| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 269765 = | NA (NA) | 282 (1.150) | 133/184 | 0.1 | 98.3% | coding (300/405 nt) | yi91a | hypothetical protein |
| ? | NC_000913 | = 370983 | 5 (0.020) | coding (536/882 nt) | mhpC | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase | |||||
Only 100 of 503 total aligned reads displayed.
CATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATCACCTGGAAA > NC_000913/370891‑371075 | tGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtg < 1:3745274‑M2/93‑3 (MQ=255) taaacaacagaccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGat < 1:1916117‑M2/6‑1 (MQ=255) gtttgtttACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtag > 2:4501781‑M2/1‑88 (MQ=255) aaacaacagaccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgccagattgaatctacaATGGata > 2:4120192‑M2/87‑93 (MQ=255) aacaacagaccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggcgttgcattgacagattgaatctacaATGGatat > 1:3102975‑M2/86‑93 (MQ=255) ttgtttACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagat < 2:484834‑M2/93‑8 (MQ=255) acagaccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTc > 2:1298005‑M2/82‑93 (MQ=255) cagaccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCg < 1:2769950‑M2/13‑1 (MQ=255) tACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaat > 2:1578253‑M2/1‑81 (MQ=255) gaccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGtt < 2:3074143‑M2/15‑1 (MQ=255) gCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattgg > 2:3977269‑M2/1‑78 (MQ=255) gCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattgg > 1:5064874‑M2/1‑78 (MQ=255) ccgagaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGtttt < 2:457699‑M2/17‑1 (MQ=255) cGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtc < 2:2303309‑M2/93‑18 (MQ=255) gATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtca < 1:1271061‑M2/93‑19 (MQ=255) gaaagacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGAt > 2:3413797‑M2/73‑93 (MQ=255) aTGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGTCTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaa > 1:1447422‑M2/1‑74 (MQ=255) aTGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaa < 1:6126884‑M2/93‑20 (MQ=255) agacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATAcc > 2:1040090‑M2/70‑93 (MQ=255) gacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCa < 1:3673990‑M2/25‑1 (MQ=255) gacactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCa < 2:1759819‑M2/25‑1 (MQ=255) acactgaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAg > 1:510168‑M2/68‑93 (MQ=255) aaCCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaa < 1:4445453‑M2/93‑25 (MQ=255) aCCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaac < 2:5328521‑M2/93‑26 (MQ=255) aCCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaac > 1:5557974‑M2/1‑68 (MQ=255) ccGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaaca > 1:6039259‑M2/1‑67 (MQ=255) gAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagt < 2:5606841‑M2/93‑29 (MQ=255) gaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGAtt < 1:4320549‑M2/31‑1 (MQ=255) gaagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGAtt > 2:5751671‑M2/63‑93 (MQ=255) aagttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGAttt < 2:5096763‑M2/32‑1 (MQ=255) ttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGAc < 2:2485228‑M2/35‑1 (MQ=255) ttcaaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGAc < 2:3145220‑M2/35‑1 (MQ=255) tATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgt < 1:733082‑M2/93‑34 (MQ=255) aTTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtg > 1:4731208‑M2/1‑59 (MQ=255) tAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaa < 2:858647‑M2/93‑37 (MQ=255) aaaacaccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGa > 2:3021536‑M2/56‑93 (MQ=255) aGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaa > 2:4978038‑M2/1‑55 (MQ=255) gCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaac < 1:6282000‑M2/93‑40 (MQ=255) caccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGcc > 1:1764612‑M2/52‑93 (MQ=255) cGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaaca > 2:6153330‑M2/1‑53 (MQ=255) cGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaaca > 1:3168757‑M2/1‑53 (MQ=255) gACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTTATGAGtgtagattcaattggtcagcgcaacagttatgtgaaaacat > 1:5372001‑M2/1‑52 (MQ=255) gACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacat < 2:5893333‑M2/93‑42 (MQ=255) gACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacat > 1:4618565‑M2/1‑52 (MQ=255) gACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacat > 1:1099837‑M2/1‑52 (MQ=255) ccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCt > 2:750663‑M2/50‑93 (MQ=255) ccgaaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCt < 2:383156‑M2/44‑1 (MQ=255) tGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggg < 1:4135071‑M2/93‑45 (MQ=255) aaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGtt < 2:4249565‑M2/47‑1 (MQ=255) aaagagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGtt > 1:1219612‑M2/47‑93 (MQ=255) aaTCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggt > 2:2525066‑M2/1‑47 (MQ=255) aTCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgacaacatggggtt < 2:2208339‑M2/93‑48 (MQ=255) aTCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggtt > 2:5493431‑M2/1‑46 (MQ=255) aTCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggtt > 2:290984‑M2/1‑46 (MQ=255) gagataattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGa < 1:5861931‑M2/50‑1 (MQ=255) aGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcg < 1:5810592‑M2/93‑51 (MQ=255) cTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcgga > 2:2869509‑M2/1‑41 (MQ=255) tttATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggag > 1:3423464‑M2/1‑40 (MQ=255) aattgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAgcgc > 1:4188589‑M2/39‑93 (MQ=255) tATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggt < 1:28312‑M2/93‑56 (MQ=255) tATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggt > 1:6206520‑M2/1‑38 (MQ=255) tgaaaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGcc < 1:2331435‑M2/58‑1 (MQ=255) gTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttt > 1:845396‑M2/1‑34 (MQ=255) gTCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttt < 1:4952692‑M2/93‑60 (MQ=255) aaggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGa > 2:4652671‑M2/33‑93 (MQ=255) tCAGCCGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatgggggtgcggaggtttttt < 1:3257458‑M2/93‑61 (MQ=255) aggggtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGaa > 1:2105252‑M2/32‑93 (MQ=255) gtgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGCCCGACGCCCTGTTTGAAGCGCGCCTGaataat < 1:1006352‑M2/66‑1 (MQ=255) ccGACTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaat > 2:2955298‑M2/1‑29 (MQ=255) tgttgcattgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAataata < 2:2134915‑M2/67‑1 (MQ=255) cTATCGAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgagg < 1:2587548‑M2/93‑69 (MQ=255) attgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGt > 1:3990775‑M2/21‑93 (MQ=255) attgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGt < 1:5644586‑M2/73‑1 (MQ=255) attgacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGt > 1:5918375‑M2/21‑93 (MQ=255) gAAAACCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaac > 2:3524550‑M2/1‑20 (MQ=255) gacagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTcgc > 1:5286068‑M2/18‑93 (MQ=255) aaaaCCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaaca > 1:3882179‑M2/1‑19 (MQ=255) acagattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTcgcg > 2:2684436‑M2/17‑93 (MQ=255) aaCCTGAAGCTGATGATtgtagattcaatttgtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatt > 1:3587717‑M2/1‑17 (MQ=255) aaCCTGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatt < 1:3800387‑M2/93‑77 (MQ=255) agattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGcgcc > 2:1117540‑M2/15‑93 (MQ=255) agattgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGcgcc > 1:4835253‑M2/15‑93 (MQ=255) attgaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGcgccgc > 2:1432935‑M2/13‑93 (MQ=255) tGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttaca > 1:5456116‑M2/1‑13 (MQ=255) tGAAGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggagggtttttgaatgaggcgaacatttaca < 2:2592860‑M2/93‑81 (MQ=255) taatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGAt < 2:1954435‑M2/84‑1 (MQ=255) gaatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGAt < 2:5016895‑M2/84‑1 (MQ=255) aaGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttacagc > 2:6286456‑M2/1‑11 (MQ=255) aaGCTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttacagc > 2:4374009‑M2/1‑11 (MQ=255) aatctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATc < 2:3626419‑M2/85‑1 (MQ=255) atctacaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATCa < 1:968181‑M2/86‑1 (MQ=255) cTGATGATtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttacagcaga < 2:3275771‑M2/93‑86 (MQ=255) tgatgaTtgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttacagcagag > 2:3700357‑M2/1‑7 (MQ=255) atgattgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttacagcagagga > 2:1266747‑M2/1‑5 (MQ=255) atgattgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgaggcgaacatttacagcagagga < 2:6070109‑M2/93‑89 (MQ=255) acaATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATCACCTg < 1:2473519‑M2/90‑1 (MQ=255) aATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATCACCTGGa > 2:1708166‑M2/2‑93 (MQ=255) ttgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgagacgaacatttacagcagaggaaaaa < 2:2055389‑M2/93‑93 (MQ=255) ttgtagattcaattggtcaacgcaacagttatgtgaaaacatggggttgcggaggttttttgaatgagacgaacatttacagcagaggaaaaa < 2:475172‑M2/93‑93 (MQ=255) tgGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATCACCTGGaaa < 1:1067404/93‑1 (MQ=255) | CATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATGGATATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAATATGCTGTCGCGCCGCGATCACCTGGAAA > NC_000913/370891‑371075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |