BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,055	Δ1 bp	coding (509/1458 nt)	ascF →	PTS system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,055	0	G	.	99.6%	1027.7 / ‑3.3	225	coding (509/1458 nt)	ascF	PTS system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (107/117); minor base A (1/0); total (108/117)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGC  >  NC_000913/2837969‑2838142

aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaa                                                                                   >  2:299974/1‑93 (MQ=255)

 aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaaa                                                                                  <  1:3923057/93‑1 (MQ=255)

  aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAt                                                                                 >  2:6177570/1‑93 (MQ=255)

  aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAt                                                                                 <  2:4545684/93‑1 (MQ=255)

   aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAtt                                                                                >  2:445012/1‑93 (MQ=255)

   aGGATCGCAGACCTTACCCATTCTGATTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAtt                                                                                <  2:5811565/93‑1 (MQ=255)

      aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                                             <  1:889845/93‑1 (MQ=255)

       tCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaaa                                                                            >  2:646154/1‑93 (MQ=255)

           cGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCa                                                                        <  1:200287/93‑1 (MQ=255)

            gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       <  2:5956025/93‑1 (MQ=255)

            gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                       >  2:4022762/1‑93 (MQ=255)

             aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                                      <  2:2789203/93‑1 (MQ=255)

              ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                                     >  1:4329596/1‑93 (MQ=255)

              ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                                     <  2:3850271/93‑1 (MQ=255)

              ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                                     <  2:3118153/93‑1 (MQ=255)

               cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    >  1:825379/1‑93 (MQ=255)

               cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    >  2:53116/1‑93 (MQ=255)

               cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    >  2:2327754/1‑93 (MQ=255)

               cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                    >  2:850529/1‑93 (MQ=255)

                ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                   <  1:5156375/93‑1 (MQ=255)

                ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                   <  1:2743108/93‑1 (MQ=255)

                ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCATGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                   >  1:3380738/1‑93 (MQ=255)

                ttAACCATGCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                   <  2:4726800/93‑1 (MQ=255)

                  aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                                 <  2:698334/93‑1 (MQ=255)

                  aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                                 >  1:1828796/1‑93 (MQ=255)

                    ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                               >  1:4018944/1‑93 (MQ=255)

                     cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                              <  1:3884705/93‑1 (MQ=255)

                     cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                              >  2:4856221/1‑93 (MQ=255)

                      aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                             <  1:986304/93‑1 (MQ=255)

                      aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                             <  2:3504252/93‑1 (MQ=255)

                       ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                                            >  1:2540006/1‑93 (MQ=255)

                       ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                                            <  2:4194793/93‑1 (MQ=255)

                        tccgAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                           >  2:2121938/4‑93 (MQ=255)

                        tCTGAATGTGATTGGTGACGGTGCTTTCTTCCTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                           >  1:714759/1‑93 (MQ=255)

                          tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                                         >  1:520099/1‑93 (MQ=255)

                          tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGa                                                         >  1:4796980/1‑93 (MQ=255)

                            aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                                       >  1:2709729/1‑93 (MQ=255)

                            aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                                       >  1:2587263/1‑93 (MQ=255)

                             aTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTg                                                      >  1:235655/1‑93 (MQ=255)

                                tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                                   <  2:5875925/93‑1 (MQ=255)

                                tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                                   <  2:3162488/93‑1 (MQ=255)

                                ggATTGGTGACGGTGCTTTCTTCTTCTTGCCGCTGATGGTCGCGGCTTCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                                   >  2:2093732/2‑93 (MQ=255)

                                    tGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgc                                               >  1:1322070/1‑93 (MQ=255)

                                     ggTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgcg                                              <  2:2206746/93‑1 (MQ=255)

                                      gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgg                                             <  2:874237/93‑1 (MQ=255)

                                         aCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtg                                          <  2:626537/93‑1 (MQ=255)

                                           ggTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                                        >  2:2814413/1‑93 (MQ=255)

                                            gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                                       >  1:5896522/1‑93 (MQ=255)

                                            gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                                       >  2:2833325/1‑93 (MQ=255)

                                             tGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                                      <  1:654599/93‑1 (MQ=255)

