BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,614,182	T→C	F124L (TTC→CTC)	nikC →	NikC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,614,182	0	T	C	100.0%	845.3 / NA	259	F124L (TTC→CTC)	nikC	NikC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (126/133); total (126/133)

Only 100 of 266 total aligned reads displayed.

GGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTG  >  NC_000913/3614092‑3614271

ggcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCAACATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTc                                                                                         >  1:5298599/1‑93 (MQ=255)

 gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGctct                                                                                        <  2:1604571/93‑1 (MQ=255)

  cAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCtt                                                                                       <  2:4716063/93‑1 (MQ=255)

   aGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCttt                                                                                      >  1:5833788/1‑93 (MQ=255)

    gcgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCTCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTa                                                                                     <  1:2152049/93‑1 (MQ=255)

    gcgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTa                                                                                     <  1:4208376/93‑1 (MQ=255)

    gcgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTa                                                                                     >  1:5527902/1‑93 (MQ=255)

     cgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTAt                                                                                    <  1:3484619/93‑1 (MQ=255)

         gcgTTGATTGGCGGGCGCGTTGATCAGGCCACCAGGCGCGTCGCCGATATGTTTATGACCTGCCCGACCTCGATTCTGTCGCTCTTTATGGtt                                                                                <  2:4948851/91‑1 (MQ=255)

          ggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTg                                                                               <  1:608887/93‑1 (MQ=255)

           gTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTgg                                                                              >  2:4048735/1‑93 (MQ=255)

           gTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTgg                                                                              >  2:5386174/1‑93 (MQ=255)

            ttGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGc                                                                             >  1:5640057/1‑93 (MQ=255)

            ttGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGc                                                                             <  1:1064888/93‑1 (MQ=255)

              taTTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGt                                                                           <  2:840357/92‑1 (MQ=255)

              gATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGt                                                                           >  1:3844024/1‑93 (MQ=255)

                 tGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCt                                                                        <  1:3776600/93‑1 (MQ=255)

                   gcgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCg                                                                      >  1:5765173/1‑93 (MQ=255)

                      ggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCa                                                                   >  1:4512311/1‑93 (MQ=255)

                      ggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCa                                                                   >  2:1955677/1‑93 (MQ=255)

                      ggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCa                                                                   >  1:193845/1‑93 (MQ=255)

                        cgcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCAcc                                                                 <  1:263688/93‑1 (MQ=255)

                        cgcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCAcc                                                                 <  2:2338906/93‑1 (MQ=255)

                         gcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCg                                                                >  1:4992820/1‑93 (MQ=255)

                         gcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCg                                                                <  2:3013033/93‑1 (MQ=255)

                          cgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCgg                                                               <  2:599568/93‑1 (MQ=255)

                           gTTGATCAGGCCCCCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCggg                                                              <  2:95938/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  2:1161052/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  2:1385105/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  2:5154266/93‑1 (MQ=255)

                            ttGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGc                                                             <  2:6290172/93‑1 (MQ=255)

                               aTCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGa                                                          <  1:4027280/93‑1 (MQ=255)

                                tCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGAc                                                         >  2:1054468/1‑93 (MQ=255)

                                   ggCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGGTGACCaa                                                      >  2:4362033/1‑93 (MQ=255)

                                   ggCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCaa                                                      <  1:2744688/93‑1 (MQ=255)

                                    gCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAAc                                                     <  1:223784/93‑1 (MQ=255)

                                    gCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAAc                                                     >  2:1472586/1‑93 (MQ=255)

                                      caccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                   >  1:4629321/1‑93 (MQ=255)

                                       accaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTa                                                  >  2:4783276/1‑93 (MQ=255)

                                       accaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTa                                                  >  1:4432791/1‑93 (MQ=255)

                                        ccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTaa                                                 <  1:4265587/93‑1 (MQ=255)

                                        ccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTaa                                                 >  1:712627/1‑93 (MQ=255)

                                         caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTatt                                                <  1:3558577/93‑3 (MQ=255)

                                         caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAAt                                                <  2:3428127/93‑1 (MQ=255)

                                         caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAAt                                                <  1:372698/93‑1 (MQ=255)

