BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,058	(C)_7→8	coding (585/720 nt)	yggN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,051	1	.	C	96.2%	555.0 / 13.7	182	coding (592/720 nt)	yggN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (103/72); total (107/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 216 total aligned reads displayed.

TTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT  >  NC_000913/3098959‑3099137

 tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c                                                                                        >  2:4545283/1‑93 (MQ=255)

   cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc                                                                                      >  1:1627536/1‑93 (MQ=255)

   cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc                                                                                      >  2:2838126/1‑93 (MQ=255)

   cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc                                                                                      <  2:401375/93‑1 (MQ=255)

     gagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cc                                                                                   >  2:6275723/1‑93 (MQ=255)

       gTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccc                                                                                 <  1:1923666/93‑1 (MQ=255)

        tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑ccccc                                                                                <  1:1232804/93‑2 (MQ=255)

        tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑ccccc                                                                                >  1:4734418/1‑92 (MQ=255)

         cgcaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGATTTGTCCACTCGGTTTGGATTGAGGATTGCC‑G‑‑ccc‑ccccca                                                                               <  2:1066289/91‑3 (MQ=255)

         cacaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cct‑ccccca                                                                               <  2:2806743/93‑3 (MQ=255)

         cacaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑ccccca                                                                               >  2:1100745/1‑91 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  1:4988098/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:1769503/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:44551/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:4582550/93‑4 (MQ=255)

          acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccag                                                                              <  2:5632872/93‑4 (MQ=255)

           caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccc‑cccccagg                                                                             >  2:5547648/1‑89 (MQ=255)

             aCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTTCA‑G‑‑CCCACCACAGGCt                                                                            >  2:5518210/1‑93 (MQ=255)

              cgcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCtt                                                                           >  1:5752762/1‑93 (MQ=255)

               gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTc                                                                          >  2:2443424/1‑93 (MQ=255)

                cgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTcc                                                                         <  2:2267846/93‑1 (MQ=255)

                 ggCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGGTTccc                                                                        >  2:2670827/1‑93 (MQ=255)

                    tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGc                                                                     >  2:1402821/1‑93 (MQ=255)

                    tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGc                                                                     >  1:3937240/1‑93 (MQ=255)

                    tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGc                                                                     >  1:3807433/1‑93 (MQ=255)

                     aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGca                                                                    <  2:3719761/93‑1 (MQ=255)

                     aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGca                                                                    >  1:993865/1‑93 (MQ=255)

                       aaaCATCTTTGCGAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGcaca                                                                  <  2:2451015/93‑1 (MQ=255)

                         aCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACAtt                                                                >  1:4006408/1‑93 (MQ=255)

                         aCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACAtt                                                                <  2:4080371/93‑1 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCCGCACATTc                                                               >  2:1659979/1‑93 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTc                                                               <  2:3247520/93‑1 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTc                                                               <  2:4173141/93‑1 (MQ=255)

                          cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTc                                                               >  1:107327/1‑93 (MQ=255)

                           aTCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCt                                                              >  2:5927377/1‑93 (MQ=255)

                            tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTg                                                             <  1:5075174/93‑1 (MQ=255)

                            tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTg                                                             >  2:5753481/1‑93 (MQ=255)

                              tttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTa                                                           >  2:4051163/1‑93 (MQ=255)

                               ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTaa                                                          >  2:1465896/1‑93 (MQ=255)

                               ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTaa                                                          <  2:507774/93‑1 (MQ=255)

                               ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAAt                                                         <  1:2073774/93‑1 (MQ=255)

                                tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAAt                                                         <  2:1293806/93‑1 (MQ=255)

                                 gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATg                                                        >  1:4832022/1‑93 (MQ=255)

                                  ccAAATTCCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTAAGGATTGCACC‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATgg                                                       <  1:4194735/93‑1 (MQ=255)

                                  ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATgg                                                       <  1:5878293/93‑1 (MQ=255)

                                  ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATgg                                                       >  1:2795970/1‑93 (MQ=255)

                                   cAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATggg                                                      <  1:421787/93‑1 (MQ=255)

                                    aaaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                     <  1:903309/93‑1 (MQ=255)

                                        tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAcc                                                 <  1:4030872/93‑1 (MQ=255)

                                        tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAcc                                                 <  1:4305481/93‑1 (MQ=255)

