BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	300,243	+G	coding (869/957 nt)	yagS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	300,240	1	.	G	100.0%	730.5 / NA	227	H291P (CAT→CCT)	yagS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (132/95); total (132/95)

Only 100 of 251 total aligned reads displayed.

TCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTATGTG  >  NC_000913/300148‑300327

tCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAt                                                                                          <  2:1345088/93‑1 (MQ=255)

  aaaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑gg                                                                                       <  1:4455874/93‑1 (MQ=255)

   aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑ggg                                                                                      >  2:1953059/1‑93 (MQ=255)

    aTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑gggg                                                                                     >  1:224472/1‑92 (MQ=255)

       tCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑ggggcgc                                                                                  <  1:4748784/93‑5 (MQ=255)

         aTGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTg                                                                                 >  2:4574006/1‑93 (MQ=255)

          tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTgg                                                                                <  1:1697577/93‑1 (MQ=255)

          tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTgg                                                                                <  2:5882090/93‑1 (MQ=255)

           gCCTGTGCCCTCGCTTCAGCCATTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGc                                                                               >  1:4035818/1‑93 (MQ=255)

           gCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGc                                                                               >  1:3177221/1‑93 (MQ=255)

              tgtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGaa                                                                            <  1:5666045/93‑1 (MQ=255)

               gtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAAc                                                                           >  1:5059082/1‑93 (MQ=255)

               gtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAAc                                                                           <  2:1465487/93‑1 (MQ=255)

                 gCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAg                                                                         >  2:2199920/1‑93 (MQ=255)

                  cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGc                                                                        >  1:2421698/1‑93 (MQ=255)

                  cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGc                                                                        <  1:4405827/93‑1 (MQ=255)

                   ccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCg                                                                       >  1:1638507/1‑93 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgt                                                                      <  1:2009694/93‑1 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgt                                                                      >  1:3405853/1‑93 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCgg                                                                      >  1:948442/1‑92 (MQ=255)

                        cTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCa                                                                  >  1:709274/1‑93 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCata                                                                <  2:2366060/93‑1 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCata                                                                >  2:4891885/1‑93 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCata                                                                >  2:5117594/1‑93 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCata                                                                <  1:1238750/93‑1 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCata                                                                >  2:6244275/1‑93 (MQ=255)

                            aGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCAtata                                                              <  2:2978095/93‑1 (MQ=255)

                            aGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCAtata                                                              <  1:1797041/93‑1 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             <  1:1135373/93‑1 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  1:849055/1‑93 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  2:599643/1‑93 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             >  1:386867/1‑93 (MQ=255)

                               cAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACgg                                                           >  2:108134/1‑93 (MQ=255)

                                 gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         >  2:2645770/1‑93 (MQ=255)

                                 gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTGTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         <  1:1024948/93‑1 (MQ=255)

                                  tACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCt                                                        <  1:1563020/93‑1 (MQ=255)

                                   aCGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTg                                                       >  2:4503654/1‑93 (MQ=255)

                                    cGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgc                                                      <  2:3900858/93‑1 (MQ=255)

                                      gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    >  2:4208508/1‑93 (MQ=255)

                                       aGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGcc                                                   <  1:4221357/93‑1 (MQ=255)

                                       aGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGcc                                                   >  1:5517762/1‑93 (MQ=255)

                                       aGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGcc                                                   >  1:2270943/1‑93 (MQ=255)

                                          cAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccccc                                                <  2:471293/93‑1 (MQ=255)

                                          cAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGccccc                                                <  2:424217/93‑1 (MQ=255)

                                           aaGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCt                                               <  1:6273574/93‑1 (MQ=255)

                                           aaGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCt                                               <  1:4173434/93‑1 (MQ=255)

                                           aaGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCt                                               >  2:3263606/1‑93 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              >  2:5198962/1‑93 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              >  1:3751790/1‑93 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              >  2:5559668/1‑93 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              <  2:1668795/93‑1 (MQ=255)

                                               gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           <  2:5523321/93‑1 (MQ=255)

                                               gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           >  1:3442071/1‑93 (MQ=255)

