BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	54,157	C→T	P182P (CCG→CCA)	surA ←	survival protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	54,157	0	C	T	99.6%	920.1 / NA	250	P182P (CCG→CCA)	surA	survival protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (139/110); minor base G (1/0); total (140/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

GAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAG  >  NC_000913/54067‑54246

 aaaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTggg                                                                                        >  2:3931691/1‑93 (MQ=255)

  aaTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGa                                                                                       <  2:4914166/93‑1 (MQ=255)

   aTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGAt                                                                                      >  2:331614/1‑93 (MQ=255)

   aTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGAt                                                                                      >  2:791094/1‑93 (MQ=255)

   aTCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGAt                                                                                      <  1:2936638/93‑1 (MQ=255)

    tCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATc                                                                                     <  2:5075965/93‑1 (MQ=255)

    tCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATc                                                                                     >  2:2874581/1‑93 (MQ=255)

       gcgcCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAgg                                                                                  >  1:652861/1‑93 (MQ=255)

       gcgcCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAgg                                                                                  >  1:5339311/1‑93 (MQ=255)

         gcCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAt                                                                                <  1:525445/93‑1 (MQ=255)

          ccGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtg                                                                               >  2:5474827/1‑93 (MQ=255)

           cGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtgt                                                                              <  1:2119729/93‑1 (MQ=255)

            gtgaCGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCCTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtgtg                                                                             <  2:4341763/90‑1 (MQ=255)

            gTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAtgtg                                                                             <  2:3836890/93‑1 (MQ=255)

              tACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGc                                                                           >  2:1096133/1‑93 (MQ=255)

              tACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGc                                                                           <  2:2094049/93‑1 (MQ=255)

               aCGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCt                                                                          >  1:510528/1‑93 (MQ=255)

               aCGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCt                                                                          <  1:2004613/93‑1 (MQ=255)

                 gcgcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCa                                                                        >  1:894844/1‑93 (MQ=255)

                  cgcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAg                                                                       <  2:1264816/93‑1 (MQ=255)

                  cgcCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAg                                                                       <  2:477685/93‑1 (MQ=255)

                  cgcCTGATCGACAATGGCGCGCGCCTGGCTGTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAg                                                                       <  1:2479373/93‑1 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  2:4075415/1‑93 (MQ=255)

                    ccTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGt                                                                     >  2:2929971/1‑93 (MQ=255)

                      tGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTc                                                                   >  1:1673952/1‑93 (MQ=255)

                       gATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCa                                                                  <  1:2152641/93‑1 (MQ=255)

                       gATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCa                                                                  >  2:3126104/1‑93 (MQ=255)

                        aTCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAg                                                                 <  2:3978603/93‑1 (MQ=255)

                          cGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCt                                                               <  1:5016369/93‑1 (MQ=255)

                           gACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTc                                                              >  1:4202838/1‑93 (MQ=255)

                            aCAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCa                                                             >  2:3444349/1‑93 (MQ=255)

                            aCAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCa                                                             >  2:4677539/1‑93 (MQ=255)

                             cAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAg                                                            >  1:2747382/1‑93 (MQ=255)

                              aaTGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGt                                                           >  1:304567/1‑93 (MQ=255)

                               aTGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTg                                                          >  2:2253986/1‑93 (MQ=255)

                               aTGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTg                                                          <  2:5925196/93‑1 (MQ=255)

                                 ggCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCt                                                        >  1:629043/1‑93 (MQ=255)

                                 ggCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCt                                                        <  2:1816447/93‑1 (MQ=255)

                                  gcgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTg                                                       <  2:4600838/93‑1 (MQ=255)

                                  gcgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTg                                                       <  1:1392987/93‑1 (MQ=255)

                                   cgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTgg                                                      >  2:2552828/1‑93 (MQ=255)

                                   cgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTCGCTCAGGTTCAGCTCAGTGCTgg                                                      <  1:5921864/93‑1 (MQ=255)

                                    gcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGc                                                     <  2:5960549/93‑1 (MQ=255)

                                     cgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGAt                                                                                >  1:4380868/1‑65 (MQ=255)

                                        gcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgtcg                                                 >  1:1245686/1‑93 (MQ=255)

                                        gcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgtcg                                                 >  1:2409572/1‑93 (MQ=255)

                                         ccTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgtcgt                                                >  1:585038/1‑93 (MQ=255)

                                          cTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtt                                               <  2:141123/93‑1 (MQ=255)

                                          cTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtt                                               >  2:2626956/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  1:128271/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  2:3890222/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  2:5519092/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  2:5001862/1‑93 (MQ=255)

