BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,972,641	G→A	R145H (CGC→CAC)	wecD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,972,641	0	G	A	99.5%	775.4 / ‑5.4	214	R145H (CGC→CAC)	wecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (87/126); total (87/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

Only 100 of 219 total aligned reads displayed.

AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724

aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGt                                                                                 >  2:3670512/1‑93 (MQ=255)

 aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTa                                                                                <  2:1876845/93‑1 (MQ=255)

  tGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTaa                                                                               >  1:2636237/1‑93 (MQ=255)

   gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCATACGGCGCTAAACTGGGCGTATGCTCACGGTaaa                                                                              <  1:6100815/93‑1 (MQ=255)

   gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTaaa                                                                              <  1:3892424/93‑1 (MQ=255)

     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            >  1:5014049/1‑93 (MQ=255)

     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            >  2:4193222/1‑93 (MQ=255)

     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            >  1:3232726/1‑93 (MQ=255)

      aCAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaaca                                                                           >  2:8535/1‑93 (MQ=255)

      aCAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaaca                                                                           <  1:5741783/93‑1 (MQ=255)

         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        >  2:3077631/1‑93 (MQ=255)

         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        <  1:1804062/93‑1 (MQ=255)

           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  1:4119143/93‑1 (MQ=255)

           tGCGCGAATTGGCATGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  1:3601669/93‑1 (MQ=255)

            gcgcGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTg                                                                     <  2:3750722/93‑1 (MQ=255)

             cgcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGc                                                                    <  1:1607318/93‑1 (MQ=255)

              gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCg                                                                   >  1:76236/1‑93 (MQ=255)

               cgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCgg                                                                  <  1:5598442/93‑1 (MQ=255)

               cgAATTGACCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCgg                                                                  <  2:2075015/93‑1 (MQ=255)

                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGt                                                                <  1:4199707/93‑1 (MQ=255)

                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGt                                                                <  2:1543113/93‑1 (MQ=255)

                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTg                                                               <  2:745453/93‑1 (MQ=255)

                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTg                                                               >  2:5075796/1‑93 (MQ=255)

                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTg                                                               <  2:1118480/93‑1 (MQ=255)

                   ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTgg                                                              <  2:5317211/93‑1 (MQ=255)

                   ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTgg                                                              <  2:1361123/93‑1 (MQ=255)

                     ggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCg                                                            <  1:2133259/93‑1 (MQ=255)

                      gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGa                                                           <  2:897732/93‑1 (MQ=255)

                        ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAcc                                                         <  1:3304267/93‑1 (MQ=255)

                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGCAAAACAACTTTGCGGGTGGCGAccc                                                        >  2:5004806/1‑93 (MQ=255)

                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAccc                                                        <  1:661780/93‑1 (MQ=255)

                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:2340690/1‑93 (MQ=255)

                           ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa                                                      <  1:4819841/93‑1 (MQ=255)

                            tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  1:5673008/93‑1 (MQ=255)

                             ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    >  2:4396487/1‑93 (MQ=255)

                             ggctagACGCGGTGCAGGTGCTGAGCTGATGCAAACGTCGCTAAACTGTGCGTATGCTCACGGTAAAACACCTTTGCGGGTGGCGACCCAAAt                                                    <  1:1815227/93‑1 (MQ=255)

                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  1:2322984/93‑1 (MQ=255)

                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  2:1064995/93‑1 (MQ=255)

                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  1:5705840/93‑1 (MQ=255)

                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   >  1:2707608/1‑93 (MQ=255)

                               ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  1:6243738/93‑1 (MQ=255)

                               ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  1:4483015/93‑1 (MQ=255)

                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 <  2:1427926/93‑1 (MQ=255)

                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 >  1:3524514/1‑93 (MQ=255)

                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 >  2:3708717/1‑93 (MQ=255)

                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                >  1:3487763/1‑93 (MQ=255)

                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:552637/93‑1 (MQ=255)

                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:3242446/93‑1 (MQ=255)

                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:5837926/93‑1 (MQ=255)

                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                >  1:4555949/1‑93 (MQ=255)

                                  gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa                                               >  2:2594470/1‑93 (MQ=255)

                                  gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa                                               >  2:4038728/1‑93 (MQ=255)

                                   aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              <  1:6128453/93‑1 (MQ=255)

                                     gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca                                            <  2:1689923/93‑1 (MQ=255)

                                      cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           >  1:5334523/1‑93 (MQ=255)

                                      cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           <  2:3833332/93‑1 (MQ=255)

                                         tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        <  1:3086773/93‑1 (MQ=255)

                                           cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgccg                                      >  2:3536467/1‑93 (MQ=255)

                                            aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc                                     <  2:3933688/93‑1 (MQ=255)

                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   <  1:1263472/93‑1 (MQ=255)

                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   <  2:2504680/93‑1 (MQ=255)

                                              gTGCTGAGCTGATGCAAACCGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   >  2:6140264/1‑93 (MQ=255)

                                                   gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              <  1:3009863/93‑1 (MQ=255)

                                                    agctgaTGCAGACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  2:3312131/93‑1 (MQ=255)

                                                    agctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  1:5829370/93‑1 (MQ=255)

                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  2:1191622/1‑93 (MQ=255)

                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  1:2792232/1‑93 (MQ=255)

                                                       tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          >  2:5683521/1‑93 (MQ=255)

                                                       tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          >  2:684299/1‑93 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  2:4696453/1‑93 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:1337130/1‑93 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  2:1028328/1‑93 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:5880573/1‑93 (MQ=255)

                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        <  1:1073264/93‑1 (MQ=255)

                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGAGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        <  1:3389431/93‑1 (MQ=255)

                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  2:2799147/93‑1 (MQ=255)

                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       >  2:4540461/1‑93 (MQ=255)

                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  1:5856793/93‑1 (MQ=255)

                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  1:5116631/93‑1 (MQ=255)

                                                            cAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata                     >  1:1483664/1‑93 (MQ=255)

                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   <  2:5726226/93‑1 (MQ=255)

                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   >  1:1909740/1‑93 (MQ=255)

                                                               aCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  <  1:1690247/93‑1 (MQ=255)

                                                                 ggCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAg                >  2:3476136/1‑93 (MQ=255)

                                                                    gcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:3117887/1‑93 (MQ=255)

                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:5909557/93‑1 (MQ=255)

                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  1:5243546/93‑1 (MQ=255)

                                                                      tAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAGACGATACATACAAAgtggtg           >  1:4203993/1‑93 (MQ=255)

                                                                      tAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           <  2:1196511/93‑1 (MQ=255)

                                                                       aaaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          >  2:2447085/1‑93 (MQ=255)

                                                                       aaaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          <  2:2284423/93‑1 (MQ=255)

                                                                        aaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCg         >  1:1470502/1‑93 (MQ=255)

                                                                         aCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGa        <  2:6290791/93‑1 (MQ=255)

                                                                           tgtgCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  1:465365/90‑1 (MQ=255)

                                                                           tGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  2:4279176/93‑1 (MQ=255)

                                                                            gggCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     <  2:3160017/93‑1 (MQ=255)

                                                                              gCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:3109213/93‑1 (MQ=255)

                                                                              gCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:435271/93‑1 (MQ=255)

                                                                              gCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:111028/93‑1 (MQ=255)

                                                                               cGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAg  <  1:3718171/93‑1 (MQ=255)

AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`