BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,784	(G)_8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,776	1	.	G	81.5%	379.0 / 95.8	157	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (14/15); new base G (83/45); total (97/60)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

AAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG  >  NC_000913/4603684‑4603863

aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑Ga                                                                                          >  1:5490115/1‑93 (MQ=255)

 aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑g                                                                                        <  2:384998/93‑1 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATTGCCTCGTTTGGT‑GC‑GA‑gg                                                                                       >  2:4483401/1‑93 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gg                                                                                       <  1:2672004/93‑1 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gg                                                                                       >  2:3589733/1‑93 (MQ=255)

   gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggg                                                                                      <  2:1364359/93‑1 (MQ=255)

    acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggg                                                                                     <  1:831695/93‑1 (MQ=255)

     cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggg                                                                                    <  1:1492630/93‑1 (MQ=255)

     cacTAACACCTGAAGCTAGCGCGTCAACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggg                                                                                    <  2:2975262/93‑1 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggg                                                                                  >  1:3957658/1‑93 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggg                                                                                  <  1:2340761/93‑1 (MQ=255)

        tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggggggg                                                                                 <  2:2705531/93‑1 (MQ=255)

        tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggggggg                                                                                 <  1:3104901/93‑1 (MQ=255)

          acacCGGAAGCTGGCGCTTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑gggggggggg                                                                               <  1:5312769/93‑3 (MQ=37)

            acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggggggct                                                                             >  2:4133113/1‑89 (MQ=255)

            acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑ggggggggggat                                                                             >  2:4437447/1‑89 (MQ=255)

                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAc                                                                         >  1:4154859/1‑93 (MQ=255)

                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcc                                                                        >  1:225464/1‑93 (MQ=255)

                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcc                                                                        >  1:1741595/1‑93 (MQ=37)

                   gCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAAcccc                                                                      <  1:1662271/93‑1 (MQ=255)

                   gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAccc                                                                       <  2:1490658/93‑1 (MQ=38)

                    cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcccc                                                                      <  1:4618449/93‑1 (MQ=38)

                    cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAcccc                                                                      >  1:4103334/1‑93 (MQ=255)

                    cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑CC‑GAGGGGGGGGGGCTTGAAcccc                                                                      <  1:2711954/93‑1 (MQ=37)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAccccc                                                                     >  2:2126605/1‑93 (MQ=255)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAccccc                                                                     >  1:1468174/1‑93 (MQ=255)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCa                                                                    <  2:6117242/93‑1 (MQ=255)

                      aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGG‑GC‑GAGGGGGGGGGGCTTGAACCCCCa                                                                    <  2:1531837/93‑1 (MQ=38)

                       gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCAc                                                                   <  1:2299807/93‑1 (MQ=38)

                        cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACg                                                                  >  1:3809420/1‑93 (MQ=255)

                        cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACg                                                                  <  2:5012346/93‑1 (MQ=38)

                         gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACgg                                                                 >  2:5375467/1‑92 (MQ=38)

                         gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGt                                                                 >  1:3551001/1‑93 (MQ=255)

                         ccgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTc                                                                <  2:4676407/92‑1 (MQ=255)

                           gTCTACCAATCCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCGGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTcc                                                               <  2:4797330/93‑1 (MQ=37)

                            tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCg                                                              >  1:5887988/1‑93 (MQ=255)

                               aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGGTTGAACCCCCACGGCCGGaa                                                           >  2:3177/1‑93 (MQ=255)

                               aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTaa                                                           >  2:3071124/1‑93 (MQ=255)

                                ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAg                                                          >  1:4434944/1‑93 (MQ=37)

                                 cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCCCGTCCGTAAGa                                                         >  1:4292414/1‑93 (MQ=38)

                                 cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                        >  2:4303902/1‑93 (MQ=255)

                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                        >  1:6201081/1‑93 (MQ=255)

                                       cGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTa                                                   <  1:5742140/93‑1 (MQ=25)

                                        gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                 >  1:2903099/1‑93 (MQ=255)

                                         ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                 >  2:771054/1‑93 (MQ=255)

                                         ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAAACCCCACGTCCGTAAGCACACTAac                                                 >  1:437991/1‑93 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACCCTaaac                                                >  2:3983699/1‑91 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAaca                                                <  1:4118224/93‑1 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                               >  2:618477/1‑93 (MQ=255)

                                            ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                              >  2:2309491/1‑93 (MQ=255)

                                             cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                             >  1:2817085/1‑93 (MQ=255)

                                              ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACCCTAACACCTg                                            >  2:4151121/1‑93 (MQ=25)

                                               tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGGACCCCCACGTCCGTAAGAACAGTAACACCTGa                                           >  1:5803056/1‑93 (MQ=25)

