BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	92.6%	424.8 / 27.0	150	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (2/9); new base C (39/99); total (42/108)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 189 total aligned reads displayed.

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980197‑3980376

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑t                                                                                           <  2:4012867/93‑1 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑cc                                                                                        >  2:592701/1‑93 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc                                                                                       >  2:764832/1‑93 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGATTCAA‑G‑T‑ccc                                                                                       >  1:4192244/1‑93 (MQ=255)

    attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑c                                                                                     >  2:3855494/1‑93 (MQ=255)

    attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑T‑ccc‑c                                                                                     >  1:2276502/1‑93 (MQ=255)

      taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑ccc                                                                                   <  1:2623366/93‑1 (MQ=255)

       aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑cccc                                                                                  >  1:1687572/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑ccccc                                                                                 >  1:2480416/1‑93 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑cccccc                                                                                <  1:4545163/93‑2 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑cccccc                                                                                >  1:2950558/1‑92 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑cccccct                                                                               <  1:1222921/93‑3 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑T‑ccc‑cccccct                                                                               >  2:362508/1‑91 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑ccc‑cccccctc                                                                              >  1:2380633/1‑90 (MQ=255)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGcaccac                                                                        <  2:4350056/93‑1 (MQ=16)

                   aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACg                                                                       <  1:6049559/93‑1 (MQ=16)

                   aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACg                                                                       <  2:6136281/93‑1 (MQ=16)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGa                                                                      >  2:1851403/1‑93 (MQ=16)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑CCC‑CCCCCTCGCACCACGAc                                                                     <  2:1289233/93‑1 (MQ=34)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGAc                                                                     <  1:3443209/93‑1 (MQ=255)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGAc                                                                     <  2:1956893/93‑1 (MQ=255)

                         aaGGTGCCGGAATTGGTAGACGTTCTAGCTTCAGGTGTTAGTGTCTTTACGGACGTGCGGATTCAA‑GAC‑CCC‑CCCCCTCGCACCACGACTTTa                                                                 <  2:3130106/93‑1 (MQ=14)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTaa                                                                <  2:5351298/93‑1 (MQ=255)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTaa                                                                <  1:1640115/93‑1 (MQ=255)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑TCCCC‑CCCCCTCGCCCCACGACtttta                                                                >  2:3503613/1‑91 (MQ=14)

                           ggcggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTaaa                                                               >  1:2167298/4‑93 (MQ=255)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTaaa                                                               <  1:2713549/93‑1 (MQ=255)

                            gTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAg                                                              <  2:2728476/93‑1 (MQ=255)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGaa                                                            <  1:5947777/93‑1 (MQ=255)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGaa                                                            <  2:4492429/93‑1 (MQ=255)

                              ggCCGAATTGGTAGGCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGaa                                                            <  2:1506000/93‑1 (MQ=14)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAAt                                                           <  1:6291319/93‑1 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAAtt                                                          >  1:4800424/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGTGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGa                                                        <  2:4112847/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGa                                                        <  1:763991/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGa                                                        <  1:4895025/93‑1 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAAc                                                      <  2:1068291/93‑1 (MQ=255)

                                      tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTa                                                    <  1:1106716/93‑1 (MQ=255)

                                       ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaa                                                   <  2:5825736/93‑1 (MQ=255)

                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaa                                                  <  2:5205331/93‑1 (MQ=255)

                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaa                                                  >  1:5072607/1‑93 (MQ=255)

                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑TCCCC‑CCCCCTCGCACCACCACTTTAAAAAATTTAACTaaa                                                  >  1:4917353/1‑93 (MQ=17)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑GCC‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                 <  2:5956303/93‑1 (MQ=255)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                 <  2:3229269/93‑1 (MQ=255)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaa                                                 <  2:4932198/93‑1 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCGA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                <  1:390561/93‑1 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                <  1:651769/93‑1 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                <  1:1471731/93‑1 (MQ=255)

                                           gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                               >  1:4851818/1‑93 (MQ=255)

                                           gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                               <  1:616767/93‑1 (MQ=255)

                                              gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑CCCTCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                            <  1:4429262/93‑1 (MQ=255)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                           <  1:533951/93‑1 (MQ=255)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                           <  1:4341205/93‑1 (MQ=255)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                           <  1:790285/93‑1 (MQ=255)

