BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,624,135	A→G	N100N (AAT→AAC)	b3485 ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,624,135	0	A	G	99.6%	876.7 / NA	267	N100N (AAT→AAC)	b3485	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (138/128); minor base C (0/1); total (138/129)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

AAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATA  >  NC_000913/3624045‑3624224

 aaTGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGttt                                                                                        <  2:3281472/93‑1 (MQ=255)

 aaTGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGttt                                                                                        >  1:5639637/1‑93 (MQ=255)

 aaTGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGttt                                                                                        <  2:3507574/93‑1 (MQ=255)

 aaTGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGttt                                                                                        >  2:6040059/1‑93 (MQ=255)

  aTGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTa                                                                                       >  1:4457727/1‑93 (MQ=255)

   tGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTAt                                                                                      <  1:375530/93‑1 (MQ=255)

   tGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTAt                                                                                      <  2:3713489/93‑1 (MQ=255)

   tGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTAt                                                                                      <  1:1795906/93‑1 (MQ=255)

    gCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATc                                                                                     <  1:502931/93‑1 (MQ=255)

     ccTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcg                                                                                    <  2:2972570/93‑1 (MQ=255)

     ccTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcg                                                                                    >  1:4162862/1‑93 (MQ=255)

      cTGGCTGATGCGCATGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgc                                                                                   <  2:3690132/93‑1 (MQ=255)

      cTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgc                                                                                   <  2:932821/93‑1 (MQ=255)

      cTGGCTCATGCGCGTGGCATCGACGTGCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATcgc                                                                                   <  2:613011/93‑1 (MQ=255)

         gCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGaa                                                                                <  2:2055566/93‑1 (MQ=255)

           tCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAgg                                                                              <  1:2751225/93‑1 (MQ=255)

            cATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGt                                                                             <  2:4944585/93‑1 (MQ=255)

            cATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGt                                                                             <  1:3293014/93‑1 (MQ=255)

            cATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGt                                                                             <  1:3223255/93‑1 (MQ=255)

             aTGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGta                                                                            <  2:1124554/93‑1 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           <  2:1533459/93‑1 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           >  1:4768584/1‑93 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           >  1:2180010/1‑93 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           >  1:2358299/1‑93 (MQ=255)

              tGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtat                                                                           >  1:36380/1‑93 (MQ=255)

               gcgcGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtata                                                                          <  2:3228459/93‑1 (MQ=255)

               gcgcGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtata                                                                          <  1:46235/93‑1 (MQ=255)

               gcgcGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGtata                                                                          <  1:2475280/93‑1 (MQ=255)

                cgcgTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAg                                                                         <  1:5627316/93‑1 (MQ=255)

                 gcgTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGc                                                                        <  1:5572073/93‑1 (MQ=255)

                 gcgTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGc                                                                        <  1:5089637/93‑1 (MQ=255)

                   gTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGt                                                                      >  1:1918114/1‑93 (MQ=255)

                   gTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGt                                                                      <  1:4375177/93‑1 (MQ=255)

                    tGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTc                                                                     >  1:976569/1‑93 (MQ=255)

                       cATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCgg                                                                  >  1:5344914/1‑93 (MQ=255)

                       cATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCgg                                                                  <  1:151469/93‑1 (MQ=255)

                        aTCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGc                                                                 <  2:505834/93‑1 (MQ=255)

                         tCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCg                                                                >  1:3414753/1‑93 (MQ=255)

                         tCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCg                                                                >  1:1645841/1‑93 (MQ=255)

                         tCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCg                                                                >  1:2628792/1‑93 (MQ=255)

                           gACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTc                                                              >  2:2432494/1‑93 (MQ=255)

                              gTTCACCTGAATATCCGGCTGGCGTTCTGCGAGGACATCAAGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCCGCGTCCAg                                                           >  2:5434321/1‑93 (MQ=255)

                               ttCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGt                                                          >  1:4393436/1‑93 (MQ=255)

                               ttCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGt                                                          >  1:5782992/1‑93 (MQ=255)

                                tCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTc                                                         <  1:3007403/93‑1 (MQ=255)

                                tCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTc                                                         <  2:2311252/93‑1 (MQ=255)

                                  aCCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCg                                                       >  2:4696234/1‑93 (MQ=255)

                                   ccTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCgg                                                      >  1:1347772/1‑93 (MQ=255)

                                    cTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTGCgga                                                     >  1:665780/1‑92 (MQ=255)

                                    cTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGc                                                     >  2:3565177/1‑93 (MQ=255)

