BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,041	A→G	S863P (TCC→CCC)	acrB ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,041	0	A	G	100.0%	693.5 / NA	215	S863P (TCC→CCC)	acrB	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/107); total (108/107)

Only 100 of 215 total aligned reads displayed.

CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCG  >  NC_000913/480951‑481130

cGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCa                                                                                         <  1:6160454/93‑1 (MQ=255)

cGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCa                                                                                         >  2:5670628/1‑93 (MQ=255)

 gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCAt                                                                                        >  1:3761647/1‑93 (MQ=255)

     aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                    <  1:719669/93‑1 (MQ=255)

     aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                    <  2:3776077/93‑1 (MQ=255)

      gcgcCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCg                                                                                   >  1:5651527/1‑93 (MQ=255)

         ccgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTcc                                                                                <  1:3644382/93‑1 (MQ=255)

         ccgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTcc                                                                                <  1:4413965/93‑1 (MQ=255)

          cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCa                                                                               >  2:6379/1‑93 (MQ=255)

           gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAg                                                                              >  1:491862/1‑93 (MQ=255)

            ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGt                                                                             >  2:426770/1‑93 (MQ=255)

             cAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCGCGTCCAGTc                                                                            >  1:5536217/1‑93 (MQ=255)

               gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                          >  1:2756452/1‑93 (MQ=255)

                 cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAg                                                                        >  2:1513048/1‑93 (MQ=255)

                  acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGc                                                                       <  2:4763993/93‑1 (MQ=255)

                     ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                    >  1:1605274/1‑93 (MQ=255)

                      gAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAac                                                                   <  1:1781631/93‑1 (MQ=255)

                       aaCACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAaca                                                                  <  2:904098/93‑1 (MQ=255)

                       aaCACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAaca                                                                  <  1:1339354/93‑1 (MQ=255)

                        acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAacac                                                                 <  2:1628967/93‑1 (MQ=255)

                        acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAacac                                                                 <  1:527286/93‑1 (MQ=255)

                         cacGACAATCAGCGAAATCGCGTACAGTGAAGGTGCCTAGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                                <  2:328548/93‑1 (MQ=39)

                         cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                                <  2:1223115/93‑1 (MQ=255)

                         cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                                >  2:279858/1‑93 (MQ=255)

                         cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                                >  2:4466541/1‑93 (MQ=255)

                            gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGt                                                             >  2:1358774/1‑93 (MQ=255)

                            gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGt                                                             >  1:4491661/1‑93 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                            <  1:3371984/93‑1 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                            <  1:5435233/93‑1 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                            <  1:460170/93‑1 (MQ=255)

                              cAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGTCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                           <  2:3167574/93‑1 (MQ=255)

                              cAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                           <  1:3792829/93‑1 (MQ=255)

                               aaTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                          <  2:4090887/93‑1 (MQ=255)

                                aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                         >  2:2935959/1‑93 (MQ=255)

                                 tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCa                                                        <  2:239273/93‑1 (MQ=255)

                                   aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                      >  2:3449206/1‑93 (MQ=255)

                                   aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                      <  2:1301676/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                     <  1:3285577/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                     >  2:2492711/1‑93 (MQ=255)

                                     cGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGttt                                                    >  2:2839585/1‑93 (MQ=255)

                                     cGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGttt                                                    >  2:779602/1‑93 (MQ=255)

                                      gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                   >  1:2444020/1‑93 (MQ=255)

                                       aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                  >  2:2792958/1‑93 (MQ=255)

                                         aTCGCGTACAGTGAAGGGGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGGCATAGCCAACACCGGTGGGCAGTTTGCtg                                                >  1:3903769/1‑92 (MQ=255)

                                                aCAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCAGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtt                                         >  2:4651982/1‑93 (MQ=255)

                                                 cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttg                                        >  1:4272822/1‑93 (MQ=255)

                                                 cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttg                                        <  2:399758/93‑1 (MQ=255)

                                                 cAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtcg                                        >  1:2992230/1‑91 (MQ=255)