                                                gcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                          >  1:5935943/1‑93 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTCCGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                         <  1:1256077/93‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                         <  1:1526348/93‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                         >  1:1819976/1‑93 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                        <  2:1967809/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                        <  2:2602720/93‑1 (MQ=255)

                                                    gcatCAGGTTTTAGTGTCCTTACGTACGTGGGGTTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                      <  2:6233320/90‑1 (MQ=25)

                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                      <  2:1651764/93‑1 (MQ=255)

                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                      >  1:5954861/1‑93 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  2:1340022/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  2:2768927/93‑1 (MQ=255)

                                                       tCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTTAAGAATTGAACTAAAAATTCAAAAAGCAGt                                   >  2:2422518/1‑93 (MQ=25)

                                                         aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                 >  2:4168629/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                <  2:1426974/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑TCCCC‑CCCCCTCGCCCCACGACTTTTAAGAATTGAACTAAAAAATTAAAAAGCAGTAtt                                >  1:3556705/1‑93 (MQ=21)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                               <  1:3216528/93‑1 (MQ=255)

                                                             gtTAGTGTCCTTACGGACGTGGGGGTTCAA‑GCC‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                             <  2:4709147/93‑1 (MQ=255)

                                                             gtTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                             >  2:6092432/1‑93 (MQ=255)

                                                             gtTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                             <  1:3947065/93‑1 (MQ=255)

                                                             gtTAGTGTCCTTACGGACGTGGGGGGTCAA‑G‑TCCCC‑CCCCCCCCCCCCCCGCCTTTAAAAAATTAAACTAAAAAATCAAAAAAAAGAAtttgg                             >  2:4570010/1‑91 (MQ=9)

                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                            <  1:912614/93‑1 (MQ=255)

                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                            <  1:2775744/93‑1 (MQ=255)

                                                              ttAGTGGCCTTACGGACGTGGGGTTGCAA‑T‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                            <  1:2680511/93‑1 (MQ=37)

                                                               tAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                           <  2:738375/93‑1 (MQ=255)

                                                                aGTGTCCTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCg                          >  1:2084542/1‑93 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGCTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                         <  2:2837597/93‑1 (MQ=255)

                                                                  tgtCCTTACGGACGTGGGGGTTCAA‑GCC‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                        <  1:1439195/93‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAAGG‑C‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                     <  2:846400/93‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                     >  1:4046791/1‑93 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    <  2:3133473/93‑1 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    >  1:884855/1‑93 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    <  1:5471203/93‑1 (MQ=255)

                                                                       ttACGGACGTGGGGGGTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCCGCGAGTAgcg                   >  2:3742174/1‑93 (MQ=38)

                                                                         aCGGACGTGTGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCa                 <  2:6197667/93‑1 (MQ=255)

                                                                           ggACGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGc               <  1:3467012/93‑1 (MQ=255)

                                                                             aCGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             <  2:5507563/93‑1 (MQ=255)

                                                                             aCGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             <  1:524954/93‑1 (MQ=255)

                                                                              cGTGGGGGTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg            <  2:2380633/93‑1 (MQ=255)

                                                                                 gggggTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa         <  1:1762971/93‑1 (MQ=255)

                                                                                 gggggTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa         <  1:3093702/93‑1 (MQ=255)

                                                                                 gggggTTCAA‑G‑T‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg        >  1:1077670/1‑93 (MQ=255)

                                                                                  ggggTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg        >  1:4215889/1‑93 (MQ=255)

                                                                                   gggTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgc       <  1:2613833/93‑1 (MQ=255)

                                                                                     gTTCAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcgc     >  1:5869982/1‑93 (MQ=255)

                                                                                     gTTCAA‑G‑TACCC‑CCCCCTCGCACCACGACTTTTAAGAATTTAACTAAAAAATCAAAAAACAGTATTTCCGCGAGGAGCGCAGCATGGGAgcgc     >  2:2201633/1‑93 (MQ=255)

                                                                                        cAA‑G‑TCCCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  1:3845063/93‑1 (MQ=255)

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑G‑T‑CCC‑CCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980197‑3980376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`