BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,668	G→A	L93L (TTG→TTA)	yrhB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,668	0	G	A	98.6%	786.3 / NA	218	L93L (TTG→TTA)	yrhB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (154/61); minor base C (0/3); total (154/64)
Fisher's exact test for biased strand distribution p-value = 2.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCG  >  NC_000913/3582578‑3582757

aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAcc                                                                                         >  2:887581/1‑93 (MQ=255)

 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAccc                                                                                        <  2:2967834/93‑1 (MQ=255)

 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAccc                                                                                        >  2:5252172/1‑93 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCt                                                                                       >  1:4725318/1‑93 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCt                                                                                       <  2:1760865/93‑1 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCt                                                                                       >  1:4980863/1‑93 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGAGTACGAAATAAAAAAGGCTACCTTCGGCTTACCCt                                                                                       >  2:5387038/1‑93 (MQ=255)

   cAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTg                                                                                      >  1:2345992/1‑93 (MQ=255)

    aGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCCGa                                                                                     >  2:379799/1‑93 (MQ=255)

     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                    >  2:1125080/1‑93 (MQ=255)

     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                    <  2:707002/93‑1 (MQ=255)

     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                    >  1:5084078/1‑93 (MQ=255)

      tggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACa                                                                                   >  2:5976635/1‑93 (MQ=255)

          gAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAAt                                                                               <  1:2102619/93‑1 (MQ=255)

           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                              >  2:832124/1‑93 (MQ=255)

             attcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGc                                                                            >  2:573710/1‑93 (MQ=255)

             attcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGc                                                                            <  2:2004436/93‑1 (MQ=255)

               tcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGccc                                                                          <  1:5506487/93‑1 (MQ=255)

                 attcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctc                                                                        <  2:1735454/93‑1 (MQ=255)

                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                       >  2:2471773/1‑93 (MQ=255)

                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                       >  2:5630368/1‑93 (MQ=255)

                   tctcTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGACCTCtt                                                                      >  2:3731089/1‑93 (MQ=255)

                    ctctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTc                                                                     <  1:2647397/93‑1 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGACCTCTTcc                                                                    >  2:5064932/1‑93 (MQ=255)

                       tGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCa                                                                  >  1:1248756/1‑93 (MQ=255)

                         ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACg                                                                >  2:2782386/1‑93 (MQ=255)

                         ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACg                                                                >  2:16954/1‑93 (MQ=255)

                         ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACg                                                                >  1:5386338/1‑93 (MQ=255)

                         ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACg                                                                >  2:4507079/1‑93 (MQ=255)

                          gAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGa                                                               >  2:755442/1‑93 (MQ=255)

                          gAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGa                                                               >  2:4798707/1‑93 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTATCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                           <  1:4443584/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                           <  1:3246750/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                           <  2:1610680/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                           >  2:3398677/1‑93 (MQ=255)

                               gCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAgg                                                          >  1:1466920/1‑93 (MQ=255)

                                cAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAggg                                                         <  1:3736149/93‑1 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        <  1:5470642/93‑1 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        >  1:2904117/1‑93 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        >  1:421841/1‑93 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                        >  1:4137095/1‑93 (MQ=255)

                                  aaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGcc                                                       <  2:3943801/93‑1 (MQ=255)

                                  aaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGcc                                                       >  2:2524967/1‑93 (MQ=255)

                                  aaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGcc                                                       >  1:4580721/1‑93 (MQ=255)

                                  aaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGcc                                                       >  2:1187917/1‑93 (MQ=255)

                                   aaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCg                                                      >  2:5688180/1‑93 (MQ=255)

                                    aCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGc                                                     >  2:3130460/1‑93 (MQ=255)

                                    aCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGc                                                     >  2:5345292/1‑93 (MQ=255)

                                       gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTaa                                                  >  1:5669459/1‑93 (MQ=255)

                                       gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTaa                                                  >  2:2618998/1‑93 (MQ=255)

                                         tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGACGCTAAcc                                                >  2:140842/1‑93 (MQ=255)

                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                               >  2:1644397/1‑93 (MQ=255)

                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                               <  1:3372633/93‑1 (MQ=255)

                                            aagaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTa                                             <  1:6261204/93‑1 (MQ=255)

                                             agaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                            >  2:4880472/1‑93 (MQ=255)

