BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,198	(C)_5→6	coding (1041/1203 nt)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,193	1	.	C	100.0%	643.3 / NA	197	D349G (GAT→GGT)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (127/70); total (127/70)

Only 100 of 219 total aligned reads displayed.

GACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG  >  NC_000913/3897101‑3897280

gACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑t                                                                                          >  2:2059498/1‑93 (MQ=255)

    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑cctc                                                                                     >  1:3733573/1‑91 (MQ=255)

    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑cccc                                                                                     >  1:3420242/1‑93 (MQ=255)

     ggATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccc                                                                                    <  2:3832911/93‑1 (MQ=255)

     ggATATTCCTCGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccc                                                                                    <  1:3496740/93‑1 (MQ=255)

        tatTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccac                                                                                 <  2:1946410/93‑4 (MQ=255)

        tatTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccac                                                                                 >  1:2121573/1‑90 (MQ=255)

         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccaca                                                                                <  2:120441/93‑5 (MQ=255)

         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccaca                                                                                >  1:5189432/1‑89 (MQ=255)

           tCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAc                                                                               >  1:2644748/1‑93 (MQ=255)

           tCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAc                                                                               <  1:5204737/93‑1 (MQ=255)

            ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAcc                                                                              >  1:2732929/1‑93 (MQ=255)

            ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAcc                                                                              >  2:3941276/1‑93 (MQ=255)

            ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAcc                                                                              >  1:5416528/1‑93 (MQ=255)

             cTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACca                                                                             >  1:1566261/1‑93 (MQ=255)

              tGGTAATCAAAATTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACcac                                                                            <  2:3338645/93‑1 (MQ=255)

               ggTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACcaca                                                                           <  2:5240484/93‑1 (MQ=255)

                gTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAg                                                                          <  2:1763872/93‑1 (MQ=255)

                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGt                                                                         >  2:4080225/1‑93 (MQ=255)

                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGt                                                                         >  1:3148446/1‑93 (MQ=255)

                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGt                                                                         >  2:2212783/1‑93 (MQ=255)

                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTa                                                                        >  1:2054848/1‑93 (MQ=255)

                   aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTaa                                                                       >  1:1725820/1‑93 (MQ=255)

                   aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTaa                                                                       <  2:4704365/93‑1 (MQ=255)

                     cAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTaaaa                                                                     <  2:3262723/93‑1 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAt                                                                    >  1:4306045/1‑93 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAt                                                                    >  1:4418799/1‑93 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAt                                                                    >  2:6243146/1‑93 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAAGAAAAt                                                                    >  2:4789656/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  1:3909099/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  2:5807566/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  2:5998178/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  1:5722144/1‑93 (MQ=255)

                        aGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTa                                                                  <  1:4234182/93‑1 (MQ=255)

                          ttAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACCACCACCGTAAAATTACg                                                                >  2:5211643/1‑93 (MQ=255)

                           tAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGt                                                               >  2:1885396/1‑93 (MQ=255)

                           tAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGt                                                               <  1:675642/93‑1 (MQ=255)

                            aaTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTa                                                              <  2:3555196/93‑1 (MQ=255)

                                tttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTg                                                          <  2:2695612/93‑1 (MQ=255)

                                  tttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCt                                                        <  2:3157883/93‑1 (MQ=255)

                                  tttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCt                                                        <  2:4328433/93‑1 (MQ=255)

                                   ttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTg                                                       >  2:5589579/1‑93 (MQ=255)

                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                                      <  1:5609051/93‑1 (MQ=255)

                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                                      >  2:2827865/1‑93 (MQ=255)

                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                                      <  1:4328560/93‑1 (MQ=255)

                                     cTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGt                                                     >  2:4288560/1‑93 (MQ=255)

                                      tGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCATAGTAAAATTACGTAGTTGCTGGtt                                                    <  2:4131636/93‑1 (MQ=255)

                                      tGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGtt                                                    <  1:5791035/93‑1 (MQ=255)

                                         tCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAt                                                 >  1:3337305/1‑93 (MQ=255)

