BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,683,091	A→G	V42A (GTC→GCC)	yhjK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,091	0	A	G	99.6%	837.3 / NA	259	V42A (GTC→GCC)	yhjK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (122/136); minor base C (0/1); total (122/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CTGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGACAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAA  >  NC_000913/3683001‑3683180

 tGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCag                                                                                        >  1:4397494/1‑93 (MQ=255)

  taCCGCGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       <  1:3802947/92‑1 (MQ=255)

  gACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       <  2:750891/93‑1 (MQ=255)

  gACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       <  2:3659265/93‑1 (MQ=255)

   aCCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCagag                                                                                      <  1:155190/93‑1 (MQ=255)

    ccACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagc                                                                                     >  2:6232203/1‑93 (MQ=255)

    ccACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagc                                                                                     <  2:1796486/93‑1 (MQ=255)

      aCGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcgg                                                                                   >  2:5512576/1‑92 (MQ=255)

      aCGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcga                                                                                   <  2:3235002/93‑1 (MQ=255)

      aCGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcga                                                                                   >  1:4480747/1‑93 (MQ=255)

       cGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcgag                                                                                  <  1:462054/93‑1 (MQ=255)

           tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              <  2:522700/93‑1 (MQ=255)

           tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              <  2:1336704/93‑1 (MQ=255)

             ccGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGc                                                                            <  2:1996915/93‑1 (MQ=255)

              cgctttAATGCTGGCAAGAATAGCTCCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCt                                                                           <  1:5721937/90‑1 (MQ=255)

               ggTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCtt                                                                          <  2:254632/93‑1 (MQ=255)

               ggTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCtt                                                                          >  1:1501157/1‑93 (MQ=255)

               ggTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCtt                                                                          <  2:870585/93‑1 (MQ=255)

                gTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCttt                                                                         >  1:4956465/1‑93 (MQ=255)

                 tttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTc                                                                        <  2:219186/93‑1 (MQ=255)

                 tttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTc                                                                        <  1:1134856/93‑1 (MQ=255)

                  ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTcc                                                                       <  2:1157793/93‑1 (MQ=255)

                  ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTcc                                                                       <  1:845625/93‑1 (MQ=255)

                  ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTcc                                                                       <  1:206654/93‑1 (MQ=255)

                   tAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGGTAAGGGTTGGCGGGCAGAGCGTGCACTGCTTTccg                                                                      >  1:4198308/1‑92 (MQ=255)

                   tAATGCTGGCAAGAATAGCTTCCACTTCGGGAATATCGCCTTCCAAAATAGCTGAAGATAAGGGTTCGCGGCCAGAGCGAGCAATGCTTTcga                                                                      <  2:5010223/93‑3 (MQ=255)

                    aaTGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAg                                                                     >  2:1241549/1‑93 (MQ=255)

                      tGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGtt                                                                   >  1:5459311/1‑93 (MQ=255)

                        cTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGc                                                                 <  1:3917438/93‑1 (MQ=255)

                         tGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCg                                                                <  1:2551099/93‑1 (MQ=255)

                          ggCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGt                                                               >  1:5276226/1‑93 (MQ=255)

                             aaGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGc                                                            >  2:5884797/1‑93 (MQ=255)

                              aGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGcc                                                           <  2:1053560/93‑1 (MQ=255)

                                aaTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgt                                                         >  2:5462113/1‑93 (MQ=255)

                                aaTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgt                                                         >  2:3971962/1‑93 (MQ=255)

                                aaTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgg                                                         >  1:538879/1‑92 (MQ=255)

                                 aTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgtg                                                        >  2:5802211/1‑93 (MQ=255)

                                  tAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgtgt                                                       >  2:3333734/1‑93 (MQ=255)

                                    gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     <  1:5172382/93‑1 (MQ=255)

                                    gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     >  2:645387/1‑93 (MQ=255)

                                      ttCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTtata                                                   <  1:2745875/93‑1 (MQ=255)

                                        ccGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGc                                                 <  1:4121317/93‑1 (MQ=255)

                                          gCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGa                                               >  2:4854771/1‑93 (MQ=255)

                                          gCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGa                                               >  2:1664640/1‑93 (MQ=255)

                                          gCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGa                                               >  2:401286/1‑93 (MQ=255)

                                            ttCGGGAATATCGCCTTTCAAAATAGCTGAAGTTAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGAtt                                             <  1:3749229/93‑1 (MQ=255)