                                             agaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                            <  1:1640344/93‑1 (MQ=255)

                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAga                                           >  2:925146/1‑92 (MQ=255)

                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGt                                           <  2:3385682/93‑1 (MQ=255)

                                                atatCTACAGGATTACGAAATAAAAAAGGCTGCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAc                                         <  1:2839577/93‑1 (MQ=255)

                                                atatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAc                                         <  2:4068964/93‑1 (MQ=255)

                                                  atCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAccc                                       >  2:2733555/1‑93 (MQ=255)

                                                  atCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAccc                                       <  1:1227709/93‑1 (MQ=255)

                                                   tCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcccc                                      >  2:4346417/1‑93 (MQ=255)

                                                     tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc                                    >  2:2666711/1‑93 (MQ=255)

                                                     tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc                                    >  1:813430/1‑93 (MQ=255)

                                                       cAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgccg                                  >  2:5045768/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                >  2:1513886/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                >  1:1326866/1‑93 (MQ=255)

                                                          gATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTa                               >  1:3937026/1‑93 (MQ=255)

                                                           aTTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTaa                              >  1:1075388/1‑93 (MQ=255)

                                                              aCGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTACatc                           >  2:4421303/1‑93 (MQ=255)

                                                               cGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatca                          >  1:3577947/1‑93 (MQ=255)

                                                                gAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcat                         >  1:5857860/1‑93 (MQ=255)

                                                                 aaaTAAAAAAGGCTCCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcatc                        <  1:5708040/93‑1 (MQ=255)

                                                                  aaaaaaaaaGGCTCCCTTCGGCTTCCCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                       <  2:4717919/90‑1 (MQ=255)

                                                                  aaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                       >  2:2367833/1‑93 (MQ=255)

                                                                  aaTAAAAAAGCCTCCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATcc                       <  2:1899223/93‑1 (MQ=255)

                                                                     aaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAcc                    >  2:67704/1‑93 (MQ=255)

                                                                      aaaaaGGCTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCg                   <  1:1797724/93‑1 (MQ=255)

                                                                        aaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAg                 >  2:3056583/1‑93 (MQ=255)

                                                                        aaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAg                 >  1:34138/1‑93 (MQ=255)

                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg               >  2:3496794/1‑93 (MQ=255)

                                                                            gCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGcgcg             >  1:4926909/1‑93 (MQ=255)

                                                                             cTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCgg            >  2:1235532/1‑93 (MQ=255)

                                                                              tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg           >  2:3986700/1‑93 (MQ=255)

                                                                                ctttCGTCTTACCCTGAAAAAATAGCCCTCTCCCCACGAAGAGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTc         <  1:3648207/91‑1 (MQ=255)

                                                                                ccTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTc         >  2:2120662/1‑93 (MQ=255)

                                                                                ccTTCGGCTTACCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTACCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTc         <  1:2165604/93‑1 (MQ=255)

                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg        >  2:326985/1‑93 (MQ=255)

                                                                                  ttCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg       >  2:5559024/1‑93 (MQ=255)

                                                                                  ttCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg       >  1:3807638/1‑93 (MQ=255)

                                                                                   tCGGCTTCCCCTGACAAAAGAGCCCTCTCCCCACGAAGGGGGCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa      <  1:3494132/93‑1 (MQ=255)

                                                                                   tCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa      >  2:1454609/1‑93 (MQ=255)

                                                                                    cGGCTTCCCCTAAAAAAATCCCCCTCTCCCCACGAAGAGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGAt     <  2:4381633/93‑1 (MQ=255)

                                                                                    cGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGAt     >  1:4207362/1‑93 (MQ=255)

                                                                                     gtcTTCCCCTGACAAAATAGCCCTCTCCCCACGAAGAGGCCCGCTACCCTTAGTTCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATg    <  2:3681249/91‑1 (MQ=255)

                                                                                     ggCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATg    >  2:6148758/1‑93 (MQ=255)

                                                                                      gCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc   >  2:100280/1‑93 (MQ=255)

                                                                                       cTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTGAATCATCCACCGAGCGCGGGTCGGATgcg  <  1:4656198/93‑1 (MQ=255)

                                                                                       cTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgcg  >  2:5947089/1‑93 (MQ=255)

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCG  >  NC_000913/3582578‑3582757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`