BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,008,480	+A	coding (637/654 nt)	yedK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,008,476	1	.	A	99.4%	652.9 / ‑4.0	175	coding (633/654 nt)	yedK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (76/98); total (76/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 188 total aligned reads displayed.

AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563

aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGt‑t                                                                                          <  1:4309157/93‑1 (MQ=255)

aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGt‑t                                                                                          <  2:2016368/93‑1 (MQ=255)

   ggTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑aaa                                                                                      >  2:861351/1‑93 (MQ=255)

    gTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑aaaa                                                                                     <  1:2278045/93‑1 (MQ=255)

    gTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑aaaa                                                                                     >  1:2994001/1‑93 (MQ=255)

       aagaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑aaaaacc                                                                                  >  2:923783/1‑90 (MQ=255)

        agaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑aaaaacca                                                                                 >  2:5475755/1‑89 (MQ=255)

          aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAgg                                                                                <  1:1544011/93‑1 (MQ=255)

           agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCAGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAggg                                                                               <  2:1069281/93‑1 (MQ=255)

            gCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGgc                                                                              <  2:3278675/93‑1 (MQ=255)

            gCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGgc                                                                              <  1:4513529/93‑1 (MQ=255)

              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGgcgc                                                                            >  2:2579022/1‑93 (MQ=255)

               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGcgcg                                                                           >  1:4572664/1‑93 (MQ=255)

               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGcgcg                                                                           >  2:2429389/1‑93 (MQ=255)

               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGcgcg                                                                           >  2:3129718/1‑93 (MQ=255)

               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGcgcg                                                                           <  1:611476/93‑1 (MQ=255)

                 aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGa                                                                         >  1:4039853/1‑93 (MQ=255)

                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAg                                                                        >  2:2849869/1‑93 (MQ=255)

                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAg                                                                        >  2:4792104/1‑93 (MQ=255)

                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAg                                                                        >  2:6242533/1‑93 (MQ=255)

                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAg                                                                        >  1:1075822/1‑93 (MQ=255)

                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGt                                                                       <  2:1171774/93‑1 (MQ=255)

                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGt                                                                       <  2:4761917/93‑1 (MQ=255)

                        gcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAAtt                                                                  >  2:408513/1‑93 (MQ=255)

                          ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCa                                                                >  2:2978722/1‑93 (MQ=255)

                            ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAAc                                                              <  1:3150120/93‑1 (MQ=255)

                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCt                                                            <  1:4206599/93‑1 (MQ=255)

                                  gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGttt                                                        >  2:3891078/1‑93 (MQ=255)

                                   cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTg                                                       <  2:4631103/93‑1 (MQ=255)

                                   cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTg                                                       >  1:5681286/1‑93 (MQ=255)

                                   cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTg                                                       <  2:5712135/93‑1 (MQ=255)

                                     gtgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                                     >  1:2112113/1‑93 (MQ=255)

                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    <  2:729554/93‑1 (MQ=255)

                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    <  1:4141917/93‑1 (MQ=255)

                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    <  2:3130/93‑1 (MQ=255)

                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    >  2:491140/1‑93 (MQ=255)

                                       gtTCCAGCAAACCAGTTCTCCTGGCACCTGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  1:1058539/93‑1 (MQ=255)

                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   >  2:1517670/1‑93 (MQ=255)

                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  1:4494759/93‑1 (MQ=255)

                                        ttCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAg                                                  >  2:231820/1‑93 (MQ=255)

                                         tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGc                                                 <  2:1854651/93‑1 (MQ=255)

                                          ccAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGcc                                                <  2:1397754/93‑1 (MQ=255)

                                           cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                                               >  1:3214613/1‑93 (MQ=255)

                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              >  2:3484139/1‑93 (MQ=255)

                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              >  1:2318134/1‑93 (MQ=255)

                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              <  1:1369516/93‑1 (MQ=255)

                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGAAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              >  1:4438273/1‑93 (MQ=255)

                                              cAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGga                                            <  1:2780906/93‑1 (MQ=255)

                                                 aCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                         <  2:2936624/93‑1 (MQ=255)

                                                 aCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                         <  2:1698204/93‑1 (MQ=255)

                                                  ccAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatt                                        >  2:46758/1‑93 (MQ=255)

                                                   cAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatta                                       >  1:5810885/1‑93 (MQ=255)

