BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,968	+G	coding (1132/1386 nt)	hofB ←	HofB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,964	1	.	G	98.5%	653.8 / 1.3	204	coding (1136/1386 nt)	hofB	HofB
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (92/109); total (93/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 230 total aligned reads displayed.

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑GGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

gACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGt                                                                                           >  1:45466/1‑93 (MQ=255)

 aCGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g                                                                                         <  2:4040789/93‑1 (MQ=255)

  cGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑gg                                                                                        <  1:1236884/93‑1 (MQ=255)

   gAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg                                                                                       >  2:3540336/1‑93 (MQ=255)

   gAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg                                                                                       >  1:2773527/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg‑g                                                                                     <  2:324743/93‑1 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg‑g                                                                                     >  1:4695334/1‑93 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg‑g                                                                                     >  1:3112896/1‑93 (MQ=255)

      tGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg‑ggc                                                                                   <  2:102061/93‑3 (MQ=255)

       gACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg‑ggca                                                                                  >  1:5412338/1‑90 (MQ=255)

        aCCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑ggg‑ggcag                                                                                 <  2:2275851/93‑5 (MQ=255)

           ggCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAgcgg                                                                               >  2:5131252/1‑93 (MQ=255)

           ggCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAgcgg                                                                               <  2:4105551/93‑1 (MQ=255)

           ggCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAgcgg                                                                               >  1:63312/1‑93 (MQ=255)

             cGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAgcggcg                                                                             >  1:1149988/1‑93 (MQ=255)

             cGTTATGGGCAGAACTTCAAATAAGGCCGTAAGACCATAAAAACCGTGGTAGCAATGTACACAACCGAGTGCCTGCCAGTCGGG‑GCAgcggcg                                                                             >  2:4949935/1‑93 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGa                                                                            <  1:6102459/93‑1 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGAt                                                                           <  2:2231207/93‑1 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGAt                                                                           <  2:2573650/93‑1 (MQ=255)

                  tGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGc                                                                        <  2:5679847/93‑1 (MQ=255)

                  tGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGc                                                                        <  1:3923547/93‑1 (MQ=255)

                    ggCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCa                                                                      <  2:5733637/93‑1 (MQ=255)

                     gCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCAt                                                                     >  1:1840112/1‑93 (MQ=255)

                     gCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCAt                                                                     <  1:2740518/93‑1 (MQ=255)

                      cAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATa                                                                    <  1:1186611/93‑1 (MQ=255)

                      cAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATa                                                                    <  1:5750903/93‑1 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATAc                                                                   <  2:4579135/93‑1 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATAc                                                                   >  1:3773721/1‑93 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACa                                                                  >  1:4615304/1‑93 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACa                                                                  <  1:2865513/93‑1 (MQ=255)

                          aCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACAtt                                                                >  2:361602/1‑93 (MQ=255)

                           cTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTg                                                               <  1:3850513/93‑1 (MQ=255)

                              cAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCt                                                            >  1:921454/1‑93 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTg                                                           <  2:4458406/93‑1 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCAGCGATGGCCATACATTGTCTg                                                           <  1:4995031/93‑1 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTgg                                                          >  1:405782/1‑93 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTgg                                                          >  1:5577004/1‑93 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTgg                                                          >  1:2223973/1‑93 (MQ=255)

                                 aTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGa                                                         <  1:6058683/93‑1 (MQ=255)

                                     ggCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAAtgt                                                     <  2:3832258/93‑1 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  <  2:1496032/93‑1 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  <  2:3408481/93‑1 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGAGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  >  2:5975290/1‑93 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 >  1:2576963/1‑93 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 <  2:2687158/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 <  1:79289/93‑1 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                <  1:352483/93‑1 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                <  2:5020019/93‑1 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                <  1:5932327/93‑1 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                >  1:5219014/1‑93 (MQ=255)

                                           aCGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATgg                                               <  1:4169680/93‑1 (MQ=255)

