Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,181	(T)5→6	intergenic (+200/‑22)	ygiL → / → yi215	putative fimbrial‑like protein/IS2 hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,176	1	.	T	100.0%	174.6 / NA	54	intergenic (+195/‑27)	ygiL/yi215	putative fimbrial‑like protein/IS2 hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (39/15);  total (39/15)

AAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184084‑3184201                                                                                              |                          aaaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                            >  1:2033428/1‑93 (MQ=255) aaaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                            <  1:227088/93‑1 (MQ=255)  aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          >  1:1555212/1‑93 (MQ=255)  aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          <  2:1998119/93‑1 (MQ=255)   aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTACTACTAG‑tt                         <  2:6150891/93‑1 (MQ=255)   aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         >  1:4962303/1‑93 (MQ=255)   aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         <  1:1452541/93‑1 (MQ=255)    atatTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttt                        >  1:328435/1‑93 (MQ=255)    atatTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttt                        >  1:2847807/1‑93 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       <  1:2667392/93‑1 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  1:3033433/1‑93 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  1:5855209/1‑93 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      <  2:476374/93‑1 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      <  2:3004167/93‑1 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      >  2:3725149/1‑93 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     <  1:2953371/93‑2 (MQ=255)         tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   >  1:4058909/1‑90 (MQ=255)         tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   >  1:2963613/1‑90 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  1:4414369/1‑89 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  2:4503156/1‑89 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  <  1:2933791/93‑5 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  2:4458881/1‑89 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  1:1534312/1‑89 (MQ=255)            tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 >  1:5688427/1‑93 (MQ=255)            tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 <  1:2352261/93‑1 (MQ=255)             gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                <  1:2995998/93‑1 (MQ=255)             gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:3928157/1‑93 (MQ=255)             gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  2:1558688/1‑93 (MQ=255)             gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:2195067/1‑93 (MQ=255)             gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:670693/1‑93 (MQ=255)             gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  2:506494/1‑93 (MQ=255)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:4559898/1‑93 (MQ=255)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  1:1035976/1‑93 (MQ=255)               tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              <  2:2908714/93‑1 (MQ=255)               tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              <  2:2545611/93‑1 (MQ=255)               tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              <  2:896199/93‑1 (MQ=255)               tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              >  1:1588926/1‑93 (MQ=255)               tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              >  1:3651679/1‑93 (MQ=255)                cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             >  1:5144818/1‑93 (MQ=255)                cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             >  2:5568964/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  1:4865168/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  1:3425528/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  2:189238/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  1:1350797/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:5256437/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  1:3825426/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  1:4786884/1‑93 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  1:3975146/93‑1 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  1:3666366/93‑1 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:5580086/93‑1 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  2:1580824/1‑93 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  2:1445414/1‑93 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  2:794686/1‑93 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  2:280572/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:2727890/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        <  1:2870891/93‑1 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:1989058/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        <  1:657494/93‑1 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        <  2:3952261/93‑1 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:794263/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:894947/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATAACACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:3382604/1‑93 (MQ=255)                      ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       <  1:5534078/93‑1 (MQ=255)                       cGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgag      >  1:2793504/1‑93 (MQ=255)                        gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  1:5259177/1‑93 (MQ=255)                        gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  2:2540919/1‑93 (MQ=255)                         ttattgGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGCGATCGTCTTAATACTAGTTTTTTAGACTAGTCATTGgagca    >  2:2162553/1‑91 (MQ=11)                         ttattaGACTGTCACCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    <  2:538942/93‑1 (MQ=11)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  1:2362350/1‑93 (MQ=16)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  2:489427/1‑93 (MQ=16)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    <  2:4043753/93‑1 (MQ=16)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  2:5761990/1‑93 (MQ=16)                          tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  1:749710/1‑93 (MQ=12)                          tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  2:6240864/1‑93 (MQ=12)                          tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   <  2:1924724/93‑1 (MQ=12)                          tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  2:1796258/1‑93 (MQ=12)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  1:3071236/1‑93 (MQ=9)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  1:6094919/1‑93 (MQ=9)                                                                                              |                          AAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184084‑3184201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