BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,754,310	C→A	R382M (AGG→ATG)	yiaY ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,754,310	0	C	A	100.0%	932.3 / NA	247	R382M (AGG→ATG)	yiaY	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (136/111); total (136/111)

Only 100 of 252 total aligned reads displayed.

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCC  >  NC_000913/3754220‑3754396

gACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATc                                                                                      <  2:1330851/93‑1 (MQ=255)

 gcAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                                                                     <  2:3698061/92‑1 (MQ=255)

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                    >  1:4286131/1‑93 (MQ=255)

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                    <  2:5576118/93‑1 (MQ=255)

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                    >  2:2534654/1‑93 (MQ=255)

   aGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCt                                                                                   >  1:5245745/1‑93 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                  >  1:5276620/1‑93 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                  <  2:5791614/93‑1 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                 >  2:3647824/1‑93 (MQ=255)

     cTATCAAGAATTGACGGGAAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                 <  1:4494733/93‑1 (MQ=255)

      tATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                                <  2:5740515‑M1/93‑2 (MQ=255)

        tCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcg                                                                              >  1:593817‑M1/1‑90 (MQ=255)

            gAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataa                                                                          >  1:5001612‑M1/1‑86 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                         <  2:4406943‑M1/93‑9 (MQ=255)

             aaTTTACGGGCAAGGATTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                         <  2:2754894‑M1/93‑9 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                       <  2:1904270‑M1/93‑11 (MQ=255)

               tgtacGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                       <  2:483887‑M1/91‑11 (MQ=255)

                 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                                     <  1:4134615‑M1/93‑13 (MQ=255)

                  aCGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgcc                                                                    >  1:2207091‑M1/1‑80 (MQ=255)

                    gggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccac                                                                  >  1:3537388‑M1/1‑78 (MQ=255)

                    gggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccac                                                                  <  2:722743‑M1/93‑16 (MQ=255)

                     ggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccaca                                                                 >  1:5018532‑M1/1‑77 (MQ=255)

                       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                                               <  1:4728463‑M1/93‑19 (MQ=255)

                       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                                               >  2:5198708‑M1/1‑75 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                              >  1:3135818‑M1/1‑74 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                              >  1:1672757‑M1/1‑74 (MQ=255)

                         aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaattt                                                             >  1:1483312‑M1/1‑73 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                            <  1:3149700‑M1/93‑22 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                          >  2:3193782‑M1/1‑70 (MQ=255)

                             ttACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttc                                                         <  1:2404872‑M1/93‑25 (MQ=255)

                              tACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcg                                                        >  2:3734018‑M1/1‑68 (MQ=255)

                              tACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcg                                                        >  2:4155634‑M1/1‑68 (MQ=255)

                                cAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtg                                                      >  2:5087215‑M1/1‑66 (MQ=255)

                                cAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtg                                                      <  1:663239‑M1/93‑28 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                     >  1:5040505‑M1/1‑65 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                    >  1:3984525‑M1/1‑64 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                    >  1:2640125‑M1/1‑64 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                  >  1:2771456‑M1/1‑62 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                >  2:1689432‑M1/1‑60 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                >  2:4714860‑M1/1‑60 (MQ=255)

                                       aGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcc                                               <  1:2855432‑M1/93‑35 (MQ=255)

                                        gTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcct                                              >  1:3450148‑M1/1‑58 (MQ=255)

                                         ttGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                                             <  2:1630111‑M1/93‑37 (MQ=255)

                                           gAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggt                                           >  1:1322822‑M1/1‑55 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                          <  2:3966154‑M1/93‑40 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                          >  1:491904‑M1/1‑54 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                          >  1:1557030‑M1/1‑54 (MQ=255)

                                             aaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                                         <  1:2519292‑M1/93‑41 (MQ=255)

                                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                        <  2:4634425‑M1/93‑42 (MQ=255)

                                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                        >  2:2830877‑M1/1‑52 (MQ=255)

                                              aaaCCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                        <  1:2340293‑M1/93‑42 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                       >  1:5600717‑M1/1‑51 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                       <  2:5526666‑M1/93‑43 (MQ=255)

                                                aGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcggg                                      >  2:1732689‑M1/1‑50 (MQ=255)

                                                aGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcggg                                      >  1:2368202‑M1/1‑50 (MQ=255)

                                                 gCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACAGCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggt                                     >  1:4434490‑M1/1‑49 (MQ=255)

                                                   ggCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtta                                   >  2:6065978‑M1/1‑47 (MQ=255)

                                                   ggCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtta                                   <  1:5100499‑M1/93‑47 (MQ=255)

                                                   ggCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtta                                   >  2:3841353‑M1/1‑47 (MQ=255)

                                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                  <  2:1176781‑M1/93‑48 (MQ=255)

                                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                  <  1:5675066‑M1/93‑48 (MQ=255)

                                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagt                                 >  1:1070714‑M1/1‑45 (MQ=255)

                                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                                >  2:4244818‑M1/1‑44 (MQ=255)

                                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                                >  2:2932139‑M1/1‑44 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                               >  1:5598285‑M1/1‑43 (MQ=255)

                                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagc                            >  1:744552‑M1/1‑40 (MQ=255)

                                                           aTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcc                           <  2:5227511‑M1/93‑55 (MQ=255)

                                                             ttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccac                         <  1:3310987‑M1/93‑57 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                        <  1:869440‑M1/93‑58 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                       <  2:1720805‑M1/93‑59 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                       >  2:4193326‑M1/1‑35 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                       >  2:705490‑M1/1‑35 (MQ=255)

                                                                ccAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacagg                      <  1:322741‑M1/93‑60 (MQ=255)

                                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggc                     >  1:4665460‑M1/1‑33 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                    >  2:527818‑M1/1‑32 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                   <  1:1913529‑M1/93‑63 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                   >  1:5508875‑M1/1‑31 (MQ=255)

                                                                    ccGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc                  >  1:834835‑M1/1‑30 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                 >  2:790729‑M1/1‑29 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                <  1:1221936‑M1/93‑66 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                <  2:2399596‑M1/93‑66 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt               >  2:5844314‑M1/1‑27 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt               >  2:2629676‑M1/1‑27 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt               >  1:3625319‑M1/1‑27 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca             <  2:3109022‑M1/93‑69 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca             <  1:316357‑M1/93‑69 (MQ=255)

                                                                           ggAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagg           <  1:3785363‑M1/93‑71 (MQ=255)

                                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcaggg          >  1:5137328‑M1/1‑22 (MQ=255)

                                                                                tgatgaTTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatt      <  2:3341002‑M1/93‑76 (MQ=255)

                                                                                tgatgaTTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatt      >  1:6140995‑M1/1‑18 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta     <  1:4862364‑M1/93‑77 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta     <  2:1232764‑M1/93‑77 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta     <  1:1143395‑M1/93‑77 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag    <  2:4297577‑M1/93‑78 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag    <  1:1897570‑M1/93‑78 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt   <  2:1419829‑M1/93‑79 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt   <  1:3193782‑M1/93‑79 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt   <  2:2102003‑M1/93‑79 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt   >  2:3437393‑M1/1‑15 (MQ=255)

                                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtc  >  1:2184539‑M1/1‑14 (MQ=255)

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCC  >  NC_000913/3754220‑3754396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`