                                               tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                           <  1:4031510/93‑1 (MQ=255)

                                                cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                         >  1:736682/1‑93 (MQ=255)

                                                  cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                        >  2:1919608/1‑93 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                       >  1:6280307/1‑93 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                      <  2:5159062/93‑1 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                       <  1:6214918/93‑1 (MQ=21)

                                                    caGTCATTTTATTTTTTTTAATATCGCCTGTTTTGGG‑CCGGG‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                      <  2:74259/93‑1 (MQ=12)

                                                    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                      >  1:3203491/1‑93 (MQ=255)

                                                     aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCGAAGCCCGTTACAACACTGACACCTGAAGCTAg                                     >  2:3656576/1‑93 (MQ=21)

                                                      tgtaTCTTACTTTTTTTAATATCGCCTGGTTGGGT‑GC‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                    <  1:3304109/90‑1 (MQ=18)

                                                      gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGGCTGAAACCCCCCGTCCCTAAGAAAAACAAAACCTGAAACTAacg                                   >  2:2881778/1‑93 (MQ=19)

                                                         aTCTAACTTTTTTTGAAATCACCTCGTTTGGG‑GCGGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                 <  2:551122/93‑1 (MQ=17)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                >  1:3719410/1‑93 (MQ=255)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                >  2:5473331/1‑93 (MQ=255)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                >  1:4740751/1‑93 (MQ=255)

                                                           cTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                               >  2:1221919/1‑93 (MQ=255)

                                                            ttACTTTTTTTGATATCGCCTGGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                             <  2:3584949/93‑1 (MQ=255)

                                                            ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                              >  2:5951047/1‑93 (MQ=255)

                                                             tACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                             <  1:1357725/93‑1 (MQ=255)

                                                               cTTTTTTTTGTATCGCCTCGTTTGGA‑GC‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTACCCCCTGAAGCTAGCGCGTCTAcc                           <  2:5670606/93‑1 (MQ=17)

                                                                 ttttttTATAGCGCCTTGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                         <  2:1720055/93‑1 (MQ=14)

                                                                 ttttttGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                         <  1:216990/93‑1 (MQ=255)

                                                                  tttttGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                        <  1:3774028/93‑1 (MQ=255)

                                                                     ttGATAGCTCCTCGTGTGGTGGG‑GC‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                     <  1:2579740/93‑1 (MQ=14)

                                                                      tGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCCCGGCCGTAAGAACACTAACACCTGAACCAAGCGCGTTTACCCATTCCg                    >  2:5879207/1‑93 (MQ=17)

                                                                      tGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc                    >  1:3075200/1‑92 (MQ=17)

                                                                      tGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                    >  2:1208031/1‑93 (MQ=255)

                                                                       gATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                   >  1:512491/1‑93 (MQ=255)

                                                                        atatCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAAGCTAGCGCGTCTACCAATTCCGcc                  >  1:3257421/1‑93 (MQ=17)

                                                                         aagcgccgcGTTTGGT‑GCGGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                 <  2:620456/90‑1 (MQ=14)

                                                                          tgccccTCTTTGGGT‑GG‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                <  2:3459229/89‑1 (MQ=11)

                                                                           tcaccTCGATTGGG‑GCGGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc               <  2:572517/90‑1 (MQ=11)

                                                                            cGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGGTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt              >  1:1246624/1‑93 (MQ=14)

                                                                             tccTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             >  1:98492/2‑93 (MQ=14)

                                                                             gCCTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             >  1:3161271/1‑93 (MQ=255)

                                                                             gCCTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc            >  2:3698497/1‑93 (MQ=255)

                                                                              ccTCGTTTGGT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg           <  2:5139746/93‑1 (MQ=255)

                                                                              ccTCGTTTGGC‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTc            >  1:1074392/1‑93 (MQ=14)

                                                                               cTCGTTTGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg           >  1:5163771/1‑93 (MQ=255)

                                                                                   tttGGT‑GC‑GAGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa       <  2:4907933/93‑1 (MQ=11)

                                                                                   tttGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa       <  1:1920305/93‑1 (MQ=255)

                                                                                   tttGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa       >  1:6115912/1‑93 (MQ=255)

                                                                                    ttGGT‑GCGGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg      <  2:1840166/93‑1 (MQ=11)

                                                                                    ttGGT‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg      <  2:3906980/93‑1 (MQ=255)

                                                                                    ttGGG‑GC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg      <  2:2954527/93‑1 (MQ=11)

                                                                                      ggT‑GC‑GA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc   >  2:4364935/1‑93 (MQ=255)

                                                                                       gT‑GC‑GG‑GTGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg  <  1:483907/93‑1 (MQ=14)

AAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG  >  NC_000913/4603684‑4603863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`