255)
                                                 gccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCg                                      >  2:9153326/1‑93 (MQ=255)
                                                  ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                     <  2:3222179/93‑1 (MQ=255)
                                                  ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                     <  1:9982338/93‑1 (MQ=255)
                                                  ccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                     >  2:6626501/1‑93 (MQ=255)
                                                   cAGATAACGCGGACCTGCCGGGTTTTCGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCt                                    <  1:9037050/93‑1 (MQ=255)
                                                    aGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCtt                                   >  2:5265988/1‑93 (MQ=255)
                                                    aGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCtt                                   >  2:6712848/1‑93 (MQ=255)
                                                    aGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCtt                                   <  2:9442435/93‑1 (MQ=255)
                                                    aGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCtt                                   >  2:2902496/1‑93 (MQ=255)
                                                     gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt                                  >  2:3380811/1‑93 (MQ=255)
                                                     gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt                                  >  2:5053267/1‑93 (MQ=255)
                                                     gATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCttt                                  >  1:2239143/1‑93 (MQ=255)
                                                      aTAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg                                 >  2:3426643/1‑93 (MQ=255)
                                                      aTAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg                                 >  1:6525852/1‑93 (MQ=255)
                                                      aTAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg                                 >  1:4647920/1‑93 (MQ=255)
                                                        aaCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt                               >  1:7942814/1‑93 (MQ=255)
                                                         aCGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt                              <  1:8487261/93‑1 (MQ=255)
                                                          cgcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACCTCGCTTTGGTTa                             >  1:3061817/1‑93 (MQ=255)
                                                          cgcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTa                             <  1:1140445/93‑1 (MQ=255)
                                                          cgcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTa                             <  2:3299645/93‑1 (MQ=255)
                                                            gggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtg                           <  1:3249829/92‑1 (MQ=255)
                                                             ggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgt                          >  1:743607/1‑93 (MQ=255)
                                                             ggATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgt                          >  2:4393708/1‑93 (MQ=255)
                                                               aTCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgttg                        <  2:8872500/93‑1 (MQ=255)
                                                                 cTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCGGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATgttgtt                      >  1:1813946/1‑93 (MQ=255)
                                                                   gCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGa                    >  1:2901128/1‑93 (MQ=255)
                                                                     cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAt                  <  2:8647162/93‑1 (MQ=255)
                                                                     cGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAAt                  <  1:7622462/93‑1 (MQ=255)
                                                                      gggTTTTCGAGAAAACGCGCCAGCTCCGCCTGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATc                 <  2:6290502/93‑1 (MQ=255)
                                                                      gggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATc                 <  1:734650/93‑1 (MQ=255)
                                                                      gggTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATc                 <  1:7728266/93‑1 (MQ=255)
                                                                         ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTg              <  2:3790338/93‑1 (MQ=255)
                                                                         ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTg              <  1:1350713/93‑1 (MQ=255)
                                                                         ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTg              >  2:1584127/1‑93 (MQ=255)
                                                                          tttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa             <  1:4523430/93‑1 (MQ=255)
                                                                          tttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa             <  1:5307432/93‑1 (MQ=255)
                                                                           ttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAg            >  2:8021863/1‑93 (MQ=255)
                                                                             cGAGAAACCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa          <  2:7462214/93‑1 (MQ=255)
                                                                             cGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa          <  1:8711592/93‑1 (MQ=255)
                                                                                gaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATaa       <  1:8809317/93‑1 (MQ=255)
                                                                                gaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATaa       <  1:6925380/93‑1 (MQ=255)
                                                                                 aacaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAac      <  1:3694586/90‑1 (MQ=255)
                                                                                 aaaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAac      <  2:4549786/93‑1 (MQ=255)
                                                                                 aaaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAac      >  1:4279749/1‑93 (MQ=255)
                                                                                   aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGCGGATAacac    >  1:476165/1‑93 (MQ=255)
                                                                                   aaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAacac    >  1:7554414/1‑93 (MQ=255)
                                                                                     cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc  <  2:7346981/93‑1 (MQ=255)
                                                                                     cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc  <  1:2087181/93‑1 (MQ=255)
                                                                                     cgcgCCAGCTCCGCCCGTACGCGCGCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc  <  1:9791024/93‑1 (MQ=255)
                                                                                            ||                                                                                    
ACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCACGTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCC  >  NC_000913/3558840‑3559017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