                                         tCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAt                                                 >  2:1513315/1‑93 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                <  1:4765806/93‑1 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                >  2:1003209/1‑93 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                <  2:4430009/93‑1 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                <  2:5315232/93‑1 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                >  1:2975320/1‑93 (MQ=255)

                                           aaGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAg                                               <  1:5286277/93‑1 (MQ=255)

                                             gCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCt                                             >  1:1424800/1‑93 (MQ=255)

                                               tCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGc                                           >  1:2691146/1‑93 (MQ=255)

                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACTTCTTTACC‑CCCCCACAACCGCAGTAAAATTACGTAGTTGCTGGTTGATAGCTGcc                                          <  2:5391528/93‑1 (MQ=255)

                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGcc                                          >  2:2521556/1‑93 (MQ=255)

                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGCTGCTGGTTGATAGCTGcc                                          >  2:882343/1‑93 (MQ=255)

                                                  gTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCgg                                        >  2:5859922/1‑93 (MQ=255)

                                                   ttttCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGa                                       >  2:704289/1‑93 (MQ=255)

                                                   ttttCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGa                                       >  1:6025472/1‑93 (MQ=255)

                                                        aGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGtt                                  >  1:17788/1‑93 (MQ=255)

                                                         gCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTc                                 >  1:77785/1‑93 (MQ=255)

                                                         gCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTc                                 <  1:5957564/93‑1 (MQ=255)

                                                          cccGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCa                                >  2:1559794/1‑93 (MQ=255)

                                                           ccGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAg                               >  2:4623381/1‑93 (MQ=255)

                                                             ggCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTc                             >  1:1628786/1‑93 (MQ=255)

                                                              gCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCg                            <  2:2725783/93‑1 (MQ=255)

                                                               cGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGGAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCgg                           <  1:1049220/93‑1 (MQ=255)

                                                                 atatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                         >  2:633083/1‑93 (MQ=255)

                                                                 atatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                         <  2:4214497/93‑1 (MQ=255)

                                                                  tatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc                        >  1:4163884/1‑93 (MQ=255)

                                                                  tatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc                        >  2:174176/1‑93 (MQ=255)

                                                                   atCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACTGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcg                       <  2:5630633/93‑1 (MQ=255)

                                                                   atCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcg                       >  2:5068302/1‑93 (MQ=255)

                                                                    tCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                      >  1:942968/1‑93 (MQ=255)

                                                                    tCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                      >  1:2911583/1‑93 (MQ=255)

                                                                    tCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                      >  1:4043697/1‑93 (MQ=255)

                                                                       ttGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGac                   <  2:5724323/93‑1 (MQ=255)

                                                                       ttGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGGAGGTGCTGGTTGATAGCTGCCGGATCGCTCAGTCGCGCcgcgca                   >  2:63028/1‑91 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt               >  1:2834287/1‑93 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt               >  2:2925304/1‑93 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt               >  2:1075663/1‑93 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGAAACCt               >  1:4888154/1‑93 (MQ=255)

                                                                            ggTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCtt              <  2:3452393/93‑1 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCtta             >  2:1354309/1‑92 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc             >  2:5025063/1‑93 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc             <  2:4807274/93‑1 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc             >  1:3410252/1‑93 (MQ=255)

                                                                                  gACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCg        >  2:3801219/1‑93 (MQ=255)

                                                                                     aTCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTa     >  1:5985348/1‑93 (MQ=255)

                                                                                      tCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAc    >  1:3536102/1‑93 (MQ=255)

                                                                                      tCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAc    >  1:2322631/1‑93 (MQ=255)

                                                                                       cTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACg   <  1:3014156/93‑1 (MQ=255)

                                                                                        tttA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACgg  <  1:5557543/93‑1 (MQ=255)

                                                                                        tttA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACgg  <  2:4381442/93‑1 (MQ=255)

                                                                                        tttA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACgg  >  1:5423013/1‑93 (MQ=255)

GACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG  >  NC_000913/3897101‑3897280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`