                                              cGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTct                                           >  2:4545970/1‑93 (MQ=255)

                                               gggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctg                                          <  2:1321861/93‑1 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         <  2:2425369/93‑1 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         <  2:1296233/93‑1 (MQ=255)

                                                ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         <  2:3767738/93‑1 (MQ=255)

                                                 gAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgct                                        >  1:6078872/1‑93 (MQ=255)

                                                 gAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgct                                        <  1:4238298/93‑1 (MQ=255)

                                                  aaTATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgctg                                       <  2:2294462/93‑1 (MQ=255)

                                                  aaTATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgctg                                       <  2:1491330/93‑1 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     >  1:3679805/1‑93 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     >  2:5082456/1‑93 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     >  1:5828545/1‑93 (MQ=255)

                                                    tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGa                                     <  2:3519728/93‑1 (MQ=255)

                                                     atCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAc                                    >  1:1018652/1‑93 (MQ=255)

                                                      tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   <  1:2970114/93‑1 (MQ=255)

                                                      tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   >  2:4680241/1‑93 (MQ=255)

                                                      tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   <  1:3220493/93‑1 (MQ=255)

                                                      tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   >  1:5250059/1‑93 (MQ=255)

                                                      tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   >  1:5741583/1‑93 (MQ=255)

                                                       cGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCa                                  >  2:2366754/1‑93 (MQ=255)

                                                         ccTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGa                                <  1:6277575/93‑1 (MQ=255)

                                                          cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGTGATTCTGCTGGACCAGAt                               >  1:5584353/1‑93 (MQ=255)

                                                          cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               <  2:1892277/93‑1 (MQ=255)

                                                          cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               >  2:4095214/1‑93 (MQ=255)

                                                          cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               <  1:6124917/93‑1 (MQ=255)

                                                           tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              <  1:2630673/93‑1 (MQ=255)

                                                           tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              >  1:2176296/1‑93 (MQ=255)

                                                            ttCAAAATAGCTGAAGATAAGGGTTCGCTGGTAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATgg                             <  2:2084475/93‑1 (MQ=255)

                                                             tCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGa                            <  2:563040/93‑1 (MQ=255)

                                                              cAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGaa                           <  2:4884336/93‑1 (MQ=255)

                                                               aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          >  2:2674469/1‑93 (MQ=255)

                                                               aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          <  1:4056107/93‑1 (MQ=255)

                                                               aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          <  2:4484405/93‑1 (MQ=255)

                                                                 aaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAg                        >  2:4285791/1‑93 (MQ=255)

                                                                  aTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGc                       <  1:4318757/93‑1 (MQ=255)

                                                                  aTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGc                       <  1:1934469/93‑1 (MQ=255)

                                                                      cTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaaaa                   <  1:1327643/93‑1 (MQ=255)

                                                                       tGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAAc                  <  2:5357049/93‑1 (MQ=255)

                                                                       tGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAAc                  <  1:1602775/93‑1 (MQ=255)

                                                                       tGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAAc                  <  1:3204487/93‑1 (MQ=255)

                                                                       tGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAAc                  >  1:33961/1‑93 (MQ=255)

                                                                         aaGATAGGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACgg                <  1:2330646/93‑1 (MQ=255)

                                                                         aaGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACgg                <  2:2741664/93‑1 (MQ=255)

                                                                          aGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGt               >  1:1172343/1‑93 (MQ=255)

                                                                          aGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGt               >  2:3028980/1‑93 (MQ=255)

                                                                           gATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACTAGATGGAACAGCAAAACGGTg              >  1:3435160/1‑93 (MQ=255)

                                                                             taggggTGCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCa            <  2:4400526/90‑1 (MQ=255)

                                                                                   ttCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAAt      <  2:2886725/93‑1 (MQ=255)

                                                                                   ttCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAAt      >  1:5300080/1‑93 (MQ=255)

                                                                                    tCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATa     >  1:4780639/1‑93 (MQ=255)

                                                                                    tCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATa     <  2:4324430/93‑1 (MQ=255)

                                                                                    tCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATa     <  2:3822577/93‑1 (MQ=255)

                                                                                       cgGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATaaaa  >  2:6026871/1‑93 (MQ=255)

                                                                                       cgGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATaaaa  <  2:4597035/93‑1 (MQ=255)

CTGACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGACAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAA  >  NC_000913/3683001‑3683180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`