                                                      ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTg                                    <  2:4184833/93‑1 (MQ=255)

                                                       tctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGa                                   <  2:4248994/93‑1 (MQ=255)

                                                        ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAt                                  <  2:652616/93‑1 (MQ=255)

                                                          ccTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATca                                >  1:2639348/1‑93 (MQ=255)

                                                           cTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaa                               >  1:2699740/1‑93 (MQ=255)

                                                            tGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaac                              <  1:5580042/93‑1 (MQ=255)

                                                              gCACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaacaa                            <  2:4601552/93‑1 (MQ=255)

                                                               cACCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAt                           <  2:3221155/93‑1 (MQ=255)

                                                                aCCCGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          >  1:57678/1‑93 (MQ=255)

                                                                 cccGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                         >  1:3653312/1‑93 (MQ=255)

                                                                 cccGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                         >  1:392824/1‑93 (MQ=255)

                                                                 cccGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                         <  2:4867315/93‑1 (MQ=255)

                                                                  ccGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                        >  1:4727871/1‑93 (MQ=255)

                                                                  ccGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                        >  1:3457583/1‑93 (MQ=255)

                                                                   cGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg                       >  1:2353674/1‑93 (MQ=255)

                                                                   cGGTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg                       >  2:3728753/1‑93 (MQ=255)

                                                                    ggTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAGCCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc                      <  2:4313779/93‑1 (MQ=255)

                                                                    ggTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc                      <  1:4887627/93‑1 (MQ=255)

                                                                     gTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                     >  1:5374134/1‑93 (MQ=255)

                                                                     gTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                     >  2:5027218/1‑93 (MQ=255)

                                                                     gTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                     >  2:4480703/1‑93 (MQ=255)

                                                                     gTATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCg                     <  1:1146810/93‑1 (MQ=255)

                                                                      tATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt                    <  1:4226683/93‑1 (MQ=255)

                                                                      tATCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt                    <  1:4159430/93‑1 (MQ=255)

                                                                       aTCGCGCGCGGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc                   <  2:4929466/93‑1 (MQ=255)

                                                                         cgcgcgcgGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCAt                 <  1:3338201/93‑1 (MQ=255)

                                                                          gcgcgcgGTGCGTAATGTGA‑AAAACCAGGGCGCGGAGTTAATTCATCCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATa                <  2:398073/93‑1 (MQ=255)

                                                                           cgcgcgGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATGAACAATACTGCGTCATaa               >  2:5992965/1‑93 (MQ=255)

                                                                               cgGTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  1:973990/93‑1 (MQ=255)

                                                                                ggTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAt          <  1:1704564/93‑1 (MQ=255)

                                                                                ggTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAt          >  1:6283603/1‑93 (MQ=255)

                                                                                 gTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         >  1:3905418/1‑93 (MQ=255)

                                                                                 gTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         <  1:4600368/93‑1 (MQ=255)

                                                                                 gTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         <  2:2364657/93‑1 (MQ=255)

                                                                                 gTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         <  2:1602779/93‑1 (MQ=255)

                                                                                 gTGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         >  1:852694/1‑93 (MQ=255)

                                                                                  tGGGTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtct        >  2:2737337/1‑93 (MQ=255)

                                                                                   gggTAATGT‑TAAAAACTAGGGCGCGGCGGTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATTAAGAATAACGAGTGAAAAGAAAtatc       >  2:4370631/1‑93 (MQ=255)

                                                                                   gggTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       >  2:4976078/1‑93 (MQ=255)

                                                                                   gggTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       >  1:314431/1‑93 (MQ=255)

                                                                                     gTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTa     <  1:5665271/93‑1 (MQ=255)

                                                                                     gTAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTa     <  2:1230642/93‑1 (MQ=255)

                                                                                      tAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAt    <  1:5233278/93‑1 (MQ=255)

                                                                                      tAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAt    <  1:5557399/93‑1 (MQ=255)

                                                                                      tAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAt    <  2:5311697/93‑1 (MQ=255)

                                                                                      tAATGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAt    >  1:370759/1‑93 (MQ=255)

                                                                                       aaTGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTACGTCATAAGAAATCTCTAtt   >  2:5419184/1‑93 (MQ=255)

                                                                                        aTGT‑TAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  >  1:2922016/1‑93 (MQ=255)

AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGT‑T‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`