Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 435,927 A→G T39A (ACG→GCG)  pgpA → phosphatidylglycerophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913435,9270AG99.5% 667.9 / NA 208T39A (ACG→GCG) pgpAphosphatidylglycerophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (99/108);  minor base T (0/1);  total (99/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATC  >  NC_000913/435837‑436016
                                                                                          |                                                                                         
gATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCg                                                                                         >  2:3668935/1‑93 (MQ=255)
   gTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATg                                                                                      <  1:2031065/93‑1 (MQ=255)
   gTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATg                                                                                      <  1:2113909/93‑1 (MQ=255)
    tCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATgg                                                                                     <  1:4474982/93‑1 (MQ=255)
    tCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATgg                                                                                     >  1:6190074/1‑93 (MQ=255)
    tCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATgg                                                                                     >  2:558276/1‑93 (MQ=255)
      gcgAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGc                                                                                   <  1:1662170/93‑1 (MQ=255)
       cgAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCt                                                                                  >  2:1390406/1‑93 (MQ=255)
          aaaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGc                                                                               <  2:1694120/93‑1 (MQ=255)
           aaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCt                                                                              <  1:5522772/93‑1 (MQ=255)
           aaGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCt                                                                              >  2:1822244/1‑93 (MQ=255)
              tCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGc                                                                           <  2:5062193/93‑1 (MQ=255)
               cGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCa                                                                          <  1:5326296/93‑1 (MQ=255)
               cGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCa                                                                          <  2:5300793/93‑1 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAg                                                                         >  2:3529259/1‑93 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAg                                                                         <  1:5999451/93‑1 (MQ=255)
                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAg                                                                         >  1:4643372/1‑93 (MQ=255)
                 ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGc                                                                        <  2:4783857/93‑1 (MQ=255)
                 ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGc                                                                        <  1:414299/93‑1 (MQ=255)
                 ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGc                                                                        >  2:3180417/1‑93 (MQ=255)
                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTCAGCCCGCTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGa                                                                      >  2:3283515/1‑93 (MQ=255)
                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGa                                                                      >  2:2822802/1‑93 (MQ=255)
                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGa                                                                      >  2:571562/1‑93 (MQ=255)
                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGa                                                                      >  2:5528132/1‑93 (MQ=255)
                      aGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGTAGCGATTc                                                                   <  2:6142401/93‑1 (MQ=255)
                      aGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTc                                                                   >  1:2436345/1‑93 (MQ=255)
                      aGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTc                                                                   <  2:2934522/93‑1 (MQ=255)
                       gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTcc                                                                  >  1:4188663/1‑93 (MQ=255)
                         tGAGTAATCCGTGGCATCTACGTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGt                                                                <  1:3077998/93‑1 (MQ=255)
                          gAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGtt                                                               >  2:5881127/1‑93 (MQ=255)
                          gAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGtt                                                               >  1:5500457/1‑93 (MQ=255)
                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCt                                                             <  1:4292316/93‑1 (MQ=255)
                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCt                                                             >  1:1156592/1‑93 (MQ=255)
                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCt                                                             <  2:1521441/93‑1 (MQ=255)
                               aTCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGt                                                          >  2:6228615/1‑93 (MQ=255)
                               aTCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGt                                                          >  1:77124/1‑93 (MQ=255)
                                 ccGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTAt                                                        <  1:3767674/93‑1 (MQ=255)
                                 ccGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTAt                                                        <  1:3428753/93‑1 (MQ=255)
                                  cGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATc                                                       <  1:1049920/93‑1 (MQ=255)
                                   gTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCt                                                      >  2:4480304/1‑93 (MQ=255)
                                    tGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCtg                                                     <  2:569846/93‑1 (MQ=255)
                                    tGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGGTCCTTGGGCGATGGGCTCGCTGGCAGCGCTTCCTGTTTGGTATCtg                                                     >  2:1653330/1‑93 (MQ=255)
                                     ggCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCCGCGATTCCGTTCTGGTATCtga                                                    >  1:3476722/1‑93 (MQ=255)
                                         tCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAc                                                >  1:1731118/1‑93 (MQ=255)
                                         tCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAc                                                >  1:1401476/1‑93 (MQ=255)
                                         tCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAc                                                <  2:4350895/93‑1 (MQ=255)
                                         acTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAc                                                >  2:2706118/2‑93 (MQ=255)
                                          cTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAcc                                               <  2:1777284/93‑1 (MQ=255)
                                           tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCt                                              <  1:6215363/93‑1 (MQ=255)
