BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	374,060	G→A	E323E (GAG→GAA)	mhpE →	4‑hydroxy‑2‑ketovalerate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	374,060	0	G	A	100.0%	819.4 / NA	220	E323E (GAG→GAA)	mhpE	4‑hydroxy‑2‑ketovalerate aldolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (117/103); total (117/103)

Only 100 of 239 total aligned reads displayed.

TCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAC  >  NC_000913/373970‑374149

tCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAggaaga                                                                                         <  2:629389/93‑1 (MQ=255)

tCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAggaaga                                                                                         >  2:6170648/1‑93 (MQ=255)

tCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAggaaga                                                                                         <  1:3221922/93‑1 (MQ=255)

  aCTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGata                                                                                       <  1:934369/93‑1 (MQ=255)

  aCTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGata                                                                                       >  1:3820751/1‑93 (MQ=255)

   cTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGatat                                                                                      <  2:3344626/93‑1 (MQ=255)

    tgtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATg                                                                                     <  2:1856434/93‑1 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGGTATTCTCGTTGAGCTGGGCAAACGCCGGATGGGTGGCGGCCAGGAAGATATGa                                                                                    >  2:4694054/1‑93 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    >  2:228581/1‑93 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGa                                                                                    >  2:144240/1‑93 (MQ=255)

     gtgAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGGTGGCGGCCAGGAAGATATGa                                                                                    >  2:4309907/1‑93 (MQ=255)

         aaCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGt                                                                                >  2:5618923/1‑93 (MQ=255)

         aaCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGt                                                                                >  2:5196059/1‑93 (MQ=255)

          aCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGtt                                                                               >  2:391053/1‑93 (MQ=255)

          aCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGtt                                                                               <  2:1081208/93‑1 (MQ=255)

          aCGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGtt                                                                               <  1:3333855/93‑1 (MQ=255)

           cggcggcggCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTg                                                                              >  1:1490747/1‑93 (MQ=255)

            ggcggcggcGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGa                                                                             <  1:5468266/93‑1 (MQ=255)

             gcggcggcgCGTTATGTCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGAc                                                                            <  2:5583919/93‑1 (MQ=255)

             gcggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGAc                                                                            >  1:5822843/1‑93 (MQ=255)

             gcggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGAc                                                                            >  2:1767763/1‑93 (MQ=255)

               ggcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGt                                                                          <  1:51264/93‑1 (MQ=255)

                gcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTg                                                                         <  1:63016/93‑1 (MQ=255)

                gcggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTg                                                                         <  1:5682730/93‑1 (MQ=255)

                  ggcgCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgc                                                                       >  2:4120867/1‑93 (MQ=255)

                   gcgcGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcg                                                                      >  2:366301/1‑93 (MQ=255)

                   gcgcGTTATGGCTTAAGTGCGGAGGATATTCTCGTTTAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGCAGATATGATCGTTCACGTGgcg                                                                      >  2:2327590/1‑93 (MQ=255)

                    cgcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcgc                                                                     <  1:2901028/93‑1 (MQ=255)

                    cgcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcgc                                                                     <  2:1325732/93‑1 (MQ=255)

                    cgcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGgcgc                                                                     >  2:255336/1‑93 (MQ=255)

                     gcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCt                                                                    >  1:1164572/1‑93 (MQ=255)

                     gcgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCt                                                                    <  1:2194975/93‑1 (MQ=255)

                      cgTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTg                                                                   <  2:4514941/93‑1 (MQ=255)

                       gTTATGGCTTAAGTGCGGTGTATATTCTCGTTGAGCTGGGCAAGCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTgg                                                                  <  1:1036049/93‑1 (MQ=255)

                       gTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTgg                                                                  <  2:3825124/93‑1 (MQ=255)

                       gTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTgg                                                                  <  1:1665158/93‑1 (MQ=255)

                        ttATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGa                                                                 <  2:4455400/93‑1 (MQ=255)

                        ttATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGa                                                                 >  2:293221/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  2:5275345/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  2:3551409/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  2:2523292/1‑93 (MQ=255)

                         tATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGAt                                                                >  2:1985532/1‑93 (MQ=255)

                           tGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCt                                                              >  2:3832026/1‑93 (MQ=255)

                             gCTTAAGTGCGGTGGTTATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTgc                                                            >  2:417723/1‑93 (MQ=255)

                             gCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTgc                                                            >  1:490103/1‑93 (MQ=255)

