BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,603	(T)_5→6	coding (262/1401 nt)	leuC ←	3‑isopropylmalate isomerase (dehydratase) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,598	1	.	T	100.0%	854.7 / NA	229	N89K (AAC→AAA)	leuC	3‑isopropylmalate isomerase (dehydratase) subunit
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (115/114); total (115/114)

Only 100 of 259 total aligned reads displayed.

GGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑TTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG  >  NC_000913/80506‑80685

ggCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAg                                                                                          <  1:205476/93‑1 (MQ=255)

ggCAAGGTGACGCCCTGTTCCGGCCCCATTACATGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCACATTCTTTGCAg                                                                                          >  2:3516532/1‑93 (MQ=255)

 gCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑t                                                                                        <  1:6066524/93‑1 (MQ=255)

    aGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttt                                                                                     >  1:427839/1‑93 (MQ=255)

    aGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGGGATTCAGGTCATACAGTTCGACTCCAAATTCTTTGCAG‑tttt                                                                                     >  2:6129517/1‑93 (MQ=255)

     ggTGACGCCCTGTTGCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttt                                                                                    >  2:3852111/1‑93 (MQ=255)

     ggTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttt                                                                                    <  1:3854354/93‑1 (MQ=255)

     ggTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttt                                                                                    <  2:1135003/93‑1 (MQ=255)

      gTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttttt                                                                                   <  2:4200312/93‑2 (MQ=255)

      gTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑tttttt                                                                                   <  1:5653583/93‑2 (MQ=255)

         aCGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑ttttttgat                                                                                <  2:1585785/93‑5 (MQ=255)

           gCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCa                                                                               >  2:5293885/1‑93 (MQ=255)

            cccTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAg                                                                              >  1:5147419/1‑93 (MQ=255)

             ccTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGt                                                                             <  1:613124/93‑1 (MQ=255)

              cTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGtt                                                                            >  2:665073/1‑93 (MQ=255)

              cTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGtt                                                                            >  2:3985968/1‑93 (MQ=255)

               tGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTc                                                                           <  1:4329313/93‑1 (MQ=255)

                gTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTcc                                                                          <  2:4945267/93‑1 (MQ=255)

                gTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTcc                                                                          >  1:1880666/1‑93 (MQ=255)

                gTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTcc                                                                          <  1:2685728/93‑1 (MQ=255)

                   tcGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGc                                                                       <  2:1965743/92‑1 (MQ=255)

                   ccGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGc                                                                       >  1:1922221/1‑93 (MQ=255)

                    cGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCa                                                                      >  1:2146362/1‑93 (MQ=255)

                    cGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCa                                                                      <  1:2459675/93‑1 (MQ=255)

                    cGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCa                                                                      <  1:307949/93‑1 (MQ=255)

                     ggCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCAt                                                                     <  2:2427271/93‑1 (MQ=255)

                      gCCCCATTACGTGGACGATCCCCTTATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATc                                                                    <  1:1538384/93‑1 (MQ=255)

                       ccccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCt                                                                   <  2:3306391/93‑1 (MQ=255)

                        cccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTg                                                                  >  2:3252318/1‑93 (MQ=255)

                        cccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTg                                                                  >  2:2583802/1‑93 (MQ=255)

                         ccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTgg                                                                 >  2:1873643/1‑93 (MQ=255)

                         ccATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTgg                                                                 >  1:1980147/1‑93 (MQ=255)

                           atgaCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGAt                                                               <  1:3281728/90‑1 (MQ=255)

                           aTTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGAt                                                               <  1:3754132/93‑1 (MQ=255)

                            ttACGTGGACGATCCCCTGCTACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATa                                                              >  2:6119744/1‑93 (MQ=255)

                            ttACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATa                                                              <  2:4255666/93‑1 (MQ=255)

                            ttACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATa                                                              <  2:5729163/93‑1 (MQ=255)

                            ttACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATa                                                              <  2:5809011/93‑1 (MQ=255)

                             tACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATAc                                                             <  1:4571113/93‑1 (MQ=255)

                               cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGCCGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATAcgc                                                           >  1:4839337/1‑93 (MQ=255)

                               cGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATAcgc                                                           <  2:216936/93‑1 (MQ=255)

                                gTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATAcgcg                                                          >  1:3808417/1‑93 (MQ=255)

                                 tGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACgcgc                                                         <  2:456779/93‑1 (MQ=255)

                                 tGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACgcgc                                                         <  2:1678946/93‑1 (MQ=255)

                                  ggACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGcc                                                        <  1:5087426/93‑1 (MQ=255)

                                       aTCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                   >  2:3624421/1‑93 (MQ=255)