                                           tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCt                                              <  1:5587518/93‑1 (MQ=255)
                                             cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                            >  2:4417984/1‑93 (MQ=255)
                                             cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                            <  1:4890690/93‑1 (MQ=255)
                                             cTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                            <  1:2309793/93‑1 (MQ=255)
                                              ttGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                           <  2:2368697/93‑1 (MQ=255)
                                               tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttttt                                          <  2:799913/93‑1 (MQ=255)
                                               tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttttt                                          <  1:5915661/93‑1 (MQ=255)
                                                gCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                         >  2:4065107/1‑93 (MQ=255)
                                                  tGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGcc                                       <  2:1164579/93‑1 (MQ=255)
                                                   gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGccc                                      <  1:5201729/93‑1 (MQ=255)
                                                    tCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                     >  1:286101/1‑93 (MQ=255)
                                                     cGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTg                                    <  1:4322458/93‑1 (MQ=255)
                                                       gATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGc                                  >  1:4999972/1‑93 (MQ=255)
                                                        aTTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCa                                 >  2:6210905/1‑93 (MQ=255)
                                                        aTTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCa                                 >  1:1310109/1‑93 (MQ=255)
                                                          tCGGAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGc                               <  1:2405010/93‑1 (MQ=255)
                                                             gAAGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctct                            >  1:597148/1‑93 (MQ=255)
                                                              aaGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTa                           >  1:1098299/1‑93 (MQ=255)
                                                               aGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAc                          >  1:4257548/1‑93 (MQ=255)
                                                               aGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAc                          >  1:2926865/1‑93 (MQ=255)
                                                               aGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAc                          <  2:2643355/93‑1 (MQ=255)
                                                               aGTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAc                          <  1:5737544/93‑1 (MQ=255)
                                                                gTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTTATGACCTTTTTGCCCTGGCAGCTCTACt                         <  2:702808/93‑1 (MQ=255)
                                                                gTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACt                         <  1:3982019/93‑1 (MQ=255)
                                                                gTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACt                         >  2:5249810/1‑93 (MQ=255)
                                                                gTGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCCGGCAGCTCTACt                         <  1:3403743/93‑1 (MQ=255)
                                                                 tGGATTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTc                        <  2:4061237/93‑1 (MQ=255)
                                                                    aTTAAGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCt                     <  1:881427/93‑1 (MQ=255)
                                                                       aaGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggg                  >  1:331844/1‑92 (MQ=255)
                                                                        aGCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtg                 <  2:2439208/93‑1 (MQ=255)
                                                                         gCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                >  2:3959971/1‑93 (MQ=255)
                                                                         gCCCGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                >  1:2806837/1‑93 (MQ=255)
                                                                            cGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGgtggtgg             >  2:2570859/1‑92 (MQ=255)
                                                                            cGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGa             <  2:4464053/93‑1 (MQ=255)
                                                                            cGATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGa             <  1:1944198/93‑1 (MQ=255)
                                                                             gATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt            <  2:1624521/93‑1 (MQ=255)
                                                                             gATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt            >  2:1886615/1‑93 (MQ=255)
                                                                             gATCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGGGAt            >  1:2498136/1‑93 (MQ=255)
                                                                              aTCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATg           >  1:168539/1‑93 (MQ=255)
                                                                               tCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGc          >  2:1349979/1‑93 (MQ=255)
                                                                               tCGTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGc          >  2:2808083/1‑93 (MQ=255)
                                                                                 gTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTg        >  1:4767342/1‑93 (MQ=255)
                                                                                 gTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTg        <  1:4737523/93‑1 (MQ=255)
                                                                                 gTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTg        <  1:6257515/93‑1 (MQ=255)
                                                                                 gTTCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGAAGCTCTACTCGCTGGTGGTGAGGCTg        >  1:1980951/1‑93 (MQ=255)
                                                                                  ttCCTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgg       <  1:6035266/93‑1 (MQ=255)
                                                                                  ttCCTGGGGCGATGGGCTCGCGATAAGAGATTCAGTTCTTATATCTGATGACCATTTTGGCCTAGCAGCTCTACTCGCTGGTGGTGatgatgt       <  2:1457451/93‑2 (MQ=255)
                                                                                    ccTGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTgggg     <  1:1233035/93‑1 (MQ=255)
                                                                                      tGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGAt   <  2:458781/93‑1 (MQ=255)
                                                                                      tGGGGCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGAt   <  2:420175/93‑1 (MQ=255)
                                                                                       ggggCGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc  <  2:4796351/93‑1 (MQ=255)
                                                                                          |                                                                                         
GATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATC  >  NC_000913/435837‑436016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