BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	404,199	C→T	A224T (GCA→ACA)	proC ←	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	404,199	0	C	T	100.0%	819.6 / NA	220	A224T (GCA→ACA)	proC	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (121/99); total (121/99)

Only 100 of 238 total aligned reads displayed.

CGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCC  >  NC_000913/404109‑404288

cGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGtcc                                                                                         >  2:5590887/1‑93 (MQ=255)

 gATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGtccc                                                                                        >  2:3877028/1‑93 (MQ=255)

 gATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGtccc                                                                                        >  2:5896406/1‑93 (MQ=255)

 gATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGtccc                                                                                        >  1:2621596/1‑93 (MQ=255)

  aTCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGtcccc                                                                                       <  1:3041213/93‑1 (MQ=255)

   tCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCg                                                                                      >  2:2553765/1‑93 (MQ=255)

    cACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCgg                                                                                     >  1:4468839/1‑93 (MQ=255)

    cACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCgg                                                                                     >  1:3528754/1‑93 (MQ=255)

     aCTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGa                                                                                    <  1:4598221/93‑1 (MQ=255)

     aCTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGa                                                                                    >  1:2784201/1‑93 (MQ=255)

      cTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGAt                                                                                   >  1:1077380/1‑93 (MQ=255)

      cTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGAt                                                                                   >  1:4723570/1‑93 (MQ=255)

        gcagcaCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGt                                                                                 <  2:483878/93‑1 (MQ=255)

        gcagcaCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGt                                                                                 >  1:2921111/1‑93 (MQ=255)

           gcaCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTtct                                                                              <  1:3244246/93‑1 (MQ=255)

             aCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTcc                                                                            >  2:1363526/1‑93 (MQ=255)

             aCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTcc                                                                            >  1:5098422/1‑93 (MQ=255)

             aCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTcc                                                                            <  1:5029581/93‑1 (MQ=255)

             aCGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTcc                                                                            >  2:4947077/1‑93 (MQ=255)

              cGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTccc                                                                           <  2:2495412/93‑1 (MQ=255)

              cGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTccc                                                                           <  1:5500527/93‑1 (MQ=255)

               ggAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCg                                                                          >  2:2220853/1‑93 (MQ=255)

                   gCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTc                                                                      <  1:6196971/93‑1 (MQ=255)

                    ccTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTcc                                                                     >  1:6083980/1‑93 (MQ=255)

                     cTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCa                                                                    <  2:1521594/93‑1 (MQ=255)

                     cTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCa                                                                    >  1:4302450/1‑93 (MQ=255)

                      tttCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAg                                                                   >  1:1033385/1‑93 (MQ=255)

                        tctcTGCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCa                                                                 <  1:4119697/93‑1 (MQ=255)

                         ctctTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCAc                                                                <  2:1248341/93‑1 (MQ=255)

                           ctTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCa                                                              <  1:2446540/93‑1 (MQ=255)

                           ctTCCAGTACGCGTACCGCTGCAAGGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCa                                                              <  2:5968192/93‑1 (MQ=255)

                            ttCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCAt                                                             >  2:5394479/1‑93 (MQ=255)

                            ttCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCAt                                                             <  1:2902198/93‑1 (MQ=255)

                             tCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCAtt                                                            >  2:3362542/1‑93 (MQ=255)

                             tCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCAtt                                                            <  1:3573984/93‑1 (MQ=255)

                             tCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGGTTCCAGCACCact                                                            >  2:4462233/1‑91 (MQ=255)

                             tCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGACGTTCTCCCGTTTCCAGCACCAtt                                                            >  2:5694708/1‑93 (MQ=255)

                              ccAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCAttt                                                           >  1:3565921/1‑93 (MQ=255)

                              ccAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCAttt                                                           <  2:3009178/93‑1 (MQ=255)

                                  tACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGc                                                       >  1:5970877/1‑93 (MQ=255)

                                    cgcgTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCgg                                                     <  1:5384456/93‑1 (MQ=255)

                                     gcgTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGa                                                    >  1:914021/1‑93 (MQ=255)

                                       gTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAAc                                                  >  1:1960539/1‑93 (MQ=255)

                                        tACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAAcc                                                 >  1:70884/1‑93 (MQ=255)

                                          ccGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCa                                               >  2:1750926/1‑93 (MQ=255)

                                          ccGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCa                                               >  1:1943748/1‑93 (MQ=255)

                                            gCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCAtt                                             >  2:4643339/1‑93 (MQ=255)

                                               tCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTAcc                                          <  2:398803/93‑1 (MQ=255)