                                         caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAAt                                                <  2:718483/93‑1 (MQ=255)

                                           tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTa                                              <  2:4109988/93‑1 (MQ=255)

                                            gcgcGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTat                                             <  1:2386795/93‑1 (MQ=255)

                                            gcgcGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTat                                             <  2:2667962/93‑1 (MQ=255)

                                              gcgTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcg                                           <  1:1757884/93‑1 (MQ=255)

                                               cgTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgc                                          >  2:3320869/1‑93 (MQ=255)

                                               cgTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgc                                          <  1:5718588/93‑1 (MQ=255)

                                                gTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                         >  2:2028319/1‑93 (MQ=255)

                                                gTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                         <  1:4055872/93‑1 (MQ=255)

                                                 tCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTTGCGTGCTCGGCACCGGGCTGACCGACGTGATTatcgcca                                        >  2:5707139/1‑93 (MQ=255)

                                                  cGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccat                                       <  1:2560165/93‑1 (MQ=255)

                                                   gctgATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      >  1:485735/4‑93 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      <  2:902481/93‑1 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                      <  2:2990969/93‑1 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   >  2:2253369/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   <  2:3218279/93‑1 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   >  2:2871883/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   <  2:331259/93‑1 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   >  2:1748920/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                   >  1:6238372/1‑93 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGccc                                  >  1:5862670/1‑93 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGccc                                  <  2:4566516/93‑1 (MQ=255)

                                                         atGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTg                                <  2:4381152/93‑1 (MQ=255)

                                                          tGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGt                               >  2:220040/1‑93 (MQ=255)

                                                          tGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGt                               <  1:5057863/93‑1 (MQ=255)

                                                           gTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGACATCGCCCTGTc                              >  2:3705567/1‑93 (MQ=255)

                                                               aTGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCAc                          <  1:309186/93‑1 (MQ=255)

                                                                tGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACt                         <  1:902381/93‑1 (MQ=255)

                                                                 gACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTg                        <  2:2902897/93‑1 (MQ=255)

                                                                  aCCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGCCCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                       >  1:592094/1‑93 (MQ=255)

                                                                  aCCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                       >  2:3623303/1‑93 (MQ=255)

                                                                  aCCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                       <  1:6119958/93‑1 (MQ=255)

                                                                   ccTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTggg                      <  2:3184454/93‑1 (MQ=255)

                                                                    cTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGc                     <  2:355910/93‑1 (MQ=255)

                                                                     ttCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCg                    <  1:5363547/93‑1 (MQ=255)

                                                                      tCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGt                   <  2:221698/93‑1 (MQ=255)

                                                                      tCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGt                   >  1:3368629/1‑93 (MQ=255)

                                                                       cccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                  >  1:1354745/1‑93 (MQ=255)

                                                                          gACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTa               >  2:3473545/1‑93 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt              >  1:5168338/1‑93 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt              >  2:616862/1‑93 (MQ=255)

                                                                            ccTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATg             >  1:1566076/1‑93 (MQ=255)

                                                                              tCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCa           <  1:2335504/93‑1 (MQ=255)

                                                                              tCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCa           >  1:3912111/1‑93 (MQ=255)

                                                                               cGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc          <  1:795184/93‑1 (MQ=255)

                                                                                gATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCATCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACg         >  1:4790605/1‑93 (MQ=255)

                                                                                gATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACg         >  1:1913110/1‑93 (MQ=255)

                                                                                gATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACg         >  2:5443246/1‑93 (MQ=255)

                                                                                 aTTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGc        >  2:5501535/1‑93 (MQ=255)

                                                                                 aTTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGc        >  1:1543864/1‑93 (MQ=255)

                                                                                  ttCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCa       >  1:321341/1‑93 (MQ=255)

                                                                                   tCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGcct      >  2:2514521/1‑91 (MQ=255)

                                                                                    cTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATg     <  2:3909540/93‑1 (MQ=255)

                                                                                      gTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGt   >  2:4003176/1‑93 (MQ=255)

                                                                                      gTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGt   <  2:3121104/93‑1 (MQ=255)

GGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTG  >  NC_000913/3614092‑3614271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`