                                                ttcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGAt                                          >  1:4915041/1‑93 (MQ=255)

                                                ttcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGAt                                          >  2:868445/1‑93 (MQ=255)

                                                   gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       <  1:3041126/93‑1 (MQ=255)

                                                   gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       >  2:453307/1‑93 (MQ=255)

                                                   gcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGc                                       <  2:4300239/93‑1 (MQ=255)

                                                    cttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCt                                      >  2:3428373/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGCGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  2:6096908/1‑93 (MQ=255)

                                                      tcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGa                                    >  1:1378321/1‑93 (MQ=255)

                                                          cAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCAt                                >  2:5691833/1‑93 (MQ=255)

                                                          cAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCAt                                >  2:3234341/1‑93 (MQ=255)

                                                           aaCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATc                               >  1:5481586/1‑93 (MQ=255)

                                                           aaCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATc                               >  1:3714438/1‑93 (MQ=255)

                                                            aCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCACTGGCGAACGGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATcc                              <  2:3310283/93‑1 (MQ=255)

                                                             cAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCg                             <  1:2496246/93‑1 (MQ=255)

                                                             cAGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCg                             >  2:3851821/1‑93 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            >  2:2285174/1‑93 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            >  2:2567957/1‑93 (MQ=255)

                                                                gAGTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAg                          >  1:20609/1‑93 (MQ=255)

                                                                  gTTTAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGCGCATCCgccgcc                        >  2:2389951/1‑93 (MQ=255)

                                                                   tttAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGCTAGCTGAGCCTCCGCAGCCt                       >  2:4017217/1‑93 (MQ=255)

                                                                     tAAAGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCa                     >  2:53697/1‑93 (MQ=255)

                                                                      aaaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    >  1:5797606/1‑93 (MQ=255)

                                                                      aaaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    >  2:2051657/1‑93 (MQ=255)

                                                                      aaaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCGGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    <  2:2502975/93‑1 (MQ=255)

                                                                      aaaGGTGTTTGCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCaa                    <  1:3988922/93‑1 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAAt                   >  2:2842791/1‑93 (MQ=255)

                                                                        aGGTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATg                  >  2:4321193/1‑93 (MQ=255)

                                                                         ggTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgc                 <  2:2023547/93‑1 (MQ=255)

                                                                         ggTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgc                 <  2:4985907/93‑1 (MQ=255)

                                                                         ggTGTTTTCAGCGGTGGGATGGGGCGCTGGCGAACACCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgc                 <  2:1365995/93‑1 (MQ=255)

                                                                           tgtTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgcgc               <  1:292974/93‑1 (MQ=255)

                                                                            gtTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGcc              <  2:6047300/93‑1 (MQ=255)

                                                                            gtTTTCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGcc              <  1:5260014/93‑1 (MQ=255)

                                                                             ttttCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCa             <  2:2774103/93‑1 (MQ=255)

                                                                             ttttCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCa             >  2:5058420/1‑93 (MQ=255)

                                                                             ttttCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCa             >  1:2592531/1‑93 (MQ=255)

                                                                             ttttCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCa             >  2:5643257/1‑93 (MQ=255)

                                                                              tttCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAg            <  2:706354/93‑1 (MQ=255)

                                                                                tCAGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAggg          <  1:6069055/93‑1 (MQ=255)

                                                                                  aGCGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCt        >  2:1214994/1‑93 (MQ=255)

                                                                                    cGGTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTa      <  2:3663512/93‑1 (MQ=255)

                                                                                     ggTGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAt     >  1:404878/1‑93 (MQ=255)

                                                                                       tGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgt   <  1:6241953/93‑1 (MQ=255)

                                                                                       tGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgt   <  1:5201432/93‑1 (MQ=255)

                                                                                       tGGGATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgt   >  2:3799328/1‑93 (MQ=255)

                                                                                        gggATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgtg  >  2:3154065/1‑93 (MQ=255)

                                                                                        gggATGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgtg  >  1:4654658/1‑93 (MQ=255)

TCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGAT‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTATGTG  >  NC_000913/300148‑300327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`