                             gCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTgc                                                            >  1:431602/1‑93 (MQ=255)

                             gCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTgc                                                            <  1:4652908/93‑1 (MQ=255)

                                tAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGca                                                         >  2:4903052/1‑93 (MQ=255)

                                      cGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaaca                                                   >  2:785820/1‑93 (MQ=255)

                                       ggTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaacaa                                                  <  1:4224004/93‑1 (MQ=255)

                                       ggTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGcaacaacaa                                                  >  1:3488850/1‑93 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGTAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 >  2:5105179/1‑93 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 >  1:5745912/1‑93 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 >  1:2280789/1‑93 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 >  1:6192102/1‑93 (MQ=255)

                                        gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACaaa                                                 >  2:4840839/1‑93 (MQ=255)

                                         tGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCGACAACAaat                                                >  2:5167889/1‑93 (MQ=255)

                                         tGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGGTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCTCAACAACAaat                                                >  1:6151168/1‑93 (MQ=255)

                                          tgATATTCTCGTTGAGCTGGGCATACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaata                                               <  2:6020107/92‑1 (MQ=255)

                                          ggATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaata                                               <  2:4959319/93‑1 (MQ=255)

                                            atatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAaataag                                             >  2:6184482/1‑92 (MQ=255)

                                             tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatg                                            >  2:5366100/1‑93 (MQ=255)

                                             tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatg                                            <  1:2113497/93‑1 (MQ=255)

                                             tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatg                                            <  2:1316003/93‑1 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           <  2:3968974/93‑1 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           >  1:727549/1‑93 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           >  1:4092494/1‑93 (MQ=255)

                                              atTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatga                                           <  2:5786943/93‑1 (MQ=255)

                                               ttCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAatgat                                          <  1:2350344/93‑1 (MQ=255)

                                                 ctcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAAtgatga                                        <  1:1315242/93‑1 (MQ=255)

                                                  tcGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGGTCTGCGCAACAACAAATAATGATGAc                                       <  1:5297553/93‑1 (MQ=255)

                                                     ttGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGc                                    >  1:3928243/1‑93 (MQ=255)

                                                     ttGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGc                                    >  1:2732850/1‑93 (MQ=255)

                                                       gAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCg                                  <  1:5228013/93‑1 (MQ=255)

                                                         gCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCgag                                <  1:2813034/93‑1 (MQ=255)

                                                            gggCAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAAc                             >  2:4150500/1‑93 (MQ=255)

                                                             gacAAACGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACg                            <  2:30008/91‑1 (MQ=255)

                                                                aaaCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGc                         >  2:4737174/1‑93 (MQ=255)

                                                                aaaCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGc                         >  1:4764730/1‑93 (MQ=255)

                                                                aaaCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGc                         >  1:745829/1‑93 (MQ=255)

                                                                 aaCGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCa                        >  1:1439256/1‑93 (MQ=255)

                                                                   cGCCGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCAtt                      <  1:4462737/93‑1 (MQ=255)

                                                                      cGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTg                   >  2:563477/1‑93 (MQ=255)

                                                                      cGGATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTg                   >  1:2572689/1‑93 (MQ=255)

                                                                       ggATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGt                  >  1:4666280/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 >  2:5461431/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 >  1:4971026/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 >  2:4874623/1‑93 (MQ=255)

                                                                        gATGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTa                 >  1:3861429/1‑93 (MQ=255)

                                                                         aTGGTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTAt                <  1:1767763/93‑1 (MQ=255)

                                                                           ggTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGc              >  2:3503711/1‑93 (MQ=255)

                                                                            gTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGcc             >  2:2427971/1‑93 (MQ=255)

                                                                            gTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGcc             >  1:2777516/1‑93 (MQ=255)

                                                                            gTTGGCGGCCAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGcc             >  2:1907570/1‑93 (MQ=255)

                                                                                gcggcgAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATc         <  1:5813972/93‑1 (MQ=255)

                                                                                    ccAGGAAGATGTGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAg     <  2:747338/93‑1 (MQ=255)

                                                                                     cAGGAAGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGc    >  1:1888763/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  >  2:1610422/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  >  1:5988909/1‑93 (MQ=255)

                                                                                       ggaagaTATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAc  <  1:4846768/93‑1 (MQ=255)

TCACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGTGCGGTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCAC  >  NC_000913/373970‑374149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`