BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,806,223	A→G	I19M (ATA→ATG)	kdtA →	3‑deoxy‑D‑manno‑octulosonic‑acid transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,806,223	0	A	G	100.0%	760.9 / NA	234	I19M (ATA→ATG)	kdtA	3‑deoxy‑D‑manno‑octulosonic‑acid transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (153/81); total (153/81)

Only 100 of 237 total aligned reads displayed.

TCCCCAGCACATCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGG  >  NC_000913/3806135‑3806312

tCCCCAGCACATCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGc                                                                                       >  1:4755188/1‑93 (MQ=255)

  cccAGCACATCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGctc                                                                                     >  1:1169246/1‑93 (MQ=255)

   ccAGCACATCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGctct                                                                                    >  1:2300065/1‑93 (MQ=255)

    cAGCACATCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTCCCTTATTCAGCCGCTGATCTGGATGCGGCTCTg                                                                                   <  1:1051697/93‑1 (MQ=255)

       cacaTCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGt                                                                                >  2:870852/1‑93 (MQ=255)

          aTCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTgcg                                                                             >  1:2415509/1‑93 (MQ=255)

          aTCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACAGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTgcg                                                                             >  1:3391010/1‑93 (MQ=255)

           tCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTgcgc                                                                            <  2:421094/93‑1 (MQ=255)

           tCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGACGCTGATCTGGATGCGGCTCTGGGTgcgc                                                                            >  2:4502309/1‑93 (MQ=255)

           tCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACATTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTgcgc                                                                            >  1:5480553/1‑93 (MQ=255)

            ccATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGcgcg                                                                           >  2:2047755/1‑93 (MQ=255)

             cATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCgg                                                                          >  1:2549385/1‑93 (MQ=255)

              aTAAGTCAGCTATTTACTATGCTCGAATTGCTTTACCCCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGa                                                                         <  1:3585071/93‑1 (MQ=255)

               tAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGAc                                                                        <  2:6291399/93‑1 (MQ=255)

                aaGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACg                                                                       <  2:4394014/93‑1 (MQ=255)

                 aGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGt                                                                      <  2:5385528/93‑1 (MQ=255)

                 aGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGt                                                                      <  1:4510369/93‑1 (MQ=255)

                  gTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTa                                                                     >  2:1876704/1‑93 (MQ=255)

                       ttATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCt                                                                <  1:5846582/92‑1 (MQ=255)

                        tATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTc                                                               >  2:5796917/1‑93 (MQ=255)

                        tATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTc                                                               <  2:6055592/93‑1 (MQ=255)

                         aTTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTcc                                                              >  2:2183059/1‑93 (MQ=255)

                         aTTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTcc                                                              >  1:1218736/1‑93 (MQ=255)

                            tACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGc                                                           <  1:3691395/93‑1 (MQ=255)

                             aCTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGcc                                                          <  1:3912577/93‑1 (MQ=255)

                               tATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTa                                                        >  1:3249003/1‑93 (MQ=255)

                               tATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTa                                                        >  1:6242493/1‑93 (MQ=255)

                                  gCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCg                                                     >  2:1610624/1‑93 (MQ=255)

                                  gCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCg                                                     >  2:6187944/1‑93 (MQ=255)

                                   cTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGa                                                    >  2:2505223/1‑93 (MQ=255)

                                   cTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGa                                                    >  1:2992893/1‑93 (MQ=255)

                                   cTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGa                                                    >  2:5786445/1‑93 (MQ=255)

                                    tCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGaa                                                   >  1:2625070/1‑93 (MQ=255)

                                    tCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGaa                                                   <  1:5172744/93‑1 (MQ=255)

                                     cGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGaca                                                  >  1:1812527/1‑91 (MQ=255)

                                      gAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGaaaa                                                 >  2:2896516/1‑93 (MQ=255)

                                       aaTTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAAc                                                >  1:1606200/1‑93 (MQ=255)

                                       aaTTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAAc                                                >  2:3713726/1‑93 (MQ=255)

                                       aaTTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCCGCCTATCGAAAAc                                                >  2:4314693/1‑93 (MQ=255)

                                         ttGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGc                                              >  2:5657209/1‑93 (MQ=255)

                                         ttGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGc                                              <  1:5997306/93‑1 (MQ=255)

                                           tcTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTg                                            <  1:4673/92‑1 (MQ=255)

                                           gCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTg                                            <  2:4101116/93‑1 (MQ=255)

                                           gCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTg                                            <  1:3281139/93‑1 (MQ=255)

                                           gCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTg                                            <  1:948299/93‑1 (MQ=255)

                                            cTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTgg                                           <  1:2936984/93‑1 (MQ=255)

                                             tttACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTggg                                          <  2:1013973/93‑1 (MQ=255)

                                             tttACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTggg                                          >  2:2804416/1‑93 (MQ=255)

                                             tttACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTggg                                          >  1:5440036/1‑93 (MQ=255)