                                        tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAcc                                                 <  1:5262613/93‑1 (MQ=255)

                                         gctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccg                                                >  2:1310126/1‑93 (MQ=255)

                                           tgGAAATCTTTTTCCTGCTTTTTCCACTCGGGTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgcc                                              >  2:2754726/1‑93 (MQ=255)

                                             gAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACcgccgc                                            >  1:3071923/1‑93 (MQ=255)

                                               aaTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCtt                                          >  1:2001854/1‑93 (MQ=255)

                                                 tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAGCGCCGCtttt                                        >  1:3316681/1‑93 (MQ=255)

                                                  cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                       >  1:5396236/1‑93 (MQ=255)

                                                  cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                       >  2:5127035/1‑93 (MQ=255)

                                                   tttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCa                                      <  1:1744899/93‑1 (MQ=255)

                                                       tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                  >  2:3396288/1‑93 (MQ=255)

                                                       tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                  <  2:29346/93‑1 (MQ=255)

                                                         cTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                >  2:1341082/1‑93 (MQ=255)

                                                          tGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGc                               >  1:5802587/1‑93 (MQ=255)

                                                           gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCt                              >  1:5639886/1‑93 (MQ=255)

                                                           gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGATTTTTAGCACCGCt                              >  2:504122/1‑93 (MQ=255)

                                                           gCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCtt                             >  1:3627572/1‑93 (MQ=255)

                                                             tttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCttt                            <  2:1032068/93‑1 (MQ=255)

                                                             tttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCttt                            <  2:2257120/93‑1 (MQ=255)

                                                              ttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                           <  2:674099/93‑1 (MQ=255)

                                                              ttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                           <  2:4765764/93‑1 (MQ=255)

                                                                 tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                        >  2:212531/1‑93 (MQ=255)

                                                                 tCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                        >  2:1187116/1‑93 (MQ=255)

                                                                  ccACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTCATGGGATACCACCGCTTTTCAGCACCGCTTTCgcgc                       >  2:2842641/1‑93 (MQ=255)

                                                                  ccACTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCgcgc                       <  1:2633332/93‑1 (MQ=255)

                                                                    aCTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGccc                     <  2:6254969/93‑1 (MQ=255)

                                                                     cTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                    >  1:2189444/1‑93 (MQ=255)

                                                                     cTCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                    >  1:2962712/1‑93 (MQ=255)

                                                                      tCGGTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                   >  1:4922389/1‑93 (MQ=255)

                                                                       cGGTTTGGGTTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAtt                  >  2:6135320/1‑93 (MQ=255)

                                                                         gTTTGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTc                >  1:2542765/1‑93 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               >  2:5165233/1‑93 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               >  2:4366104/1‑93 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               <  1:3233096/93‑1 (MQ=255)

                                                                          tttGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa               <  1:1007498/93‑1 (MQ=255)

                                                                            tGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt             <  2:3692734/93‑1 (MQ=255)

                                                                            tGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt             <  2:2640434/93‑1 (MQ=255)

                                                                            tGGATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt             <  2:235299/93‑1 (MQ=255)

                                                                             ggATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTa            >  2:958024/1‑93 (MQ=255)

                                                                              gATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa           <  1:197119/93‑1 (MQ=255)

                                                                              gATTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa           <  1:1350529/93‑1 (MQ=255)

                                                                               aTTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAAt          >  1:1554160/1‑93 (MQ=255)

                                                                               aTTGAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTACATTAAt          <  2:5985374/93‑1 (MQ=255)

                                                                                  gAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCt       >  1:2999916/1‑93 (MQ=255)

                                                                                  gAGGATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCt       <  2:69618/93‑1 (MQ=255)

                                                                                   aGGATTGCACC‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg      <  1:3365442/93‑1 (MQ=255)

                                                                                    ggATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt     >  1:1422330/1‑93 (MQ=255)

                                                                                     gATTGCA‑GCCCCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt     >  2:4906926/1‑93 (MQ=255)

                                                                                     gATTGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc    >  1:6232673/1‑93 (MQ=255)

                                                                                       ttGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt  >  1:2489124/1‑93 (MQ=255)

                                                                                       ttGCA‑GC‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt  <  1:2919794/93‑1 (MQ=255)

TTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCC‑CCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCT  >  NC_000913/3098959‑3099137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`