                                            cGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATggg                                              <  1:1005718/93‑1 (MQ=255)

                                            cGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATggg                                              >  2:1320812/1‑93 (MQ=255)

                                            cGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATATGGATggg                                              <  2:6144829/93‑1 (MQ=255)

                                             gACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGc                                             <  2:3397317/93‑1 (MQ=255)

                                              aCCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCt                                            >  1:245610/1‑93 (MQ=255)

                                                 aTAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                         >  2:1682912/1‑93 (MQ=255)

                                                 aTAAAAACCGTGGTAGCAATGTACACAACCGGGAGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                         >  1:253913/1‑93 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        <  2:3287393/93‑1 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        <  1:1679421/93‑1 (MQ=255)

                                                   aaaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCt                                       <  2:5443270/93‑1 (MQ=255)

                                                   aaaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCt                                       <  2:826893/93‑1 (MQ=255)

                                                    aaaaCCGTGGTAGCAATGTACACAAACGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCtt                                      >  2:1254401/1‑93 (MQ=255)

                                                     aaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtg                                     >  1:2521734/1‑93 (MQ=255)

                                                      agccGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  1:5651230/91‑1 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    >  1:4793656/1‑93 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  2:1556711/93‑1 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  1:3723257/93‑1 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   >  2:2829931/1‑93 (MQ=255)

                                                         cGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgctgc                                 >  1:5318013/1‑93 (MQ=255)

                                                              tAGCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCg                            >  2:4809379/1‑93 (MQ=255)

                                                                gCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGAc                          >  2:761148/1‑93 (MQ=255)

                                                                gCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGAc                          >  1:1607703/1‑93 (MQ=255)

                                                                gCAATGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGAc                          >  2:5918478/1‑93 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  2:5221289/93‑1 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCAATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        >  2:3844499/1‑93 (MQ=255)

                                                                   aTGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAt                       >  1:5375232/1‑93 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      <  2:301763/93‑1 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAGT‑GGGCGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      >  2:5516021/1‑93 (MQ=255)

                                                                     gTACACAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtgt                     >  2:6099739/1‑93 (MQ=255)

                                                                       acacAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTgg                   >  1:5182028/1‑93 (MQ=255)

                                                                       acacAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTgg                   <  2:65452/93‑1 (MQ=255)

                                                                       acacAACCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTgg                   <  2:1937095/93‑1 (MQ=255)

                                                                           aaCCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCaa               >  1:6179044/1‑93 (MQ=255)

                                                                           aaCCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCaa               <  1:1989510/93‑1 (MQ=255)

                                                                            aCCGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCaaa              >  1:1905867/1‑93 (MQ=255)

                                                                              cGGGTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGt            >  2:4708266/1‑93 (MQ=255)

                                                                               gggTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGtt           <  2:2402857/93‑1 (MQ=255)

                                                                                ggTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGttt          <  1:2310616/93‑1 (MQ=255)

                                                                                 gTGCCTGCCAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTg         <  2:3210626/93‑1 (MQ=255)

                                                                                   gcctgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCg       >  2:175082/1‑93 (MQ=255)

                                                                                   gcctgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCg       <  2:1837066/93‑1 (MQ=255)

                                                                                      tgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAc    >  2:4177840/1‑93 (MQ=255)

                                                                                      tgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAc    <  1:1481646/93‑1 (MQ=255)

                                                                                      tgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAc    >  2:4005521/1‑93 (MQ=255)

                                                                                      tgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAc    >  2:3876208/1‑93 (MQ=255)

                                                                                      tgccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAc    >  2:141189/1‑93 (MQ=255)

                                                                                       gccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTAcc   >  1:3278977/1‑93 (MQ=255)

                                                                                        ccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCa  <  1:2620320/93‑1 (MQ=255)

                                                                                        ccAGTGGGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCa  <  1:2415429/93‑1 (MQ=255)

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑GGG‑GCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`