BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	416,945	A→G	T194A (ACG→GCG)	phoB →	positive response regulator for pho regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	416,945	0	A	G	100.0%	716.9 / NA	223	T194A (ACG→GCG)	phoB	positive response regulator for pho regulon
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/115); total (108/115)

Only 100 of 237 total aligned reads displayed.

CACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATAT  >  NC_000913/416855‑417034

atcTTTTTTGTGACGCATCTTGAGCGCGGGTACAGCGGCGAGCAGCTGTTAGACCCCGTCTGGGGAACTATCGTGTATGTGGAAGACcgcgcg                                                                                         <  2:800369/91‑1 (MQ=255)

  cTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGt                                                                                       <  1:5534760/93‑1 (MQ=255)

     ttttATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGa                                                                                    <  1:3412264/93‑1 (MQ=255)

      tttATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGAt                                                                                   <  2:3721391/93‑1 (MQ=255)

        tATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGt                                                                                 <  2:2959576/93‑1 (MQ=255)

          tGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTcc                                                                               <  2:1062413/93‑1 (MQ=255)

          tGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTcc                                                                               >  1:897198/1‑93 (MQ=255)

           gACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCca                                                                              >  1:6108801/1‑93 (MQ=255)

              gCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACAt                                                                           >  1:3335433/1‑93 (MQ=255)

              gCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACAt                                                                           <  2:4933519/93‑1 (MQ=255)

               cATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACAtt                                                                          <  2:2455247/93‑1 (MQ=255)

               cATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACAtt                                                                          <  2:5528065/93‑1 (MQ=255)

               cATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACAtt                                                                          <  1:566293/93‑1 (MQ=255)

                 tCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcg                                                                        >  1:338491/1‑93 (MQ=255)

                 tCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcg                                                                        <  1:4333427/93‑1 (MQ=255)

                 tCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcg                                                                        >  2:4743876/1‑93 (MQ=255)

                  ccTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcgt                                                                       >  1:4038926/1‑93 (MQ=255)

                  ccTGAGCGCGTGTACAGCCGAGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcgt                                                                       <  2:2261468/93‑1 (MQ=255)

                    tGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcgtcg                                                                     >  1:5332603/1‑93 (MQ=255)

                    tGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcgtcg                                                                     >  1:4852970/1‑93 (MQ=255)

                    tGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATtcgtcg                                                                     >  1:1705627/1‑93 (MQ=255)

                     gAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGc                                                                    >  2:1896684/1‑93 (MQ=255)

                     gAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGc                                                                    <  1:4836289/93‑1 (MQ=255)

                      aGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGcc                                                                   >  1:1627892/1‑93 (MQ=255)

                      aGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGcc                                                                   <  2:1759693/93‑1 (MQ=255)

                         gcgTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGc                                                                <  1:421392/93‑1 (MQ=255)

                         gcgTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGc                                                                <  1:5043529/93‑1 (MQ=255)

                            tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTa                                                             >  1:6262175/1‑93 (MQ=255)

                            tgtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTa                                                             <  1:4967893/93‑1 (MQ=255)

                             gtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTaa                                                            <  2:1162860/93‑1 (MQ=255)

                             gtACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTaa                                                            >  1:196927/1‑93 (MQ=255)

                              tACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTaaa                                                           <  2:3404027/93‑1 (MQ=255)

                              tACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTaaa                                                           <  2:5649442/93‑1 (MQ=255)

                               aCAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAg                                                          >  2:5733353/1‑93 (MQ=255)

                                cAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGc                                                         >  2:5426353/1‑93 (MQ=255)

                                cAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGc                                                         <  1:5864031/93‑1 (MQ=255)

                                 aGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCa                                                        >  1:5118282/1‑93 (MQ=255)

                                  gCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCAc                                                       >  2:3722821/1‑93 (MQ=255)

                                  gCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCAc                                                       >  2:3572618/1‑93 (MQ=255)

                                     gcgAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTgg                                                    >  2:235652/1‑93 (MQ=255)

                                      cgAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGa                                                   >  2:4149136/1‑93 (MQ=255)

                                      cgAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGa                                                   <  2:645808/93‑1 (MQ=255)

                                       gAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAg                                                  >  1:3776926/1‑93 (MQ=255)

                                         gcagcTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGcc                                                >  2:5252810/1‑93 (MQ=255)

                                         gcagcTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGcc                                                >  1:1538272/1‑93 (MQ=255)

                                         gcagcTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGcc                                                <  1:6004866/93‑1 (MQ=255)

                                         gcagcTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGcc                                                <  2:5793429/93‑1 (MQ=255)

                                         gcagcTGTTAAACCACGTCTGGGGAACTAACGCGTATGTGGAAGACCGCGCGGGCGATGTCCACATTCGTCGCCTGTGTAAAGCACTGGAGcc                                                >  1:2952739/1‑93 (MQ=255)

