| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,096,579 | 0 | T | C | 60.0% | 13.3 / 10.1 | 15 | intergenic (+527/+209) | ycdU/serX | hypothetical protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base C (5/4); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCG > NC_000913/1096536‑1096636 | ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGa < 1:2628199/93‑1 (MQ=0) ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACt < 1:3512996/93‑1 (MQ=12) ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACt < 1:5257119/93‑1 (MQ=255) tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtc < 2:534658/93‑1 (MQ=16) tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGATGGGGGGGATTGACtc > 1:2031840/1‑93 (MQ=255) tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtc > 2:1060798/1‑93 (MQ=255) tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtc > 2:5953516/1‑93 (MQ=255) cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcg < 1:5797494/93‑1 (MQ=16) aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgc > 1:3304444/1‑93 (MQ=255) tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGACtcgct > 2:4065382/1‑93 (MQ=255) tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgct < 2:5154619/93‑1 (MQ=255) ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgctt < 1:226022/93‑1 (MQ=255) ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgctt < 1:3334761/93‑1 (MQ=255) cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc > 1:310016/1‑93 (MQ=12) cccccGGGGTGTGCAATATACGAAAAAAAACCCCGTCCTTTCGTACGACCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttc < 1:1845208/93‑1 (MQ=11) ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttcg > 2:1345872/1‑93 (MQ=255) | TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCG > NC_000913/1096536‑1096636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |