Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,917,524 G→A R170C (CGC→TGC)  atpH ← membrane‑bound ATP synthase, F1 sector, delta‑subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,917,5240GA99.6% 990.3 / ‑5.6 273R170C (CGC→TGC) atpHmembrane‑bound ATP synthase, F1 sector, delta‑subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (131/141);  total (131/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 278 total aligned reads displayed.

GAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAAT  >  NC_000913/3917434‑3917613
                                                                                          |                                                                                         
gAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACt                                                                                         >  1:3181174/1‑93 (MQ=255)
 aGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTc                                                                                        >  1:1043443/1‑93 (MQ=255)
 aGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTc                                                                                        <  1:5074672/93‑1 (MQ=255)
    aaTGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAg                                                                                     >  2:4303826/1‑93 (MQ=255)
     aTGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAga                                                                                    <  2:4296862/93‑1 (MQ=255)
        cgcTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAgacga                                                                                 <  2:1071610/93‑1 (MQ=255)
         gctgctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAgacgac                                                                                <  2:3014535/93‑1 (MQ=255)
          ctgctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGAcc                                                                               <  2:3496125/93‑1 (MQ=255)
          ctgctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGAcc                                                                               <  1:4384207/93‑1 (MQ=255)
            gctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACcgc                                                                             >  2:5376391/1‑93 (MQ=255)
            gctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACcgc                                                                             >  1:24348/1‑93 (MQ=255)
              ttGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGt                                                                           >  2:1272130/1‑93 (MQ=255)
               tGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTa                                                                          <  1:2563796/93‑1 (MQ=255)
                 aTCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTAcc                                                                        >  2:3281590/1‑92 (MQ=255)
                 aTCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACg                                                                        <  2:4837912/93‑1 (MQ=255)
                 aTCAGTTCGCTGATATCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACg                                                                        >  2:3848821/1‑93 (MQ=255)
                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       <  1:2122522/93‑1 (MQ=255)
                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       <  2:424738/93‑1 (MQ=255)
                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       >  2:2160209/1‑93 (MQ=255)
                  tCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGc                                                                       >  1:6067748/1‑93 (MQ=255)
                   cAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCGAGGCACTCAAGACGACCGCGTACGCt                                                                      >  1:2573809/1‑93 (MQ=255)
                   cAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCt                                                                      <  2:2147326/93‑1 (MQ=255)
                   cAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCt                                                                      <  2:372239/93‑1 (MQ=255)
                    aGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGTCTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTg                                                                     >  1:3610783/1‑93 (MQ=255)
                     gTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGc                                                                    >  1:3984645/1‑93 (MQ=255)
                      ttCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGcc                                                                   >  2:2823636/1‑93 (MQ=255)
                      ttCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGcc                                                                   >  2:1383349/1‑93 (MQ=255)
                      ttCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGcc                                                                   <  1:1529323/93‑1 (MQ=255)
                       tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCa                                                                  <  2:6111585/93‑1 (MQ=255)
                       tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCa                                                                  <  2:4453860/93‑1 (MQ=255)
                       tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCa                                                                  <  2:5515546/93‑1 (MQ=255)
                         gCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGCCCCCTTAAAATAGCAAACCGCCTGCAAGGAAATCAAGACGCCGCCGTACGCGGCCAta                                                                >  2:2948806/1‑92 (MQ=255)
                         ccTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATc                                                                <  1:1541822/92‑1 (MQ=255)
                          cTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCa                                                               <  2:3218387/93‑1 (MQ=255)
                          cTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCa                                                               >  2:1489625/1‑93 (MQ=255)
                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              >  2:850131/1‑93 (MQ=255)
                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              >  2:4084574/1‑93 (MQ=255)
                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              >  2:1317457/1‑93 (MQ=255)
                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              <  1:3914382/93‑1 (MQ=255)
                           tGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCaa                                                              <  1:3427774/93‑1 (MQ=255)
                            gATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAt                                                             <  2:2764555/93‑1 (MQ=255)
                            gATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAAt                                                             >  2:5056599/1‑93 (MQ=255)
                              tttCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGa                                                           >  1:1869049/1‑93 (MQ=255)
                              tttCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGa                                                           <  1:1041672/93‑1 (MQ=255)
                                   gTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCata                                                      >  1:5939924/1‑93 (MQ=255)
                                   gTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCata                                                      >  1:9634/1‑93 (MQ=255)
                                    tGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCatat                                                     <  2:1960215/93‑1 (MQ=255)
