BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,360	+G	coding (166/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,358	1	.	G	99.5%	648.2 / ‑3.9	203	coding (164/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (107/95); total (108/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.84e-01

Only 100 of 205 total aligned reads displayed.

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGcc                                                                   <  1:1076592/93‑1 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGcc                                                                   <  1:5981087/93‑1 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGcc                                                                   >  1:4973440/1‑93 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGcc                                                                   >  1:4657486/1‑93 (MQ=255)

tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGcc                                                                   <  2:3142478/93‑1 (MQ=255)

   tgacgcTTGCTGTTGGATTAAAGCAAGTAATGCCGCTCGATAATGTTATGGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTg                                                                <  2:2347609/90‑1 (MQ=255)

   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTg                                                                >  2:4930959/1‑93 (MQ=255)

    ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGc                                                               >  2:5990426/1‑93 (MQ=255)

     gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGcc                                                              >  2:2639925/1‑93 (MQ=255)

     gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGcc                                                              <  2:2712239/93‑1 (MQ=255)

       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCt                                                            <  1:3007706/93‑1 (MQ=255)

       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCt                                                            <  1:3779375/93‑1 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTg                                                           >  2:3674252/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTg                                                           >  1:2760924/1‑93 (MQ=255)

        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTg                                                           <  2:1909449/93‑1 (MQ=255)

         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGa                                                          >  2:3793616/1‑93 (MQ=255)

         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGa                                                          <  2:2080042/93‑1 (MQ=255)

         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGa                                                          <  1:5496086/93‑1 (MQ=255)

         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGa                                                          >  2:4733843/1‑93 (MQ=255)

            cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCa                                                       <  2:5750149/93‑1 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCAt                                                      >  2:4137190/1‑93 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCAt                                                      >  2:2552077/1‑93 (MQ=255)

             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCAt                                                      >  1:1891719/1‑93 (MQ=255)

              tttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATg                                                     <  2:4115029/92‑1 (MQ=255)

              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATg                                                     >  2:3988264/1‑93 (MQ=255)

                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATggg                                                   >  1:2565364/1‑93 (MQ=255)

                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGt                                                  >  1:4992565/1‑93 (MQ=255)

                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCTTCAAAGCCGGGGCC‑GC‑C‑GGGCGGCTCGGTAGCCTTGCCCTGTTCTTGGGtt                                                 >  1:5549170/1‑93 (MQ=255)

                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGtt                                                 >  1:3294092/1‑93 (MQ=255)

                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGtt                                                 <  2:1870636/93‑1 (MQ=255)

                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGtt                                                 <  1:3598747/93‑1 (MQ=255)

                   aTTANTNCAAGGAATGCCGCTGGATAAAGTNATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGttt                                                <  1:76558/93‑1 (MQ=255)

                   aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGttt                                                <  1:1749122/93‑1 (MQ=255)

                   aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGttt                                                <  2:1600147/93‑1 (MQ=255)

                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  1:3131629/1‑93 (MQ=255)

                     tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              >  1:1610063/1‑93 (MQ=255)

                      aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGGCGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTgg                                             <  1:3621974/93‑1 (MQ=255)

                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTggg                                            >  1:806758/1‑92 (MQ=255)

                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  2:3365553/1‑93 (MQ=255)

                       aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  1:942426/1‑93 (MQ=255)

                        tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           >  1:4074710/1‑93 (MQ=255)

                         gCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                          >  2:2688101/1‑93 (MQ=255)

                         gCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                          <  2:6434/93‑1 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         <  1:4773782/93‑1 (MQ=255)

                          cAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GGAG‑GGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                         >  2:5572493/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  1:2406792/1‑93 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  1:2792620/93‑1 (MQ=255)

                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        >  1:1816064/1‑93 (MQ=255)

                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  2:1186294/93‑1 (MQ=255)

                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:2183521/93‑1 (MQ=255)

                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:4526932/93‑1 (MQ=255)

                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    >  1:2506440/1‑93 (MQ=255)

                               aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  2:4400861/93‑1 (MQ=255)

                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  2:3293258/93‑1 (MQ=255)

                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  1:5672610/1‑93 (MQ=255)

                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  1:64628/93‑1 (MQ=255)

                                  gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGTCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  1:143511/1‑93 (MQ=255)

                                  gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGGCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:2257439/1‑93 (MQ=255)

                                      cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             >  2:1266760/1‑93 (MQ=255)

                                      cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             >  1:2578855/1‑93 (MQ=255)

                                      cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             >  1:2289332/1‑93 (MQ=255)

                                       tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  1:1196779/93‑1 (MQ=255)

                                        tgATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:6209459/92‑1 (MQ=255)

                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  2:4768755/93‑1 (MQ=255)

                                         gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          >  2:3670238/1‑93 (MQ=255)

                                          aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                         >  1:1335014/1‑93 (MQ=255)

                                          aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                         <  2:5816294/93‑1 (MQ=255)

                                          aTAAAGTTATTGGCTCCATCAAAGCCGGGGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTCTTGGCGCAATGCTGGGCAAAATGc                         >  1:2908612/1‑93 (MQ=255)

                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCt                        >  1:139838/1‑93 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       <  2:2973931/93‑1 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       >  2:1979545/1‑93 (MQ=255)

                                            aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       <  2:344807/93‑1 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  2:3191640/93‑1 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  2:1822689/93‑1 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  2:1409198/1‑93 (MQ=255)

                                             aaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  1:5901200/93‑1 (MQ=255)

                                              aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  1:1000206/93‑1 (MQ=255)

                                               gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    >  2:4262111/1‑93 (MQ=255)

                                               gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    >  1:113338/1‑93 (MQ=255)

                                               gTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                    <  2:4995182/93‑1 (MQ=255)

                                               atgaTTGTCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATCCTGGCa                    <  2:3403654/90‑1 (MQ=255)

                                                ttATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCCTGCCCTGATCATGGGGTTTTGCGCAATGCTGGGCAAAATGCTGGCAg                   >  2:5706274/1‑93 (MQ=255)

                                                 tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  >  1:3462392/1‑93 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:5769067/1‑93 (MQ=255)

                                                   ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  2:603693/1‑93 (MQ=255)

                                                    tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               <  2:3423295/93‑1 (MQ=255)

                                                    tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               <  2:250730/93‑1 (MQ=255)

                                                      gCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  2:3557774/93‑1 (MQ=255)

                                                       cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            >  1:5155859/1‑93 (MQ=255)

                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  2:4891162/1‑93 (MQ=255)

                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:4419224/93‑1 (MQ=255)

                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:5208980/93‑1 (MQ=255)

                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  1:454627/1‑93 (MQ=255)

                                                            tCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:6154799/1‑93 (MQ=255)

                                                            tCAAAGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  2:2774890/1‑93 (MQ=255)

                                                              aaaGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCACTGCTGGGCAAAATGCTGGCAGAATGCGGTGGCGca     >  1:5813766/1‑93 (MQ=255)

                                                               aaGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATTGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  2:5509030/1‑93 (MQ=255)

                                                               aaGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:2025707/1‑93 (MQ=255)

                                                                aGCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  1:4347354/93‑1 (MQ=255)

                                                                 gCCGGTGTCGG‑‑CGGGACGC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  <  2:3846686/93‑1 (MQ=255)

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`