BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,829,419	C→T	R446C (CGC→TGC)	yicH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,829,419	0	C	T	99.6%	845.7 / NA	231	R446C (CGC→TGC)	yicH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (91/139); minor base G (0/1); total (91/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

ACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCAC  >  NC_000913/3829329‑3829507

aCGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGGCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGc                                                                                        >  1:4999324/1‑93 (MQ=255)

  gCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGccc                                                                                      <  2:2260206/93‑1 (MQ=255)

  gCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGccc                                                                                      <  2:5650899/93‑1 (MQ=255)

   cTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGGCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCg                                                                                     >  1:1032180/1‑93 (MQ=255)

   cTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCg                                                                                     <  2:4451641/93‑1 (MQ=255)

    tGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTTGTTGCCCGt                                                                                    >  2:3479583/1‑93 (MQ=255)

     ggTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTc                                                                                   >  1:5092041/1‑93 (MQ=255)

      gTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCg                                                                                  <  2:3688459/93‑1 (MQ=255)

       ttACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGc                                                                                 >  1:934832/1‑93 (MQ=255)

        tACCGATCATAAATGGGGCGTGTGGAGTGGCTCCGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCt                                                                                >  2:4006914/1‑93 (MQ=255)

          ccGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTgg                                                                              <  2:6236472/93‑1 (MQ=255)

          ccGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTgg                                                                              >  1:4934297/1‑93 (MQ=255)

            gATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGgcg                                                                            >  1:1473795/1‑93 (MQ=255)

            gATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGgcg                                                                            >  1:1390208/1‑93 (MQ=255)

             aTCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGgcgc                                                                           <  2:5773397/93‑1 (MQ=255)

              tCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTTGTTGCCCGTGCCTGGCGCt                                                                          >  2:5107555/1‑93 (MQ=255)

              tCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCt                                                                          <  2:2740713/93‑1 (MQ=255)

                 tAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGAc                                                                       <  2:873356/93‑1 (MQ=255)

                     tGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGcc                                                                   <  1:852329/93‑1 (MQ=255)

                       gggCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTAAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCaa                                                                 >  1:5680995/1‑93 (MQ=255)

                         gCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAAca                                                               <  1:5319073/93‑1 (MQ=255)

                         gCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAAca                                                               >  1:6084121/1‑93 (MQ=255)

                         gCGTCTGGAGTGGCTCGGCGAATCGGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAAca                                                               <  1:3732109/93‑1 (MQ=255)

                             cTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAAcagcag                                                           <  1:3902585/93‑1 (MQ=255)

                             cTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAAcagcag                                                           <  1:4045804/93‑1 (MQ=255)

                              tGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACagcagc                                                          <  2:5576336/93‑1 (MQ=255)

                               ggAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAgcagca                                                         <  1:1888804/93‑1 (MQ=255)

                               ggAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAgcagca                                                         >  1:3259667/1‑93 (MQ=255)

                               ggAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAgcagca                                                         <  1:2222351/93‑1 (MQ=255)

                                gAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGGAGCAc                                                        >  1:1251530/1‑93 (MQ=255)

                                gAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCAc                                                        <  2:1703501/93‑1 (MQ=255)

                                  gTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACgg                                                      <  1:3525840/93‑1 (MQ=255)

                                    ggCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGCCCGCCAACAGCAGCACGGTg                                                    <  1:1190218/93‑1 (MQ=255)

                                      cTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGaa                                                  <  2:2287305/93‑1 (MQ=255)

                                       tCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAat                                                 >  1:5135839/1‑93 (MQ=255)

                                       tCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAat                                                 >  1:1070373/1‑93 (MQ=255)

                                        cGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAata                                                <  2:2782689/93‑1 (MQ=255)

                                        cGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAata                                                <  1:654323/93‑1 (MQ=255)

                                          gCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATc                                              <  2:2073095/93‑1 (MQ=255)

                                            gAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAg                                            >  2:3559641/1‑93 (MQ=255)

                                            gAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAg                                            <  1:5734564/93‑1 (MQ=255)

                                             aaacTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGc                                           <  2:3910165/90‑1 (MQ=255)

                                              aTCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCg                                          <  2:5923486/93‑1 (MQ=255)

                                              aTCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCg                                          <  2:3777303/93‑1 (MQ=255)

                                               tCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGa                                         >  1:4229134/1‑93 (MQ=255)

                                               tCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGa                                         >  2:5253342/1‑93 (MQ=255)

                                               tCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGa                                         >  1:6239199/1‑93 (MQ=255)

                                               tCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGa                                         >  1:4391275/1‑93 (MQ=255)

                                                cTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGaa                                        <  2:4704663/93‑1 (MQ=255)