                                           tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt                                              >  1:5384196/1‑93 (MQ=255)

                                            ggCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCa                                                             >  2:3437992/1‑77 (MQ=255)

                                            ggCTTTCCGCTTCGTTCACATGATCAGAGGTCGGATTTTCCGGCTGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCAGGCGTCGtttt                                             <  2:6297676/93‑1 (MQ=255)

                                             gCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTg                                            <  2:2714502/93‑1 (MQ=255)

                                                ttCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGtt                                         <  2:4422650/93‑1 (MQ=255)

                                                   cGCTTCGTTCACCTGATCAGAGGTCGGGTTTGCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAcc                                      <  1:4423906/93‑1 (MQ=255)

                                                     cTTCGTTCCCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa                                    >  2:430822/1‑93 (MQ=255)

                                                     cTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa                                    >  1:1557675/1‑93 (MQ=255)

                                                     cTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa                                    <  1:3557383/93‑1 (MQ=255)

                                                      ttCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc                                   >  1:2788367/1‑93 (MQ=255)

                                                      ttCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc                                   >  1:4643840/1‑93 (MQ=255)

                                                       tCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCagc                                  <  1:2233013/93‑3 (MQ=255)

                                                        cGTTCACCTGATCAGAGGTCGGCTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACct                                 <  2:1452669/93‑1 (MQ=255)

                                                          ttCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgc                               >  1:3887959/1‑93 (MQ=255)

                                                           tCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCCCctgct                              <  2:2790595/93‑1 (MQ=255)

                                                           tCACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgct                              >  2:3496635/1‑93 (MQ=255)

                                                            cACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgctg                             >  2:523725/1‑93 (MQ=255)

                                                            cACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgctg                             >  1:1438963/1‑93 (MQ=255)

                                                            cACCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgctg                             >  1:2304731/1‑93 (MQ=255)

                                                             aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            <  2:238181/93‑1 (MQ=255)

                                                             aCCTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCtgctgc                            >  1:3395385/1‑93 (MQ=255)

                                                              ccTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTgcg                           >  1:5410430/1‑93 (MQ=255)

                                                               cTGATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTgcgc                          >  1:3137771/1‑93 (MQ=255)

                                                                taaTCAGAGGTCGGGTTTGCCGGCAGTTGGATCAGTATGTGGCTCAGGTTCACCTCAGTGCTGGCGTCGTTTTGGTTATCCACCTGCTGCGcc                         <  1:3464173/91‑1 (MQ=255)

                                                                 gATCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCa                        >  2:3768446/1‑93 (MQ=255)

                                                                  aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       >  2:567753/1‑93 (MQ=255)

                                                                  aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       <  2:608236/93‑1 (MQ=255)

                                                                  aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       <  1:3594764/93‑1 (MQ=255)

                                                                  aTCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAg                       <  2:198210/93‑1 (MQ=255)

                                                                   tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAgg                      <  2:600814/93‑1 (MQ=255)

                                                                   tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAgg                      <  2:2474196/93‑1 (MQ=255)

                                                                   tCAGAGGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAgg                      <  2:577195/93‑1 (MQ=255)

                                                                     agagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGa                    <  1:1047091/93‑1 (MQ=255)

                                                                     agagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGa                    >  1:6072863/1‑93 (MQ=255)

                                                                      gagGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGAt                   <  1:2533622/93‑1 (MQ=255)

                                                                       agttCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGAtt                  <  2:2390651/90‑1 (MQ=255)

                                                                       agGTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGAtt                  >  1:3992285/1‑93 (MQ=255)

                                                                        ggTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTc                 >  2:2227553/1‑93 (MQ=255)

                                                                         gTCGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCg                >  2:2961157/1‑93 (MQ=255)

                                                                           cGGGTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGAc              >  2:4524906/1‑93 (MQ=255)

                                                                            gggTTTTCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACt             <  2:1658376/93‑1 (MQ=255)

                                                                               ttttCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTcc          <  2:1752225/93‑1 (MQ=255)

                                                                                  tCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGc       <  2:3210756/93‑1 (MQ=255)

                                                                                  tCCGGCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGc       <  2:939556/93‑1 (MQ=255)

                                                                                     ggCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGc    >  2:1322200/1‑93 (MQ=255)

                                                                                     ggCAGGGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCCGCGGc    >  1:316236/1‑93 (MQ=255)

                                                                                      gCAGTGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCa   >  1:4760739/1‑93 (MQ=255)

GAAATCAGCGCCGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAG  >  NC_000913/54067‑54246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`