BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	436,659	T→C	E258G (GAG→GGG)	yajO ←	putative NAD(P)H‑dependent xylose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,659	0	T	C	100.0%	784.1 / NA	240	E258G (GAG→GGG)	yajO	putative NAD(P)H‑dependent xylose reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (140/100); total (140/100)

Only 100 of 254 total aligned reads displayed.

GTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCC  >  NC_000913/436569‑436748

  cGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCccatc                                                                                       <  2:2137905/93‑1 (MQ=255)

      cccAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCACCACCCCATCAGac                                                                                   >  1:2695445/1‑93 (MQ=255)

       ccAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGaca                                                                                  >  2:859391/1‑93 (MQ=255)

       ccAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGaca                                                                                  >  1:758039/1‑93 (MQ=255)

         aGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACAcc                                                                                <  2:107280/93‑1 (MQ=255)

         aGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCAGCAGACAcc                                                                                <  2:6116915/93‑1 (MQ=255)

          gTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCa                                                                               <  2:64602/93‑1 (MQ=255)

          gTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCa                                                                               <  2:5151254/93‑1 (MQ=255)

          gTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCa                                                                               <  2:469684/93‑1 (MQ=255)

          gTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCa                                                                               <  1:2095019/93‑1 (MQ=255)

           gtcttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAg                                                                              <  1:1179345/92‑1 (MQ=255)

            tcttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGt                                                                             <  1:570194/93‑1 (MQ=255)

             cttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCCCCCCATCAGACACCAGTc                                                                            <  1:1593208/93‑1 (MQ=255)

              ttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCg                                                                           >  1:2627382/1‑93 (MQ=255)

               tcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGt                                                                          >  1:4864465/1‑93 (MQ=255)

               tcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGt                                                                          <  2:1279387/93‑1 (MQ=255)

                cACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTg                                                                         <  2:3383853/93‑1 (MQ=255)

                cACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTg                                                                         >  2:2842955/1‑93 (MQ=255)

                 aCTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGc                                                                        >  1:1549742/1‑93 (MQ=255)

                   tGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCgg                                                                      <  1:472840/93‑1 (MQ=255)

                   tGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCgg                                                                      <  2:5627406/93‑1 (MQ=255)

                     aCGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTa                                                                    <  1:6172921/93‑1 (MQ=255)

                       gCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGt                                                                  <  2:1541304/93‑1 (MQ=255)

                        ccTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGtt                                                                 >  2:2585827/1‑93 (MQ=255)

                        ccTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGtt                                                                 >  1:1842276/1‑93 (MQ=255)

                         cTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGttt                                                                <  1:6148604/93‑1 (MQ=255)

                         cTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGttt                                                                >  1:5198539/1‑93 (MQ=255)

                          tGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtc                                                               >  1:5179459/1‑93 (MQ=255)

                          tGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtc                                                               >  2:105910/1‑93 (MQ=255)

                          tGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtc                                                               <  1:2264896/93‑1 (MQ=255)

                           gTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATTAGACACCAGTCGTGCGGTAGTTtct                                                              >  1:4112959/1‑93 (MQ=255)

                           gTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtct                                                              >  1:1272735/1‑93 (MQ=255)

                           gTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtct                                                              >  1:1780200/1‑93 (MQ=255)

                              aaCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTccc                                                           >  1:1000730/1‑93 (MQ=255)

                                 cGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCAc                                                        >  2:1966185/1‑93 (MQ=255)

                                 cGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCAc                                                        <  2:55120/93‑1 (MQ=255)

                                  gCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACg                                                       <  2:5940493/93‑1 (MQ=255)

                                    tctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGa                                                     >  1:1410418/1‑93 (MQ=255)

                                     ctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGAc                                                    <  2:3895344/93‑1 (MQ=255)

                                      tGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGAcg                                                   >  2:5751703/1‑93 (MQ=255)

                                      tGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGAcg                                                   >  1:4687386/1‑93 (MQ=255)

                                          aTCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTc                                               <  1:2740932/93‑1 (MQ=255)

                                          aTCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTc                                               <  2:5260705/93‑1 (MQ=255)

                                           tCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCa                                              >  2:4509432/1‑93 (MQ=255)

                                             tGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGa                                            >  2:3719079/1‑93 (MQ=255)

                                              gcgcGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGAc                                           <  2:5039813/93‑1 (MQ=255)

                                                gcgTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACACCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACgg                                         >  2:5198516/1‑93 (MQ=255)

                                                 cgTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGc                                        <  2:557826/93‑1 (MQ=255)

                                                  gTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcc                                       >  1:5432846/1‑93 (MQ=255)

                                                   tCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGccc                                      >  1:3306963/1‑93 (MQ=255)