                                                 tGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAAc                                       <  1:2838661/93‑1 (MQ=255)

                                                 tGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAAc                                       <  2:4333738/93‑1 (MQ=255)

                                                  gAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACt                                      >  1:5584203/1‑93 (MQ=255)

                                                   aaTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTg                                     <  1:3047204/93‑1 (MQ=255)

                                                    aTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGa                                    <  2:263074/93‑1 (MQ=255)

                                                     tGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAg                                   <  2:788908/93‑1 (MQ=255)

                                                     tGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAg                                   >  2:5393461/1‑93 (MQ=255)

                                                      gccgccgccGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGt                                  <  1:1527937/93‑1 (MQ=255)

                                                      gccgccgccGCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGt                                  >  2:1869764/1‑93 (MQ=255)

                                                       ccgccgccgCGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTg                                 >  2:4822059/1‑93 (MQ=255)

                                                            gccgcGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCAttt                            >  2:1010854/1‑93 (MQ=255)

                                                            gccgcGACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCAttt                            >  2:4713974/1‑93 (MQ=255)

                                                            gccgcGACATTCAAGCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCAttt                            >  2:3850686/1‑93 (MQ=255)

                                                               gcgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACt                         >  1:409584/1‑93 (MQ=255)

                                                               gcgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACt                         >  2:744698/1‑93 (MQ=255)

                                                               gcgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACt                         >  2:296035/1‑93 (MQ=255)

                                                               gcgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACt                         >  2:1421020/1‑93 (MQ=255)

                                                               gcgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACt                         >  1:1686504/1‑93 (MQ=255)

                                                                cgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTg                        <  2:3058702/93‑1 (MQ=255)

                                                                cgACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTg                        <  1:1962615/93‑1 (MQ=255)

                                                                 gACATTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGACAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGa                       >  2:1724408/1‑93 (MQ=255)

                                                                    aTTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaa                    >  1:4477457/1‑93 (MQ=255)

                                                                    aTTCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGGGAATATCAGCGAACTGAGTGCATTTACTGaaga                    >  2:1120659/1‑91 (MQ=255)

                                                                     ttCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaag                   >  1:2585229/1‑92 (MQ=255)

                                                                     ttCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaaa                   <  2:2155411/93‑1 (MQ=255)

                                                                     ttCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaaa                   <  1:2661116/93‑1 (MQ=255)

                                                                     ttCAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaaa                   <  2:4950418/93‑1 (MQ=255)

                                                                       cAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAgg                 <  2:6207084/93‑1 (MQ=255)

                                                                       cAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAgg                 <  2:2401155/93‑1 (MQ=255)

                                                                       cAATCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGCTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAgg                 >  2:3284303/1‑93 (MQ=255)

                                                                        aaTCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGc                <  1:47541/93‑1 (MQ=255)

                                                                        aaTCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGc                <  1:4819526/93‑1 (MQ=255)

                                                                         aTCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCa               <  2:1588274/93‑1 (MQ=255)

                                                                         aTCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCa               <  2:2490535/93‑1 (MQ=255)

                                                                          tCGTGTTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCAt              <  1:2000109/93‑1 (MQ=255)

                                                                            gtgtTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTc            >  2:5339694/1‑93 (MQ=255)

                                                                            gtgtTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTc            <  1:3176836/93‑1 (MQ=255)

                                                                             tgtTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCt           <  1:3695910/93‑1 (MQ=255)

                                                                             tgtTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCt           <  1:265043/93‑1 (MQ=255)

                                                                             tgtTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCt           <  2:479328/93‑1 (MQ=255)

                                                                              gtTGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTg          <  1:527953/93‑1 (MQ=255)

                                                                               ttGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTgg         <  1:1743519/93‑1 (MQ=255)

                                                                                tGATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGa        <  1:2123223/93‑1 (MQ=255)

                                                                                tGATCTTCGGTGCCCGTCGCCGGCGCTGACCGCCACCAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACAGAAAAAGGCATTCTGGa        >  2:544007/1‑93 (MQ=255)

                                                                                 gATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGaa       <  2:5002629/93‑1 (MQ=255)

                                                                                 gATGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGaa       <  2:6061940/93‑1 (MQ=255)

                                                                                   tGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGc     <  2:3108333/93‑1 (MQ=255)

                                                                                   tGTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGc     <  1:2454071/93‑1 (MQ=255)

                                                                                    gTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGcc    >  2:1757128/1‑93 (MQ=255)

                                                                                    gTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGcc    <  2:1616404/93‑1 (MQ=255)

                                                                                    gTTCGTTGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGcc    <  1:4236260/93‑1 (MQ=255)

ACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCAC  >  NC_000913/3829329‑3829507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`