BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,228	A→G	R271R (CGT→CGC)	yhiN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,228	0	A	G	99.6%	726.1 / NA	227	R271R (CGT→CGC)	yhiN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (110/116); minor base T (1/0); total (111/116)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTG  >  NC_000913/3634138‑3634317

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                        <  2:35340/93‑1 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                        <  1:591567/93‑1 (MQ=255)

  tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgct                                                                                       >  1:236085/1‑93 (MQ=255)

  tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgct                                                                                       >  2:513442/1‑93 (MQ=255)

  tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgct                                                                                       >  1:5442274/1‑93 (MQ=255)

     gCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctgct                                                                                    <  2:1031879/93‑1 (MQ=255)

       ccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCa                                                                                  <  1:3874973/93‑1 (MQ=255)

         gAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAtt                                                                                >  2:4287847/1‑93 (MQ=255)

           gTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCa                                                                              >  1:3121333/1‑93 (MQ=255)

           gTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCa                                                                              >  1:3746917/1‑93 (MQ=255)

              gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                           >  1:3849533/1‑93 (MQ=255)

              gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                           >  1:2637614/1‑93 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                       >  2:5319880/1‑93 (MQ=255)

                    aGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGttt                                                                     <  2:4957523/93‑1 (MQ=255)

                    aGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGttt                                                                     <  2:5690302/93‑1 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                    >  2:908726/1‑93 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAGCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  2:1171844/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  1:4900021/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  2:1282737/93‑1 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  >  2:3845889/1‑93 (MQ=255)

                        ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                                 <  1:4133277/93‑1 (MQ=255)

                        ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                                 <  2:1837301/93‑1 (MQ=255)

                          caaccaacCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGt                                                               >  2:3810224/1‑93 (MQ=255)

                           aaccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTc                                                              <  1:2748502/93‑1 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGGTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  1:1253934/1‑93 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  2:2971045/1‑93 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  1:6222/1‑93 (MQ=255)

                             ccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCca                                                            <  2:5423421/93‑1 (MQ=255)

                              caaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcac                                                           <  1:4177943/93‑1 (MQ=255)

                               aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                          >  2:3346403/1‑93 (MQ=255)

                               aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                          >  2:3199950/1‑93 (MQ=255)

                                accGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAt                                                         <  2:3627239/93‑1 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        <  2:695416/93‑1 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        >  1:3061317/1‑93 (MQ=255)

                                  cGCTTCGGTAGATGAACCGTCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATcc                                                       >  1:608329/1‑93 (MQ=255)

                                  cGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATcc                                                       >  2:2739236/1‑93 (MQ=255)

                                   gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCCGGAAGGTTTCTAGGTCCACATCCg                                                      >  1:1800184/1‑93 (MQ=255)

                                   gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                      >  2:2121410/1‑93 (MQ=255)

                                   gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                      >  1:688713/1‑93 (MQ=255)

                                   gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                      <  1:83925/93‑1 (MQ=255)

                                     ttCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    >  2:4249587/1‑93 (MQ=255)

                                     ttCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    >  1:2520572/1‑93 (MQ=255)

                                     tgcGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    <  2:2244831/91‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   <  1:1865603/93‑1 (MQ=255)

                                        ggTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGc                                                 >  2:1923863/1‑93 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                                >  1:4537505/1‑93 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                                <  1:1546355/93‑1 (MQ=255)

                                          tAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCAGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAg                                               >  2:4425301/1‑93 (MQ=255)

                                           aGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGa                                              >  2:559886/1‑93 (MQ=255)

                                             aTGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAtt                                            <  1:769744/93‑1 (MQ=255)

                                              tGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTg                                           >  2:4109441/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          <  1:3361442/93‑1 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          >  2:1439829/1‑93 (MQ=255)

                                                 aCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATg                                        <  1:206178/93‑1 (MQ=255)

                                                    gCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTg                                     <  1:3320197/93‑1 (MQ=255)

                                                     ccAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGa                                    >  1:2795976/1‑93 (MQ=255)

                                                      cAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAc                                   >  1:4777864/1‑93 (MQ=255)

                                                       aGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACa                                  >  2:4543333/1‑93 (MQ=255)

                                                       aGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACa                                  >  2:2570022/1‑93 (MQ=255)

                                                        gtgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGCTTGATGCTGGCaa                                 >  2:829269/1‑93 (MQ=255)

                                                        gtgtggTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaa                                 >  2:4104185/1‑93 (MQ=255)

                                                         tgtgtTTTTCAGGATTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGCTTGATGCTGACaat                                >  1:5783434/1‑92 (MQ=255)

                                                           tgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAtt                              <  2:4580219/93‑1 (MQ=255)

                                                            gtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTc                             <  2:6021452/93‑1 (MQ=255)

                                                              ttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccc                           <  2:3488376/93‑1 (MQ=255)

                                                                ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                         <  2:3688310/93‑1 (MQ=255)

                                                                ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTCTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                         <  1:4091139/93‑1 (MQ=255)

                                                                ttCAGGCTTTGATTCAGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                         <  2:1989953/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      <  2:5181796/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      <  1:789084/93‑1 (MQ=255)

                                                                     gCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTg                    >  2:855691/1‑93 (MQ=255)

                                                                     gCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGGTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTg                    >  2:3578691/1‑93 (MQ=255)

                                                                      cTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGc                   >  1:5549869/1‑93 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                  <  1:254899/93‑1 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                  >  1:1333668/1‑93 (MQ=255)

                                                                        ttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCa                 <  2:3116014/93‑1 (MQ=255)

                                                                          gATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGt               >  1:3630839/1‑93 (MQ=255)

                                                                          gATTCGGATGTGCGTTGCGCTGCTCACTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGt               <  2:5092478/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  1:2111024/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  1:4575643/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  1:2002551/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  1:3599355/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCAAATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  2:5951182/93‑1 (MQ=255)

                                                                            ttCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAg             <  2:1601496/93‑1 (MQ=255)

                                                                              cGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCt           <  2:2339497/93‑1 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          <  2:240302/93‑1 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          >  1:5110887/1‑93 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          <  2:1128972/93‑1 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          <  1:1620200/93‑1 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg         >  1:432243/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg         >  1:1090580/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg         >  2:593853/1‑93 (MQ=255)

                                                                                gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg         >  1:3184120/1‑93 (MQ=255)

                                                                                 aTGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGa        <  1:1478984/93‑1 (MQ=255)

                                                                                 aTGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGa        >  2:2179286/1‑93 (MQ=255)

                                                                                  tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa       >  2:1678027/1‑93 (MQ=255)

                                                                                   gtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaaa      <  2:5212581/93‑1 (MQ=255)

                                                                                       gTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTg  >  1:2217611/1‑93 (MQ=255)

                                                                                       gTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTg  >  2:6249598/1‑93 (MQ=255)

                                                                                       gTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTg  <  1:3952120/93‑1 (MQ=255)

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTG  >  NC_000913/3634138‑3634317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`