                                      gAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCa                                                   <  2:1631428/93‑1 (MQ=255)
                                          tCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCg                                               <  2:6201612/93‑1 (MQ=255)
                                          tCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCg                                               >  2:5841118/1‑93 (MQ=255)
                                           cAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGc                                              <  2:173240/93‑1 (MQ=255)
                                             gTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTc                                            >  2:382974/1‑93 (MQ=255)
                                             gTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTc                                            <  1:2067438/93‑1 (MQ=255)
                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         >  2:5136352/1‑93 (MQ=255)
                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         <  1:533274/93‑1 (MQ=255)
                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         >  1:5882932/1‑93 (MQ=255)
                                                gCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGga                                         <  1:274351/93‑1 (MQ=255)
                                                 cATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgat                                        <  2:2114704/93‑1 (MQ=255)
                                                 cATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgat                                        <  2:5600642/93‑1 (MQ=255)
                                                   tGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgatga                                      <  1:328624/93‑1 (MQ=255)
                                                    gCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGgatgat                                     <  2:1897689/93‑1 (MQ=255)
                                                     cgccAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATa                                    <  1:6281942/91‑1 (MQ=255)
                                                      tCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATaa                                   >  1:2375367/1‑93 (MQ=255)
                                                       ccAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAc                                  <  2:5276580/93‑1 (MQ=255)
                                                       ccAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAAc                                  >  1:3441081/1‑93 (MQ=255)
                                                         aGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGc                                <  1:3088229/93‑1 (MQ=255)
                                                         aGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGc                                >  2:3286606/1‑93 (MQ=255)
                                                          gTCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGcc                               >  1:5834497/1‑93 (MQ=255)
                                                           tCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                              >  1:4054959/1‑93 (MQ=255)
                                                           tCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                              <  2:6040256/93‑1 (MQ=255)
                                                           tCCCCTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                              >  1:3940524/1‑93 (MQ=255)
                                                            ccccTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                             <  1:1783013/93‑1 (MQ=255)
                                                             cccTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGc                            >  2:4754829/1‑93 (MQ=255)
                                                               cTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCa                          >  2:6237890/1‑93 (MQ=255)
                                                               cTTAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATAAATGACCATATAACCAGCTCGGATGATAACGCCTGCCa                          >  1:1111870/1‑93 (MQ=255)
                                                                ttAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAt                         <  1:1658051/93‑1 (MQ=255)
                                                                ttAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAt                         <  1:1296494/93‑1 (MQ=255)
                                                                ttAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAt                         >  1:4227970/1‑93 (MQ=255)
                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        <  2:4607636/93‑1 (MQ=255)
                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        <  1:5485659/93‑1 (MQ=255)
                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        <  1:480219/93‑1 (MQ=255)
                                                                 tAAGACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCAtt                        >  1:4863192/1‑93 (MQ=255)
                                                                    gACTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACa                     <  2:5740972/93‑1 (MQ=255)
                                                                     aCTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAg                    <  1:2669190/93‑1 (MQ=255)
                                                                      cTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGa                   <  1:3238527/93‑1 (MQ=255)
                                                                      cTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGa                   <  2:1349787/93‑1 (MQ=255)
                                                                      cTGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGa                   <  2:5435682/93‑1 (MQ=255)
                                                                       tGCAAGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGAc                  >  1:4394193/1‑93 (MQ=255)
                                                                       cgCAAGACGTCGGCAAGCCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGAc                  <  2:93057/92‑1 (MQ=255)
                                                                          aaGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTa               <  2:2037552/93‑1 (MQ=255)
                                                                          aaGACGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTa               <  1:495109/93‑1 (MQ=255)
                                                                             aCGTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCg            <  1:5721155/93‑1 (MQ=255)
                                                                              cGTCTGCAAGGCACTCAAGCCGACCGCGTACGCTGCCTTCAATGCCCTTATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGa           <  1:6300943/93‑1 (MQ=255)
                                                                               gTCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAt          >  2:4034954/1‑93 (MQ=255)
                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         <  2:4727008/93‑1 (MQ=255)
                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  1:2854611/1‑93 (MQ=255)
                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  1:3766329/1‑93 (MQ=255)
                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  2:2340230/1‑93 (MQ=255)
                                                                                tCTGCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGAtt         >  1:5790550/1‑93 (MQ=255)
                                                                                   gCAAGGCACTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTg      >  2:5692501/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAAT  >  NC_000913/3917434‑3917613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