BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,934	+C	coding (105/1059 nt)	afuC ←	AfuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,930	1	.	C	99.5%	653.2 / ‑3.9	202	coding (109/1059 nt)	afuC	AfuC
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (100/101); total (100/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 219 total aligned reads displayed.

AATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTG‑CCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTT  >  NC_000913/277838‑278017

 aTTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCCCCATTTG‑c                                                                                         >  1:174686/1‑93 (MQ=255)

 aTTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTG‑c                                                                                         >  1:5083283/1‑93 (MQ=255)

  tttGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTG‑cc                                                                                        >  2:3013657/1‑93 (MQ=255)

   ttGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTG‑ccc                                                                                       >  2:2047384/1‑93 (MQ=255)

        cTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTTCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTG‑ccc‑cctgc                                                                                 <  1:5205557/93‑5 (MQ=39)

        cTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTG‑ccc‑cctgc                                                                                 >  1:2171095/1‑89 (MQ=255)

          tCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCgg                                                                                >  1:2798898/1‑93 (MQ=255)

            gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGa                                                                              <  2:4205698/93‑1 (MQ=255)

            gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGa                                                                              <  1:2968368/93‑1 (MQ=255)

             cgcGGTATTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGAt                                                                             <  2:4871059/91‑1 (MQ=38)

             cTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGAt                                                                             <  2:5460460/93‑1 (MQ=39)

             cTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGAt                                                                             <  2:2854718/93‑1 (MQ=255)

             cTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGAt                                                                             <  1:2516313/93‑1 (MQ=255)

              tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATg                                                                            >  2:3149303/1‑93 (MQ=255)

              tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATg                                                                            >  2:5912392/1‑93 (MQ=255)

                ggTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGt                                                                          >  1:2407407/1‑93 (MQ=255)

                ggTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGt                                                                          <  1:4270263/93‑1 (MQ=39)

                 gTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGGGTCACCATTTGCCCC‑CTGCGGGATGGTg                                                                         >  1:1111069/1‑93 (MQ=39)

                  tttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGa                                                                        <  1:100760/93‑1 (MQ=255)

                  tttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGa                                                                        <  1:6058803/93‑1 (MQ=39)

                  tttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGa                                                                        <  1:339292/93‑1 (MQ=39)

                   ttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAg                                                                       <  2:504262/93‑1 (MQ=39)

                   ttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAg                                                                       <  2:639326/93‑1 (MQ=39)

                      tCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATTGTGAGGtt                                                                    <  2:6114113/93‑1 (MQ=38)

                       ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTg                                                                   <  1:6265496/93‑1 (MQ=255)

                       ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTg                                                                   >  1:3749068/1‑93 (MQ=255)

                       ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTg                                                                   >  1:4004703/1‑93 (MQ=255)

                       ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTg                                                                   <  2:4552043/93‑1 (MQ=255)

                       ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTg                                                                   >  1:1993153/1‑93 (MQ=255)

                         aGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGat                                                                 >  2:5867413/1‑93 (MQ=255)

                          gCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGatt                                                                >  1:4148999/1‑92 (MQ=255)

                          gCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGata                                                                <  2:3469852/93‑1 (MQ=255)

                             cGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTg                                                             <  1:1263578/93‑1 (MQ=255)

                              ggCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGt                                                            <  2:5396357/93‑1 (MQ=255)

                               gCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTc                                                           <  1:4888957/93‑1 (MQ=255)

                               gCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTc                                                           <  2:5116021/93‑1 (MQ=255)

                               gCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTc                                                           <  2:2080110/93‑1 (MQ=255)

                                cAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCg                                                          <  2:3484147/93‑1 (MQ=255)

                                 aaCCAGGCGCAAAATAGTGTTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGa                                                         <  2:3526895/93‑1 (MQ=255)

                                  aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGAt                                                        >  2:1995952/1‑93 (MQ=255)

                                  aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGAt                                                        >  1:4462567/1‑93 (MQ=255)

                                    cAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTa                                                      <  1:939398/93‑1 (MQ=255)

                                    cAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTa                                                      >  2:5285472/1‑93 (MQ=255)

                                    cAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTa                                                      >  1:4530165/1‑93 (MQ=255)

                                    cAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTa                                                      >  1:5707421/1‑93 (MQ=255)

                                    cAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTa                                                      <  2:5747567/93‑1 (MQ=255)

                                    cAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTa                                                      >  1:4668159/1‑93 (MQ=255)

                                     aGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTAc                                                     >  2:4168751/1‑93 (MQ=255)

