BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,684	+A	coding (400/501 nt)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,681	1	.	A	100.0%	792.5 / NA	209	Q133K (CAA→AAA)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (113/96); total (113/96)

Only 100 of 231 total aligned reads displayed.

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

gAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac                                                                                          >  2:5101900/1‑93 (MQ=255)

gAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac                                                                                          >  2:367617/1‑93 (MQ=255)

 aGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑a                                                                                        >  1:2503955/1‑93 (MQ=255)

   gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaa                                                                                      <  2:325504/93‑1 (MQ=255)

    tAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaa                                                                                     <  1:2970306/93‑2 (MQ=255)

     aGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaac                                                                                    >  2:2349433/1‑91 (MQ=255)

             tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGAtt                                                                             <  2:4590931/93‑1 (MQ=255)

              gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                            >  2:6204244/1‑93 (MQ=255)

              gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                            <  2:4744360/93‑1 (MQ=255)

              gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                            <  1:5969670/93‑1 (MQ=255)

               aaTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGt                                                                           >  1:6235835/1‑93 (MQ=255)

                aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                          >  1:4108408/1‑93 (MQ=255)

                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTAt                                                                         <  2:5129921/93‑1 (MQ=255)

                  aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                        >  2:3543705/1‑93 (MQ=255)

                  aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                        >  2:5869251/1‑93 (MQ=255)

                       gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTgg                                                                   >  1:5328808/1‑93 (MQ=255)

                       gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTgg                                                                   >  1:134971/1‑93 (MQ=255)

                         aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGa                                                                 >  1:2801211/1‑93 (MQ=255)

                           tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGata                                                               >  1:601115/1‑93 (MQ=255)

                              ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                            >  2:240999/1‑93 (MQ=255)

                              ccATAACAATAAATGAATATAACTCATTCATGGATTCCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                            <  2:4936625/93‑1 (MQ=255)

                               cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCt                                                           >  1:4561985/1‑93 (MQ=255)

                                aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTa                                                          >  2:4601651/1‑93 (MQ=255)

                                  aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACa                                                        <  2:3867839/93‑1 (MQ=255)

                                  aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACa                                                        >  1:4485481/1‑93 (MQ=255)

                                     aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaa                                                     >  1:984677/1‑93 (MQ=255)

                                     aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaa                                                     >  1:2721350/1‑93 (MQ=255)

                                      aTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaa                                                    <  2:6307445/93‑1 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  2:2185641/1‑93 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                   <  1:307915/93‑1 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  1:1847354/1‑93 (MQ=255)

                                        aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                  >  2:753635/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 >  2:4970298/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  2:1540337/93‑1 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  2:4555223/1‑93 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                <  2:5977119/93‑1 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               >  2:5510981/1‑93 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               <  2:572758/93‑1 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               <  2:1110530/93‑1 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              <  2:4857525/93‑1 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              >  1:3680010/1‑93 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  2:2934340/93‑1 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  1:2386505/93‑1 (MQ=255)

                                                ataACTCATTCATGGATTTCAAAAGCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          <  1:5121372/93‑1 (MQ=255)

                                                ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          <  1:5532601/93‑1 (MQ=255)

                                                 taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                         <  1:664631/93‑1 (MQ=255)

                                                 taACTCATTCATGAATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                         <  1:4188947/93‑1 (MQ=255)

                                                  aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                        >  2:5910998/1‑93 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       <  1:5976010/93‑1 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       >  2:2078161/1‑93 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       <  1:3351328/93‑1 (MQ=255)

                                                    cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      <  1:1866143/93‑1 (MQ=255)

                                                    cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      <  1:108390/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  2:4154484/1‑93 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  2:5059901/1‑93 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  2:2220777/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  2:2369758/1‑93 (MQ=255)

                                                      cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACGGAAAAAACAGTCTGACATAc                                    >  2:912880/1‑93 (MQ=255)

                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   >  2:2906048/1‑93 (MQ=255)

                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  2:2619423/93‑1 (MQ=255)

                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  <  2:1879282/93‑1 (MQ=255)

                                                         tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                 >  2:3119343/1‑93 (MQ=255)

                                                          catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                >  1:3278201/1‑93 (MQ=255)

                                                          catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                >  1:1459304/1‑93 (MQ=255)

                                                          catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                >  1:2331991/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               <  1:4043266/93‑1 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               <  1:4620203/93‑1 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  1:4794122/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  2:1576627/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              >  1:4932658/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              >  1:790447/1‑93 (MQ=255)

                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             <  2:5427219/93‑1 (MQ=255)

                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             <  2:2287889/93‑1 (MQ=255)

                                                              gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                            >  1:3629523/1‑93 (MQ=255)

                                                                tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                          <  2:5720683/93‑1 (MQ=255)

                                                                 ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                         <  1:4376926/93‑1 (MQ=255)

                                                                 ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                         >  2:5211980/1‑93 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGTGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                      <  1:3247578/93‑1 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAACAAACAGTCTGACATACCGATAAGGATTata                      >  1:5804475/1‑91 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                      >  2:3845635/1‑93 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                      >  2:2660417/1‑93 (MQ=255)

                                                                         ccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 <  2:5570729/93‑1 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACGGTCTGACATACCGAAAAGGATTAAAGGTtat                <  2:2531554/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               >  2:3154466/1‑93 (MQ=255)

                                                                               aTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa           <  1:1251266/93‑1 (MQ=255)

                                                                                tttCCGTTCGAACAAAACAGATGATTGTATGACTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc          >  2:6132230/1‑93 (MQ=255)

                                                                                tttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc          >  1:569140/1‑93 (MQ=255)

                                                                                 ttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc         <  2:5295190/93‑1 (MQ=255)

                                                                                 ttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc         >  1:508741/1‑93 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        >  2:5323850/1‑93 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        >  2:5251366/1‑93 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        <  2:5788303/93‑1 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        >  1:3233197/1‑93 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        <  1:1983220/93‑1 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        <  2:136921/93‑1 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        >  2:814189/1‑93 (MQ=255)

                                                                                   ccGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTATAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg       >  1:4430332/1‑93 (MQ=255)

                                                                                     gTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc     <  2:1132393/93‑1 (MQ=255)

                                                                                      ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  2:3325845/1‑93 (MQ=255)

                                                                                       tAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa   >  2:4191201/1‑93 (MQ=255)

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`