                                             tGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                                      >  2:3474649/1‑93 (MQ=255)

                                             tGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                                      <  1:4848150/93‑1 (MQ=255)

                                              gCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg                                     >  2:3315373/1‑93 (MQ=255)

                                                tttCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt                                   >  2:3705888/1‑93 (MQ=255)

                                                 ttcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa                                  >  2:6126929/1‑93 (MQ=255)

                                                  tcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTAc                                 >  2:1173559/1‑93 (MQ=255)

                                                  tcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTAc                                 <  1:4171290/93‑1 (MQ=255)

                                                     tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                              <  1:3474649/93‑1 (MQ=255)

                                                     tcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                              <  2:5561020/93‑1 (MQ=255)

                                                      cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATcc                             >  1:3189608/1‑93 (MQ=255)

                                                      cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATcc                             >  1:6120808/1‑93 (MQ=255)

                                                      cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATcc                             >  2:3608667/1‑93 (MQ=255)

                                                       ttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCg                            >  2:443406/1‑93 (MQ=255)

                                                       ttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCg                            <  1:6265601/93‑1 (MQ=255)

                                                         ccTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                          >  1:5651278/1‑93 (MQ=255)

                                                           tGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                        <  2:5734049/93‑1 (MQ=255)

                                                              cGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa                     >  1:5544623/1‑93 (MQ=255)

                                                               gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt                    >  2:6247308/1‑93 (MQ=255)

                                                               gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCAGGTGTGCTGGTACATCCGAGTTTTAt                    >  2:5094162/1‑93 (MQ=255)

                                                                 tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                  <  1:5748027/93‑1 (MQ=255)

                                                                 tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                  <  2:5199132/93‑1 (MQ=255)

                                                                 tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                  <  2:1763020/93‑1 (MQ=255)

                                                                 tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                  <  2:82070/93‑1 (MQ=255)

                                                                  gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa                 >  1:6056176/1‑93 (MQ=255)

                                                                   aTGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa                >  2:577277/1‑93 (MQ=255)

                                                                   aTGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa                >  2:240830/1‑93 (MQ=255)

                                                                     ggTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACt              >  1:4548843/1‑93 (MQ=255)

                                                                     ggTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACt              >  1:4056827/1‑93 (MQ=255)

                                                                        cgcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGAt           <  1:5453306/89‑1 (MQ=255)

                                                                         gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          >  2:182845/4‑93 (MQ=255)

                                                                         gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          <  1:3787022/90‑1 (MQ=255)

                                                                         gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          <  1:3070088/90‑1 (MQ=255)

                                                                         gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          <  1:4280883/90‑1 (MQ=255)

                                                                         gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg          <  1:5395434/90‑1 (MQ=255)

                                                                          cggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATgg         <  1:5014/91‑1 (MQ=255)

                                                                          cggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATgg         <  1:1188435/91‑1 (MQ=255)

                                                                          cggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATgg         <  1:623165/91‑1 (MQ=255)

                                                                          cggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATgg         <  1:1010791/91‑1 (MQ=255)

                                                                           ggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGc        <  1:412316/92‑1 (MQ=255)

                                                                           ggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGc        <  2:1938899/92‑1 (MQ=255)

                                                                            gcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCg       <  2:1300316/93‑1 (MQ=255)

                                                                             cATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGa      >  1:3524591/1‑93 (MQ=255)

                                                                               tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    >  2:4669903/1‑93 (MQ=255)

                                                                               tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    <  1:2060609/93‑1 (MQ=255)

                                                                               tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    <  2:3036047/93‑1 (MQ=255)

                                                                               tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    >  1:5786584/1‑93 (MQ=255)

                                                                               tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa    <  2:3401614/93‑1 (MQ=255)

                                                                                cTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAg   <  1:3565830/93‑1 (MQ=255)

                                                                                cTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAg   <  1:1150491/93‑1 (MQ=255)

                                                                                 tGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc  <  1:2265892/93‑1 (MQ=255)

AAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGC  >  NC_000913/2837969‑2838142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`