                                                  aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                       <  1:5482550/93‑1 (MQ=255)

                                                   gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                      >  2:591378/1‑93 (MQ=255)

                                                   gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                      <  1:1928206/93‑1 (MQ=255)

                                                   gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                      <  2:3578339/93‑1 (MQ=255)

                                                    tGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTc                                     <  2:1916242/93‑1 (MQ=255)

                                                     gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcc                                    <  2:3851760/93‑1 (MQ=255)

                                                     gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcc                                    >  1:1202898/1‑93 (MQ=255)

                                                      aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                   <  1:1179274/93‑1 (MQ=255)

                                                        ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 >  1:808043/1‑93 (MQ=255)

                                                        ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 >  1:3978499/1‑93 (MQ=255)

                                                        ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 <  1:4039266/93‑1 (MQ=255)

                                                        ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 <  2:6117804/93‑1 (MQ=255)

                                                         gTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCa                                <  2:4104357/93‑1 (MQ=255)

                                                           gCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGc                              >  2:1980213/1‑93 (MQ=255)

                                                             cTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                            >  2:3943407/1‑93 (MQ=255)

                                                             cTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                            <  1:2322154/93‑1 (MQ=255)

                                                             cTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                            >  1:556252/1‑93 (MQ=255)

                                                              tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                           >  1:4404341/1‑93 (MQ=255)

                                                              tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                           >  1:4674025/1‑93 (MQ=255)

                                                              tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                           >  1:5088356/1‑93 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                         <  1:3242419/93‑1 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                         >  2:3836212/1‑93 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                         <  1:5940022/93‑1 (MQ=255)

                                                                 ttGCGGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                        <  2:1248741/93‑1 (MQ=255)

                                                                  tGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTg                       <  2:3952484/93‑1 (MQ=255)

                                                                   gCCGGAGATACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                      >  2:6160268/1‑93 (MQ=255)

                                                                   gCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                      >  1:5069504/1‑93 (MQ=255)

                                                                   gCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                      <  2:4944241/93‑1 (MQ=255)

                                                                    ccGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                     >  2:2628413/1‑93 (MQ=255)

                                                                      ggAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                   >  2:2021854/1‑93 (MQ=255)

                                                                      ggAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                   >  2:1025511/1‑93 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                  <  1:157332/93‑1 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                  >  1:4190644/1‑93 (MQ=255)

                                                                        agagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                 <  2:4158609/93‑1 (MQ=255)

                                                                         gagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAcc                >  2:5449905/1‑93 (MQ=255)

                                                                            aCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGt             <  2:5130081/93‑1 (MQ=255)

                                                                            aCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGt             <  1:1263970/93‑1 (MQ=255)

                                                                              gTTCCTGATAGGGCATCCCCGTCTAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc           >  1:2913180/1‑93 (MQ=255)

                                                                              gTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc           <  2:2347095/93‑1 (MQ=255)

                                                                               ttCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt          <  1:4363641/93‑1 (MQ=255)

                                                                                tCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtt         <  1:5044678/93‑1 (MQ=255)

                                                                                 ccTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACttt        >  2:1186347/1‑93 (MQ=255)

                                                                                  cTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtttt       >  1:533746/1‑93 (MQ=255)

                                                                                  cTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtttt       <  1:5984372/93‑1 (MQ=255)

                                                                                   tGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTa      >  2:2665375/1‑93 (MQ=255)

                                                                                   tGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTa      >  2:2201501/1‑93 (MQ=255)

                                                                                    gATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc     <  1:3493238/93‑1 (MQ=255)

                                                                                     aTAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc    >  1:3718205/1‑93 (MQ=255)

                                                                                      tAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc   <  1:5830167/93‑1 (MQ=255)

                                                                                      tAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc   <  1:1494673/93‑1 (MQ=255)

                                                                                       aGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCg  >  2:2381012/1‑93 (MQ=255)

                                                                                       aGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCg  <  1:6022108/93‑1 (MQ=255)

CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCG  >  NC_000913/480951‑481130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`