                                     tGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGca                                                    >  1:2211494/1‑93 (MQ=255)

                                     tGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGca                                                    <  1:777532/93‑1 (MQ=255)

                                      gAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcat                                                   <  1:281803/93‑1 (MQ=255)

                                      gAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcat                                                   <  2:4342050/93‑1 (MQ=255)

                                         tatCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatcca                                                <  2:3951723/93‑1 (MQ=255)

                                         tatCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGcatcca                                                >  2:2529523/1‑93 (MQ=255)

                                            ccGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCAtct                                             <  1:4922168/93‑1 (MQ=255)

                                             cGGCTGGCGTCCGGCGAGGACATCACGCTGAAACTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGGCCGGCCTTCAtctc                                            >  2:203761/1‑93 (MQ=255)

                                               gCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCAt                                          >  2:2166636/1‑93 (MQ=255)

                                               gCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCAt                                          >  1:3489164/1‑93 (MQ=255)

                                               gCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATACATCTCAt                                          >  1:5522717/1‑93 (MQ=255)

                                                cTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATc                                         <  2:5295236/93‑1 (MQ=255)

                                                  ggCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcgg                                       >  2:3838340/1‑93 (MQ=255)

                                                  ggCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcgg                                       >  1:4294598/1‑93 (MQ=255)

                                                   gCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcggc                                      <  2:3352218/93‑1 (MQ=255)

                                                    cGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcggcg                                     <  1:4710833/93‑1 (MQ=255)

                                                     gTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATcggcgg                                    >  2:1752713/1‑93 (MQ=255)

                                                      tCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGt                                   >  2:4263828/1‑93 (MQ=255)

                                                      tCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGt                                   <  2:5753708/93‑1 (MQ=255)

                                                           cGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGctca                              >  2:5269033/1‑93 (MQ=255)

                                                           cGAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatca                              >  1:3061035/1‑93 (MQ=255)

                                                            gAGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatcat                             >  2:798291/1‑93 (MQ=255)

                                                             aGGACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGatcatc                            <  1:1155155/93‑1 (MQ=255)

                                                              ggACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCAtct                           >  1:720447/1‑93 (MQ=255)

                                                              ggACATCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATAAtct                           >  1:1093411/1‑93 (MQ=255)

                                                                  aTCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCgg                       >  2:3019012/1‑93 (MQ=255)

                                                                  aTCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCgg                       >  2:2615480/1‑93 (MQ=255)

                                                                   tCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGt                      <  1:4034689/93‑1 (MQ=255)

                                                                   tCACGCTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGt                      <  1:5883137/93‑1 (MQ=255)

                                                                        cTGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCaa                 >  1:6175576/1‑93 (MQ=255)

                                                                         tGAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCaaa                >  2:5832930/1‑93 (MQ=255)

                                                                          gAAAATTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCaaaa               <  1:4063780/93‑1 (MQ=255)

                                                                           aaaaTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCaaaaa              <  1:5014687/93‑1 (MQ=255)

                                                                              aTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCa           >  2:5471915/1‑93 (MQ=255)

                                                                              aTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCa           >  1:2940551/1‑93 (MQ=255)

                                                                              aTTAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCa           >  2:627652/1‑93 (MQ=255)

                                                                                tAGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACg         >  1:6217638/1‑93 (MQ=255)

                                                                                 aGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGGGGCAAAAAACACgg        >  1:6076125/1‑93 (MQ=255)

                                                                                 aGGCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCAAAAACCACgg        >  2:5166869/1‑93 (MQ=255)

                                                                                  ggCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACggc       >  1:2095993/1‑92 (MQ=255)

                                                                                  ggCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGa       <  2:1354787/93‑1 (MQ=255)

                                                                                   gCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGAc      <  2:2788015/93‑1 (MQ=255)

                                                                                   gCGGAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGAc      <  2:5698728/93‑1 (MQ=255)

                                                                                     ggAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGa    >  1:2198494/1‑93 (MQ=255)

                                                                                     ggAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGa    >  1:3980773/1‑93 (MQ=255)

                                                                                     ggAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGa    <  1:1283732/93‑1 (MQ=255)

                                                                                      gAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGAt   >  2:4460600/1‑93 (MQ=255)

                                                                                      gAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGAt   <  1:831823/93‑1 (MQ=255)

                                                                                      gAATGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGAt   >  1:1020778/1‑93 (MQ=255)

                                                                                       aaTGTTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATa  <  1:2042657/93‑1 (MQ=255)

AAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATA  >  NC_000913/3624045‑3624224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`