                                               tCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTAcc                                          <  2:1339603/93‑1 (MQ=255)

                                                 aaTGGTGTTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGc                                        <  2:4104504/93‑1 (MQ=255)

                                                  aTGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGcc                                       >  2:3569067/1‑93 (MQ=255)

                                                  aTGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGcc                                       >  1:2302239/1‑93 (MQ=255)

                                                  aTGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGcc                                       <  1:5184809/93‑1 (MQ=255)

                                                   tggtggTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCt                                      <  1:5737268/93‑1 (MQ=255)

                                                    ggtggtGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTg                                     >  2:6206479/1‑93 (MQ=255)

                                                    ggtggtGCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTg                                     <  1:1577878/93‑1 (MQ=255)

                                                     gtggtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGa                                    >  2:1243967/1‑93 (MQ=255)

                                                     gtggtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCACCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGa                                    <  1:3382084/93‑1 (MQ=255)

                                                      tggtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAg                                   <  2:1499404/93‑1 (MQ=255)

                                                      tggtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAg                                   <  2:5416296/93‑1 (MQ=255)

                                                            cTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaa                             >  2:4283662/1‑93 (MQ=255)

                                                            cTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaa                             >  1:372058/1‑93 (MQ=255)

                                                            cTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaa                             <  2:3657111/93‑1 (MQ=255)

                                                            cTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaa                             >  1:4925897/1‑93 (MQ=255)

                                                            cTCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaa                             >  1:2413930/1‑93 (MQ=255)

                                                             tCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaaa                            >  2:3040658/1‑93 (MQ=255)

                                                             tCCCGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCaaa                            >  2:2158708/1‑93 (MQ=255)

                                                               ccGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAAtt                          >  2:2595617/1‑93 (MQ=255)

                                                                cGGTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAAttt                         >  1:3804374/1‑93 (MQ=255)

                                                                 ggTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTta                        >  1:1202197/1‑93 (MQ=255)

                                                                 ggTGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTta                        <  2:6125334/93‑1 (MQ=255)

                                                                   tGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTtata                      <  1:5466614/93‑1 (MQ=255)

                                                                   tGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTtata                      <  1:163304/93‑1 (MQ=255)

                                                                   tGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTtata                      <  1:1750926/93‑1 (MQ=255)

                                                                   tGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTtata                      <  1:4031486/93‑1 (MQ=255)

                                                                   tGAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTtata                      <  1:3996825/93‑1 (MQ=255)

                                                                    gAGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATAc                     <  2:2730295/93‑1 (MQ=255)

                                                                     aGCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACg                    <  2:1821509/93‑1 (MQ=255)

                                                                      gCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGc                   <  2:6066051/93‑1 (MQ=255)

                                                                      gCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGc                   <  1:4986673/93‑1 (MQ=255)

                                                                      gCAGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGc                   >  1:5891598/1‑93 (MQ=255)

                                                                        aGACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCt                 <  1:1388325/93‑1 (MQ=255)

                                                                         gACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTg                >  2:637183/1‑93 (MQ=255)

                                                                         gACCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTg                <  2:4692337/93‑1 (MQ=255)

                                                                          gccATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCAGCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTgg               <  1:479696/92‑1 (MQ=255)

                                                                          aCCATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCACATTTATAGGCCTgg               >  1:1771159/1‑93 (MQ=255)

                                                                            cATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgc             <  2:883961/93‑1 (MQ=255)

                                                                            cATATCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgc             <  2:3239890/93‑1 (MQ=255)

                                                                              tatCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgcgc           >  2:1163705/1‑93 (MQ=255)

                                                                              tatCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgcgc           >  1:3410551/1‑93 (MQ=255)

                                                                              tatCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgcgc           >  1:2143152/1‑93 (MQ=255)

                                                                                tCTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGt         >  2:4749514/1‑93 (MQ=255)

                                                                                 cTTTCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTg        <  1:2662231/93‑1 (MQ=255)

                                                                                   ttCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGc      >  2:5765542/1‑93 (MQ=255)

                                                                                    tCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCa     >  2:2077893/1‑93 (MQ=255)

                                                                                    tCAGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCa     >  1:649233/1‑93 (MQ=255)

                                                                                      aGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATc   >  2:3485104/1‑93 (MQ=255)

                                                                                      aGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATc   >  2:1689046/1‑93 (MQ=255)

                                                                                      aGTGTCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATc   <  1:312685/93‑1 (MQ=255)

                                                                                       gtgtCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATcc  <  2:415216/93‑1 (MQ=255)

CGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCC  >  NC_000913/404109‑404288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`