                                      ggCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTAcc                                                    >  2:5150862/1‑93 (MQ=255)

                                        cgcAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGt                                                  <  2:3030952/93‑1 (MQ=255)

                                          cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTAt                                                <  1:5797780/93‑1 (MQ=255)

                                           aaaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTAtt                                               >  2:4439753/1‑93 (MQ=255)

                                           aaaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTAtt                                               >  2:2460910/1‑93 (MQ=255)

                                            aaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTa                                              >  2:3743475/1‑93 (MQ=255)

                                             aaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                             <  2:3923881/93‑1 (MQ=255)

                                             aaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                             <  1:5953757/93‑1 (MQ=255)

                                              aTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACt                                            >  2:2555517/1‑93 (MQ=255)

                                                 gTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGcc                                         >  1:5441472/1‑93 (MQ=255)

                                                 gTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGcc                                         >  1:1713815/1‑93 (MQ=255)

                                                  tGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCa                                        >  1:4128560/1‑93 (MQ=255)

                                                  tGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCa                                        >  2:4802490/1‑93 (MQ=255)

                                                     ttttGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAAt                                     <  2:2986472/93‑1 (MQ=255)

                                                        tGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGt                                  <  2:4130701/93‑1 (MQ=255)

                                                          ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGttt                                >  2:1578646/1‑93 (MQ=255)

                                                            gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                              >  2:1184156/1‑93 (MQ=255)

                                                            gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                              >  2:3180396/1‑93 (MQ=255)

                                                            gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                              >  1:1823858/1‑93 (MQ=255)

                                                              aGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTg                            <  1:2092649/93‑1 (MQ=255)

                                                               gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                           <  1:4222603/93‑1 (MQ=255)

                                                               gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                           >  2:2763494/1‑93 (MQ=255)

                                                               gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                           >  1:1973018/1‑93 (MQ=255)

                                                               gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                           <  2:6152151/93‑1 (MQ=255)

                                                               gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                           >  2:1492172/1‑93 (MQ=255)

                                                                ccGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc                          >  2:5957796/1‑93 (MQ=255)

                                                                 cGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACg                         <  2:1508365/93‑1 (MQ=255)

                                                                 cGGACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACg                         <  1:5826050/93‑1 (MQ=255)

                                                                  ggACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGt                        >  2:2837268/1‑93 (MQ=255)

                                                                   gACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt                       >  2:1668422/1‑93 (MQ=255)

                                                                   gACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt                       >  1:4725691/1‑93 (MQ=255)

                                                                   gACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt                       >  2:4686856/1‑93 (MQ=255)

                                                                   gACGGGCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt                       >  2:6023223/1‑93 (MQ=255)

                                                                      gggCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTgcg                    >  1:5421970/1‑93 (MQ=255)

                                                                        gCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCa                  <  2:207507/93‑1 (MQ=255)

                                                                        gCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCa                  <  2:1012993/93‑1 (MQ=255)

                                                                        gCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCa                  <  2:5803241/93‑1 (MQ=255)

                                                                        gCCGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCa                  <  1:4665968/93‑1 (MQ=255)

                                                                          cGAGCGGCGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGt                <  1:142816/93‑1 (MQ=255)

                                                                            agcggcGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTc              >  1:3102848/1‑93 (MQ=255)

                                                                              cggcGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCaa            <  1:2283213/91‑1 (MQ=255)

                                                                              cggcGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCaa            >  2:2284074/3‑93 (MQ=255)

                                                                              cggcGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCaa            <  1:5569374/91‑1 (MQ=255)

                                                                               ggcGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAAc           <  1:1092624/92‑1 (MQ=255)

                                                                               ggcGTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAAc           <  2:2612224/92‑1 (MQ=255)

                                                                               ggcGTCACCATTTG‑CCCACTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAAc           <  1:3268817/92‑1 (MQ=255)

                                                                                  gTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGaa        >  2:2396500/1‑93 (MQ=255)

                                                                                  gTCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGaa        >  2:3941018/1‑93 (MQ=255)

                                                                                   tCACCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGaaa       >  1:1123168/1‑93 (MQ=255)

                                                                                     aCCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAAtt     <  1:6293852/93‑1 (MQ=255)

                                                                                     aCCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAAtt     >  2:1317688/1‑93 (MQ=255)

                                                                                     aCCATTTGCCCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAAtt     >  2:1808480/1‑93 (MQ=255)

AATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTG‑CCC‑CTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTT  >  NC_000913/277838‑278017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`