                                          cagcTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGccc                                               <  2:5480057/93‑1 (MQ=255)

                                           agcTGTTAACCCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcg                                              <  1:628815/93‑1 (MQ=255)

                                           agcTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcg                                              <  2:2043737/93‑1 (MQ=255)

                                             cTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcggc                                            >  1:2270216/1‑93 (MQ=255)

                                              tGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcggcg                                           >  2:3353264/1‑93 (MQ=255)

                                               gTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcggcgg                                          <  1:5461217/93‑1 (MQ=255)

                                                 tAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGc                                        <  1:3058291/93‑1 (MQ=255)

                                                  aaaCCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCa                                       <  1:3841976/93‑1 (MQ=255)

                                                  aaaCCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCa                                       <  1:6044158/93‑1 (MQ=255)

                                                   aaCCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCAt                                      <  2:2511526/93‑1 (MQ=255)

                                                     ccACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGa                                    <  1:5832051/93‑1 (MQ=255)

                                                      cACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGAc                                   <  1:5608288/93‑1 (MQ=255)

                                                      cACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGAc                                   >  1:4068394/1‑93 (MQ=255)

                                                       aCGTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGAcc                                  >  1:1447369/1‑93 (MQ=255)

                                                         gTCTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGc                                >  2:5265949/1‑93 (MQ=255)

                                                           cTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCAt                              <  1:5186147/93‑1 (MQ=255)

                                                           cTGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCAt                              >  1:369141/1‑93 (MQ=255)

                                                            tGGGGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATg                             >  1:1899236/1‑93 (MQ=255)

                                                            tGGAGAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATg                             <  1:1110066/93‑1 (MQ=255)

                                                              gggAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATggg                           >  1:5456742/1‑92 (MQ=255)

                                                              gggAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATggg                           >  1:2991634/1‑92 (MQ=255)

                                                               ggAACTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTg                          >  1:135815/1‑93 (MQ=255)

                                                                   cTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGa                      <  1:443893/93‑1 (MQ=255)

                                                                   cTAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGa                      >  2:5936495/1‑93 (MQ=255)

                                                                    tAACGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGAc                     <  1:2549142/93‑1 (MQ=255)

                                                                      aCGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCg                   >  2:3756121/1‑93 (MQ=255)

                                                                       cGTGTATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGt                  <  1:3534292/93‑1 (MQ=255)

                                                                        gtgtATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTg                 <  1:5392241/93‑1 (MQ=255)

                                                                        gtgtATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTg                 <  1:2762831/93‑1 (MQ=255)

                                                                         tgtATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgc                <  1:781619/93‑1 (MQ=255)

                                                                         tgtATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgc                >  1:2341753/1‑93 (MQ=255)

                                                                         tgtATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTgc                <  1:2182726/93‑1 (MQ=255)

                                                                         tgtATGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATCGTGCAGACCGTgc                <  2:2647335/93‑1 (MQ=255)

                                                                            aTGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGcgcg             >  2:829874/1‑93 (MQ=255)

                                                                            aTGTGGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGcgcg             >  2:3113667/1‑93 (MQ=255)

                                                                             tgtgGAGGACCGCGCTGTCGATGGCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCgg            <  1:1028110/93‑1 (MQ=255)

                                                                             tgtgGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCgg            <  2:1371483/93‑1 (MQ=255)

                                                                              gtgGAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGt           <  1:1791071/93‑1 (MQ=255)

                                                                                ggAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTAc         <  2:2880176/93‑1 (MQ=255)

                                                                                ggAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTAc         <  2:1833125/93‑1 (MQ=255)

                                                                                 gAAGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACa        >  2:3217588/1‑93 (MQ=255)

                                                                                  aaGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAg       <  2:1685582/93‑1 (MQ=255)

                                                                                  aaGACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAg       >  1:4718354/1‑93 (MQ=255)

                                                                                    gACCGGGCGCTCGATGTCCACATTCGTCGCCTGCATAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGa     <  1:1005016/93‑1 (MQ=255)

                                                                                    gACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGa     <  1:686435/93‑1 (MQ=255)

                                                                                    gACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGa     <  1:3205649/93‑1 (MQ=255)

                                                                                    gACCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGa     >  2:1962865/1‑93 (MQ=255)

                                                                                     aCCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGat    <  2:6061224/93‑1 (MQ=255)

                                                                                     aCCGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGat    >  2:4225647/1‑93 (MQ=255)

                                                                                      ccGCGCGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGata   <  1:647208/93‑1 (MQ=255)

                                                                                       cgcgcgGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGatat  >  1:4084568/1‑93 (MQ=255)

                                                                                       cgcgcgGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGatat  <  2:4531491/93‑1 (MQ=255)

CACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCATGGTGCAGACCGTGCGCGGTACAGGATAT  >  NC_000913/416855‑417034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`