                                       aTCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                   <  1:869929/93‑1 (MQ=255)

                                        tCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCa                                                  >  2:1270702/1‑93 (MQ=255)

                                        tCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCa                                                  <  2:2260831/93‑1 (MQ=255)

                                          cccTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCAcc                                                <  1:1035078/93‑1 (MQ=255)

                                           ccTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCg                                               >  1:765280/1‑93 (MQ=255)

                                           ccTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCg                                               <  1:3665419/93‑1 (MQ=255)

                                           ccTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCg                                               >  1:3554587/1‑93 (MQ=255)

                                            cTGATACGGGTGATTCAGATCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGc                                              <  2:1218254/93‑1 (MQ=255)

                                             tGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCa                                             <  2:5493392/93‑1 (MQ=255)

                                               aTACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAgg                                           <  1:1641795/93‑1 (MQ=255)

                                                tACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGc                                          <  1:5544887/93‑1 (MQ=255)

                                                tACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGc                                          >  1:2283775/1‑93 (MQ=255)

                                                tACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGc                                          >  2:2071178/1‑93 (MQ=255)

                                                   ggggGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAtt                                       >  1:3993904/1‑93 (MQ=255)

                                                   gggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAtt                                       >  1:624766/1‑93 (MQ=255)

                                                   gggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAtt                                       >  1:142085/1‑93 (MQ=255)

                                                    ggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTa                                      >  1:3170920/1‑93 (MQ=255)

                                                    ggTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTa                                      >  2:636059/1‑93 (MQ=255)

                                                     gTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTaa                                     <  1:114704/93‑1 (MQ=255)

                                                     gTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTaa                                     <  1:5400347/93‑1 (MQ=255)

                                                      tGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAAt                                    <  2:4352812/93‑1 (MQ=255)

                                                       gATTCAGGTCATACAGTTCGACGCCAAATTCTTGGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATg                                   <  2:273643/93‑1 (MQ=255)

                                                         ttCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTc                                 <  2:2890170/93‑1 (MQ=255)

                                                         ttCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTc                                 <  2:3964300/93‑1 (MQ=255)

                                                          tCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCt                                <  1:5463325/93‑1 (MQ=255)

                                                          tCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCt                                >  2:6041352/1‑93 (MQ=255)

                                                            aGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCttt                              <  2:4619060/93‑1 (MQ=255)

                                                            aGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCttt                              <  1:988317/93‑1 (MQ=255)

                                                            aGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCttt                              >  2:3093859/1‑93 (MQ=255)

                                                            aGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCttt                              <  1:332211/93‑1 (MQ=255)

                                                             ggTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTg                             <  1:1237485/93‑1 (MQ=255)

                                                              gTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTgg                            >  2:5850040/1‑93 (MQ=255)

                                                               tCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGt                           <  1:288314/93‑1 (MQ=255)

                                                                  tACAGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTg                        >  1:4509182/1‑93 (MQ=255)

                                                                     aGTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGt                     <  1:182091/93‑1 (MQ=255)

                                                                      gTTCGACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTa                    <  1:3620775/93‑1 (MQ=255)

                                                                          gACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAgaga                <  2:1333046/93‑1 (MQ=255)

                                                                          gACGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAgaga                >  2:801709/1‑93 (MQ=255)

                                                                            cGCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACg              >  1:4112281/1‑93 (MQ=255)

                                                                             gCCAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGt             <  1:2635099/93‑1 (MQ=255)

                                                                              ccAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGtt            >  1:4926288/1‑93 (MQ=255)

                                                                              ccAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGtt            <  1:1331028/93‑1 (MQ=255)

                                                                               cAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg           >  2:565591/1‑93 (MQ=255)

                                                                               cAAATTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtg           <  1:4995853/93‑1 (MQ=255)

                                                                                aaaTTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTtgt          <  1:2163852/93‑1 (MQ=255)

                                                                                  aTTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGa        >  2:2224170/1‑93 (MQ=255)

                                                                                  aTTCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGa        >  1:5178277/1‑93 (MQ=255)

                                                                                   ttCTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGAt       >  2:2831313/1‑93 (MQ=255)

                                                                                     cTTTGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATcc     <  2:2100200/93‑1 (MQ=255)

                                                                                      tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    >  1:3356076/1‑93 (MQ=255)

                                                                                      tttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCa    >  2:4075251/1‑93 (MQ=255)

                                                                                       ttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt   >  1:2947681/1‑93 (MQ=255)

                                                                                       ttGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt   >  1:2322958/1‑93 (MQ=255)

                                                                                        tGCAGTTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATg  <  2:3726074/93‑1 (MQ=255)

GGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAG‑TTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATG  >  NC_000913/80506‑80685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`