                                              ttACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTgggg                                         <  2:2421467/93‑1 (MQ=255)

                                                  acCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGaa                                     <  1:4841553/93‑1 (MQ=255)

                                                   ccGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAAc                                    >  1:891476/1‑93 (MQ=255)

                                                   ccGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAAc                                    >  1:1483340/1‑93 (MQ=255)

                                                    cGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACg                                   >  1:6246378/1‑93 (MQ=255)

                                                    cGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACg                                   <  2:4025432/93‑1 (MQ=255)

                                                      cccTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGtt                                 >  2:5238772/1‑93 (MQ=255)

                                                      cccTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGtt                                 >  2:78816/1‑93 (MQ=255)

                                                       ccTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTa                                >  2:1345631/1‑93 (MQ=255)

                                                        cTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTAc                               <  2:1739818/93‑1 (MQ=255)

                                                        cTTCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTAc                               >  2:3426277/1‑93 (MQ=255)

                                                         ttCTCTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACg                              >  2:4250612/1‑93 (MQ=255)

                                                          tctcTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACgg                             >  1:664280/1‑93 (MQ=255)

                                                          tctcTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACgg                             <  1:5085117/93‑1 (MQ=255)

                                                          tctcTACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACgg                             >  1:3738328/1‑93 (MQ=255)

                                                           ctctACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGt                            >  2:1432317/1‑93 (MQ=255)

                                                           ctctACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGt                            <  2:1936881/93‑1 (MQ=255)

                                                            tctACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGtt                           >  1:1959441/1‑93 (MQ=255)

                                                            tctACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGtt                           >  2:2291659/1‑93 (MQ=255)

                                                              tACCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGtttt                         <  2:4858378/93‑1 (MQ=255)

                                                               aCCTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGttttt                        <  2:5068616/93‑1 (MQ=255)

                                                                ccTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGtttttc                       >  1:4924780/1‑92 (MQ=255)

                                                                 cTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTAc                      >  2:1256315/1‑93 (MQ=255)

                                                                 cTTATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTAc                      >  1:3366440/1‑93 (MQ=255)

                                                                  ttATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTAcc                     >  1:3166627/1‑93 (MQ=255)

                                                                  ttATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTAcc                     >  1:6129877/1‑93 (MQ=255)

                                                                  ttATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTAcc                     <  1:4460426/93‑1 (MQ=255)

                                                                   tATTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCg                    <  1:265952/93‑1 (MQ=255)

                                                                    aTTCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGc                   >  2:5633750/1‑93 (MQ=255)

                                                                     ttCAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGAGTACGGTACGGTTTTTACCGcc                  >  1:4451523/1‑93 (MQ=255)

                                                                       cAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCAt                >  2:494259/1‑93 (MQ=255)

                                                                       cAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCAt                <  1:4878729/93‑1 (MQ=255)

                                                                       cAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCAt                >  1:5571238/1‑93 (MQ=255)

                                                                       cAGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCAt                >  1:3803667/1‑93 (MQ=255)

                                                                        aGCCGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATc               >  2:288079/1‑93 (MQ=255)

                                                                           cGCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGc            >  2:5890171/1‑93 (MQ=255)

                                                                            gCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCt           >  2:4655449/1‑93 (MQ=255)

                                                                            gCTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGGGAACGTTACGGGTTTTACCGCCATCCGCt           >  1:1407448/1‑93 (MQ=255)

                                                                            gCTGATCTGGATGCGGCTCTGGGTGCGCGGACGGAAGGCTCCGGCCTATCGAAAACGCTGCGGTGAACGTTACGGTTTTTACCGCCATCCGCt           >  2:3869902/1‑93 (MQ=255)

                                                                             cTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTa          >  2:5326084/1‑93 (MQ=255)

                                                                             cTGATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTa          >  1:3425701/1‑93 (MQ=255)

                                                                               gATCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTaaa        >  1:2041072/1‑93 (MQ=255)

                                                                                aTCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTaaaa       <  2:255182/93‑1 (MQ=255)

                                                                                aTCTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTaaaa       >  2:3149160/1‑93 (MQ=255)

                                                                                  cTGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAAcc     >  1:5921977/1‑93 (MQ=255)

                                                                                   tGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAACCGCTGGGGTGACCGTTACGGTTTTTACCGCCATCCGCTAAAACCa    <  1:3996734/93‑1 (MQ=255)

                                                                                   tGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCa    >  1:3937788/1‑93 (MQ=255)

                                                                                   tGGATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCa    <  2:3670985/93‑1 (MQ=255)

                                                                                    ggATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAg   <  1:4143416/93‑1 (MQ=255)

                                                                                    ggATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAg   <  1:580444/93‑1 (MQ=255)

                                                                                     gATGCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATACGCTAAAACCAgg  >  1:5243695/1‑93 (MQ=255)

TCCCCAGCACATCCATAAGTCAGCTATTTACTATGCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGG  >  NC_000913/3806135‑3806312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`