BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,585	+G	coding (953/2286 nt)	glcF ←	glycolate oxidase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,582	1	.	G	100.0%	588.1 / NA	185	R319P (CGC→CCC)	glcF	glycolate oxidase iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (103/82); total (103/82)

Only 100 of 207 total aligned reads displayed.

GTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG  >  NC_000913/3123491‑3123669

gTTCCGCGTACATGCGCCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                        <  1:4710149/93‑1 (MQ=255)

  tCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggg                                                                                      >  2:3262630/1‑93 (MQ=255)

   ccGCGTCCATGCGACGGGGTTTACACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gggg                                                                                     <  2:4345694/93‑2 (MQ=255)

   ccGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gggg                                                                                     >  2:2237189/1‑92 (MQ=255)

    cgcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATCGCGGAATAAAGCAGCCGATAGC‑ggggc                                                                                    >  2:1429882/1‑91 (MQ=255)

      cgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggggcaa                                                                                  >  2:541032/1‑89 (MQ=255)

          cATGCGACCGGGGTTAGACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGcc                                                                               >  1:1819614/1‑93 (MQ=255)

          cATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGcc                                                                               <  1:1759373/93‑1 (MQ=255)

           aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGcca                                                                              >  1:4543302/1‑93 (MQ=255)

           aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGaca                                                                              >  2:3084271/1‑93 (MQ=255)

            tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccac                                                                             <  2:2600813/93‑1 (MQ=255)

            tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccac                                                                             >  1:5166779/1‑93 (MQ=255)

             gCGGCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccacc                                                                            <  1:1971397/93‑1 (MQ=255)

               gACCGGGGTGAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGTTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAt                                                                          <  2:596067/93‑1 (MQ=255)

                aCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtc                                                                         <  2:848141/93‑1 (MQ=255)

                aCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtc                                                                         <  1:1508833/93‑1 (MQ=255)

                aCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtc                                                                         >  1:2017498/1‑93 (MQ=255)

                 ccGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtct                                                                        >  1:4808768/1‑93 (MQ=255)

                   ggggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTcc                                                                      >  2:4880354/1‑93 (MQ=255)

                    gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCg                                                                     >  1:838919/1‑93 (MQ=255)

                     ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCgg                                                                    >  2:1195289/1‑93 (MQ=255)

                       ttAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCa                                                                  >  2:2554299/1‑93 (MQ=255)

                        tAAACCCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCAc                                                                 <  1:4749477/93‑1 (MQ=255)

                        tAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCACAGCCACCATCTCCGGCAc                                                                 >  2:3851460/1‑93 (MQ=255)

                        tAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCAc                                                                 >  1:2910143/1‑93 (MQ=255)

                          aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTa                                                               <  2:6040229/93‑1 (MQ=255)

                          aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTa                                                               >  1:4943801/1‑93 (MQ=255)

                             ccgccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAg                                                            <  2:3480972/92‑1 (MQ=255)

                             acGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAg                                                            <  2:6094008/93‑1 (MQ=255)

                             acGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAg                                                            <  2:778779/93‑1 (MQ=255)

                                ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCgg                                                         >  1:984244/1‑93 (MQ=255)

                                 cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCggg                                                        <  1:2616210/93‑1 (MQ=255)

                                  gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGt                                                       >  2:2206113/1‑93 (MQ=255)

                                  gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGt                                                       <  2:5507882/93‑1 (MQ=255)

                                  gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGt                                                       <  1:4259681/93‑1 (MQ=255)

                                  gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCAACCATCTCCGGCACTAAAGCGGGt                                                       >  1:3189970/1‑93 (MQ=255)

                                  gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGAGAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGt                                                       <  2:3892825/93‑1 (MQ=255)

                                    aaGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCg                                                     >  1:3282450/1‑93 (MQ=255)

                                          cGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAc                                               <  1:706209/93‑1 (MQ=255)

                                           gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                              >  2:2311117/1‑93 (MQ=255)

                                           gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                              >  1:5284019/1‑93 (MQ=255)

                                            aGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg                                             >  2:4465121/1‑93 (MQ=255)

                                             gctgctGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGc                                            >  1:409621/1‑93 (MQ=255)

                                              ctgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGcc                                           >  1:5422847/1‑93 (MQ=255)

                                              ctgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGcc                                           >  2:6254782/1‑93 (MQ=255)

                                               tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          >  1:3565185/1‑93 (MQ=255)

                                               tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          >  2:2716182/1‑93 (MQ=255)

                                               tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          >  1:1015963/1‑93 (MQ=255)

