BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,944	T→C	H68H (CAT→CAC)	uspA →	universal stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,944	0	T	C	100.0%	922.3 / NA	282	H68H (CAT→CAC)	uspA	universal stress protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (156/126); total (156/126)

Only 100 of 290 total aligned reads displayed.

CGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGC  >  NC_000913/3637854‑3638033

cGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGc                                                                                         <  1:2833775/93‑1 (MQ=255)

 gTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCa                                                                                        <  1:6253723/93‑1 (MQ=255)

 gTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCa                                                                                        >  1:1780533/1‑93 (MQ=255)

  tAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCAc                                                                                       >  1:4335666/1‑93 (MQ=255)

    gATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTg                                                                                     <  1:397933/93‑1 (MQ=255)

      tGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGAc                                                                                   <  1:830/93‑1 (MQ=255)

      tGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGAc                                                                                   <  2:6295280/93‑1 (MQ=255)

        tAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCg                                                                                 >  1:6100296/1‑93 (MQ=255)

          aaCTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAg                                                                               >  2:2462819/1‑93 (MQ=255)

             tactCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCtt                                                                            >  2:3150509/1‑93 (MQ=255)

             tactCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCtt                                                                            >  2:2824476/1‑93 (MQ=255)

             tactCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCtt                                                                            <  2:5252457/93‑1 (MQ=255)

             tactCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCtt                                                                            >  2:4876601/1‑93 (MQ=255)

                tctGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTcc                                                                         <  1:917365/93‑1 (MQ=255)

                tctGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTcc                                                                         >  2:434253/1‑93 (MQ=255)

                tctGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTcc                                                                         >  1:1689974/1‑93 (MQ=255)

                 ctGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCa                                                                        <  1:521855/93‑1 (MQ=255)

                  tGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCAc                                                                       <  1:3906503/93‑1 (MQ=255)

                   gACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACt                                                                      >  2:1581399/1‑93 (MQ=255)

                    aCCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTa                                                                     >  1:4446304/1‑93 (MQ=255)

                     ccTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTaa                                                                    <  1:4822300/93‑1 (MQ=255)

                      cTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAAt                                                                   <  2:1723927/93‑1 (MQ=255)

                       tataCACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATg                                                                  >  2:1942099/1‑93 (MQ=255)

                       tataCACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATg                                                                  >  1:5999037/1‑93 (MQ=255)

                       tataCACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATg                                                                  >  1:2054038/1‑93 (MQ=255)

                        ataCACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGc                                                                 >  1:3698552/1‑93 (MQ=255)

                         taCACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCa                                                                >  2:1824559/1‑93 (MQ=255)

                            acCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGc                                                             >  1:5318606/1‑93 (MQ=255)

                            acCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGc                                                             <  1:5048199/93‑1 (MQ=255)

                               gggCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTAc                                                          <  2:1532411/93‑1 (MQ=255)

                               gggCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTAc                                                          >  2:3523181/1‑93 (MQ=255)

                                ggCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTAcc                                                         >  1:5615368/1‑93 (MQ=255)

                                ggCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTAcc                                                         >  2:747135/1‑93 (MQ=255)

                                 gCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTAccc                                                        >  1:3557376/1‑93 (MQ=255)

                                 gCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTAccc                                                        >  1:280859/1‑93 (MQ=255)

                                  cTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCa                                                       <  2:891736/93‑1 (MQ=255)

                                    tATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAAt                                                     <  1:3787937/93‑1 (MQ=255)

                                    tATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAAt                                                     >  2:3289952/1‑93 (MQ=255)

                                    tATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAAt                                                     <  1:590248/93‑1 (MQ=255)

                                     aTTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATc                                                    >  1:704488/1‑93 (MQ=255)

                                     aTTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATc                                                    >  1:2904656/1‑93 (MQ=255)

                                      ttGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCa                                                   >  2:6083071/1‑93 (MQ=255)

                                      ttGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCa                                                   >  2:3841329/1‑93 (MQ=255)

                                        gATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACt                                                 >  1:4109120/1‑93 (MQ=255)

                                         aTGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTg                                                >  1:4418532/1‑93 (MQ=255)

                                           gtgAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGaa                                              >  2:2921978/1‑93 (MQ=255)

                                            tgAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGaaa                                             <  1:4762994/93‑1 (MQ=255)

                                             gAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAAc                                            <  1:1563525/93‑1 (MQ=255)

                                             gAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAAc                                            >  2:2597738/1‑93 (MQ=255)

                                             gAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAAc                                            >  1:195254/1‑93 (MQ=255)

                                             gAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAAc                                            <  1:3518849/93‑1 (MQ=255)