                                                   tCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGccc                                      >  2:3232870/1‑93 (MQ=255)

                                                   tCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGccc                                      >  1:5324491/1‑93 (MQ=255)

                                                   tCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGccc                                      >  1:1156079/1‑93 (MQ=255)

                                                    cATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcccc                                     >  1:787441/1‑93 (MQ=255)

                                                    cATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcccc                                     <  1:3480143/93‑1 (MQ=255)

                                                    cATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcccc                                     >  1:4426998/1‑93 (MQ=255)

                                                     aTTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCt                                    >  2:4838225/1‑93 (MQ=255)

                                                     aTTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCt                                    >  1:5499968/1‑93 (MQ=255)

                                                     aTTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCt                                    <  2:244449/93‑1 (MQ=255)

                                                      ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                   >  2:2519731/1‑93 (MQ=255)

                                                      ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                   >  1:1862227/1‑93 (MQ=255)

                                                        ttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGc                                 <  1:2884813/93‑1 (MQ=255)

                                                         tCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGcc                                >  1:3574512/1‑93 (MQ=255)

                                                          cATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCa                               <  2:5902185/93‑1 (MQ=255)

                                                          cATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCa                               >  1:4176787/1‑93 (MQ=255)

                                                              gCTTTCTTTATAGATATGTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCgg                           <  2:4362316/93‑1 (MQ=255)

                                                              gCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGGCGGCCCCTTGCCAGCgg                           <  2:1751560/93‑1 (MQ=255)

                                                              gCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCgg                           >  2:5338892/1‑93 (MQ=255)

                                                              gCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCgg                           >  1:5399683/1‑93 (MQ=255)

                                                                tttctttATAGAGATTTTCCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCGCTTGCCAGGGGGc                         <  2:1921178/93‑1 (MQ=255)

                                                                  tctttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTc                       >  2:4646871/1‑93 (MQ=255)

                                                                  tctttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTc                       >  2:4099430/1‑93 (MQ=255)

                                                                   ctttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGANGGCCCCTTGCCAGCGGGCTcc                      >  1:2559293/1‑93 (MQ=255)

                                                                   ctttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTcc                      >  1:23131/1‑93 (MQ=255)

                                                                     ttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCAt                    <  2:1179947/93‑1 (MQ=255)

                                                                      tataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATg                   >  2:1415663/1‑93 (MQ=255)

                                                                      tataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATg                   >  2:1655872/1‑93 (MQ=255)

                                                                       ataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATgg                  <  1:509548/93‑1 (MQ=255)

                                                                        taGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGGCGGCACCTTGCCAGCGGGCTCCATGGa                 >  2:2903019/1‑93 (MQ=255)

                                                                        taGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGACCCTTGCAAGCGGGCTCCATGGa                 >  2:3006869/1‑93 (MQ=255)

                                                                         agagATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGaa                >  2:3529557/1‑93 (MQ=255)

                                                                          gagaTTTTTCCCCCCCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAAt               <  2:6282202/93‑1 (MQ=255)

                                                                           agaTTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAAtt              <  2:595638/93‑1 (MQ=255)

                                                                             aTTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAc            <  1:4526965/93‑1 (MQ=255)

                                                                             aTTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAc            >  1:6042480/1‑93 (MQ=255)

                                                                             aTTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAc            >  1:3033999/1‑93 (MQ=255)

                                                                              tttttCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAcc           >  2:6152846/1‑93 (MQ=255)

                                                                                 ttCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc        >  1:1072590/1‑93 (MQ=255)

                                                                                  tCCCCCCCCCATCAGACCCCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCa       <  1:290966/93‑1 (MQ=255)

                                                                                  tCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCa       <  1:5572420/93‑1 (MQ=255)

                                                                                  tCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCa       >  1:6181608/1‑93 (MQ=255)

                                                                                   ccccaccccaTCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCAc      >  2:5451954/1‑93 (MQ=255)

                                                                                   ccccaccccaTCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCAc      <  2:6193315/93‑1 (MQ=255)

                                                                                   ccccaccccaTCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCAc      >  2:2402698/1‑93 (MQ=255)

                                                                                   ccccaccccaTCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCAc      <  2:2223586/93‑1 (MQ=255)

                                                                                    cccACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACg     <  2:3338151/93‑1 (MQ=255)

                                                                                      cACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGTCTCCATGGAATTACCGCCACGcc   <  1:699035/93‑1 (MQ=255)

                                                                                      cACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGcc   >  1:5595210/1‑93 (MQ=255)

                                                                                      cACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGcc   >  1:1647994/1‑93 (MQ=255)

                                                                                       aCCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGccc  >  1:4285195/1‑93 (MQ=255)

GTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCC  >  NC_000913/436569‑436748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`