                                                gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                         <  2:4218031/93‑1 (MQ=255)

                                                gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                         >  1:4901986/1‑93 (MQ=255)

                                                 ctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGc                                        >  1:2209080/1‑93 (MQ=255)

                                                  tgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCg                                       <  2:5138767/93‑1 (MQ=255)

                                                   gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      >  1:4590488/1‑93 (MQ=255)

                                                    tttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt                                     >  1:1231052/1‑93 (MQ=255)

                                                     ttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTg                                    <  2:5799561/93‑1 (MQ=255)

                                                       aaGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                  >  2:5247691/1‑93 (MQ=255)

                                                       aaGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                  <  2:3045657/93‑1 (MQ=255)

                                                       aaGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                  <  1:3461225/93‑1 (MQ=255)

                                                        aGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                 >  1:1640501/1‑93 (MQ=255)

                                                        aGCTGCTGGTGATAGCGGAATAAAGCAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                 >  2:4750865/1‑93 (MQ=255)

                                                         gctgctGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                <  2:2279499/93‑1 (MQ=255)

                                                         gctgctGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                <  2:258060/93‑1 (MQ=255)

                                                         gctgctGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                >  1:2661607/1‑93 (MQ=255)

                                                           tgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcg                              >  2:1086238/1‑93 (MQ=255)

                                                           tgctgGGGATAGCGGAATAAAGGAGCCGATAACGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGGTGCATGACCGCCAGCGTTGCGGgcg                              >  2:2875317/1‑93 (MQ=255)

                                                            gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga                             <  2:3905260/93‑1 (MQ=255)

                                                            gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga                             >  1:2083413/1‑93 (MQ=255)

                                                            gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga                             >  1:2462373/1‑93 (MQ=255)

                                                             ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgat                            >  1:1571159/1‑93 (MQ=255)

                                                             ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgat                            >  2:2093667/1‑93 (MQ=255)

                                                             ctgGTGATAGAGGAATAAAGGAGCCGATAGCGGGGAAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgag                            >  2:1615683/1‑92 (MQ=255)

                                                               ggTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                          <  2:2421089/93‑1 (MQ=255)

                                                                  gATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcgat                       >  2:359043/1‑93 (MQ=255)

                                                                    aaGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATgg                     <  2:2145814/92‑1 (MQ=255)

                                                                     aGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGa                    <  1:4869941/93‑1 (MQ=255)

                                                                     aGCGAAAGAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGa                    <  1:5921053/93‑1 (MQ=255)

                                                                      gCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAt                   >  2:4142336/1‑93 (MQ=255)

                                                                       cGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAtt                  <  1:3108883/93‑1 (MQ=255)

                                                                        ggAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAttt                 <  1:5203109/93‑1 (MQ=255)

                                                                         gAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTg                >  2:1731448/1‑93 (MQ=255)

                                                                         gAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTg                >  2:3302226/1‑93 (MQ=255)

                                                                         gAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTg                >  2:4616795/1‑93 (MQ=255)

                                                                          aaTAAGGGAGCCGATAGCGGGGCAAAGCCACCATCGCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa               <  2:4963254/93‑1 (MQ=255)

                                                                          aaTAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa               <  1:5080088/93‑1 (MQ=255)

                                                                           aTAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt              >  1:5781999/1‑93 (MQ=255)

                                                                            aaaaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAtt             <  2:4499290/92‑1 (MQ=255)

                                                                             aaaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTg            >  2:5705599/1‑93 (MQ=255)

                                                                              aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGt           >  1:548356/1‑93 (MQ=255)

                                                                               aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc          <  1:6184427/93‑1 (MQ=255)

                                                                               aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc          <  1:1876454/93‑1 (MQ=255)

                                                                                ggAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTcc         >  2:194923/1‑93 (MQ=255)

                                                                                  aGCCGATAGCGGGGCAAAGCCACCATCTCCGGCGCTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc       <  1:3197778/93‑1 (MQ=255)

                                                                                  aGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc       <  2:2356660/93‑1 (MQ=255)

                                                                                   gCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg      >  2:3193588/1‑93 (MQ=255)

                                                                                     cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGc    <  1:3715367/93‑1 (MQ=255)

                                                                                      gATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCt   >  2:395861/1‑93 (MQ=255)

                                                                                      gATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCt   >  1:5658722/1‑93 (MQ=255)

                                                                                       aTAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  2:3934331/1‑93 (MQ=255)

                                                                                       aTAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  1:4170751/1‑93 (MQ=255)

                                                                                       aTAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  1:3856787/1‑93 (MQ=255)

GTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG  >  NC_000913/3123491‑3123669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`