                                               aTCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAAccc                                          <  2:195482/93‑1 (MQ=255)

                                                tCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCt                                         <  1:2493239/93‑1 (MQ=255)

                                                  tGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGa                                       <  2:5683400/93‑1 (MQ=255)

                                                    ggTGATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagc                                     <  2:2304728/93‑1 (MQ=255)

                                                       gATATGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggc                                  >  2:4680569/1‑93 (MQ=255)

                                                         tatGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcag                                >  2:2641237/1‑93 (MQ=255)

                                                         tatGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcag                                >  1:2293741/1‑93 (MQ=255)

                                                          atGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcagc                               <  1:2637733/93‑1 (MQ=255)

                                                          atGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcagc                               <  1:3051082/93‑1 (MQ=255)

                                                           tGCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcagcg                              <  2:5861339/93‑1 (MQ=255)

                                                            gCAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcagcgg                             >  1:4565047/1‑93 (MQ=255)

                                                             cAGAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGagcggcagcggc                            <  1:5404912/93‑1 (MQ=255)

                                                               gAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTATCCAATCACTGAAACCCCGAGCGGCAGCGGCGa                          <  2:125597/93‑1 (MQ=255)

                                                               gAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGa                          >  2:2748922/1‑93 (MQ=255)

                                                               gAAACGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGa                          >  1:4728475/1‑93 (MQ=255)

                                                                aaaCGCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGAc                         <  1:1898818/93‑1 (MQ=255)

                                                                    gCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTgg                     <  1:3335511/93‑1 (MQ=255)

                                                                    gCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTgg                     >  2:1372307/1‑93 (MQ=255)

                                                                    gCATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTgg                     >  2:3829237/1‑93 (MQ=255)

                                                                     cATCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTggg                    <  1:1254992/93‑1 (MQ=255)

                                                                      aTCTCTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGc                   >  2:4852858/1‑93 (MQ=255)

                                                                       tctcTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAACCCCTGAGCGGCAGCGGCGACCTGGGcc                  <  1:5301641/93‑1 (MQ=255)

                                                                       tctcTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGcc                  <  2:4962341/93‑1 (MQ=255)

                                                                       tctcTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGcc                  <  2:3234455/93‑1 (MQ=255)

                                                                       tctcTGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGcc                  <  1:1014975/93‑1 (MQ=255)

                                                                         tctGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAg                >  2:4206107/1‑93 (MQ=255)

                                                                         tctGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAg                >  1:1115949/1‑93 (MQ=255)

                                                                         tctGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAg                >  2:145066/1‑93 (MQ=255)

                                                                          ctGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCTgg               >  1:4162157/1‑93 (MQ=255)

                                                                          ctGAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAgg               >  1:1370093/1‑93 (MQ=255)

                                                                            gAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGtt             >  1:5472309/1‑93 (MQ=255)

                                                                            gAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGtt             >  2:5759279/1‑93 (MQ=255)

                                                                            gAAGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGtt             <  2:4704844/93‑1 (MQ=255)

                                                                             aaGAGACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTc            >  2:3109357/1‑93 (MQ=255)

                                                                              agagACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCCGGGTCt           >  2:5158951/1‑93 (MQ=255)

                                                                              agagACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCt           <  2:5553256/93‑1 (MQ=255)

                                                                              agagACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCt           >  1:5796650/1‑93 (MQ=255)

                                                                              agagACACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGCGCCAGGTTCt           >  1:5732501/1‑93 (MQ=255)

                                                                               gagaCACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTg          <  2:348982/93‑1 (MQ=255)

                                                                               gagaCACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTg          >  2:533269/1‑93 (MQ=255)

                                                                                agaCACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTgg         <  1:3623776/93‑1 (MQ=255)

                                                                                 gaCACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGt        >  2:4187921/1‑93 (MQ=255)

                                                                                 gaCACATCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGt        >  2:2379360/1‑93 (MQ=255)

                                                                                   cacaTCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCg      <  1:4220036/93‑1 (MQ=255)

                                                                                    acaTCACGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGa     <  2:2250754/93‑1 (MQ=255)

                                                                                     catcacGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGAt    >  1:2339879/1‑93 (MQ=255)

                                                                                      atcacGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATg   >  2:2040312/1‑93 (MQ=255)

                                                                                       tcacGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGc  >  2:4578049/1‑93 (MQ=255)

                                                                                       tcacGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGc  >  1:2740646/1‑93 (MQ=255)

CGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCTGAAGAGACACATCATGCACTGACCGAGCTTTCCACTAATGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGC  >  NC_000913/3637854‑3638033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`