BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,390	C→T	A65V (GCG→GTG)	hemG →	protoporphyrin oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,390	0	C	T	100.0%	902.1 / NA	240	A65V (GCG→GTG)	hemG	protoporphyrin oxidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (124/116); total (124/116)

Only 100 of 246 total aligned reads displayed.

CCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGG  >  NC_000913/4032300‑4032479

 cAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGtt                                                                                        >  1:4012019/1‑93 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                       >  1:724692/1‑93 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                       >  1:633491/1‑93 (MQ=255)

  aaTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTc                                                                                       <  1:82023/93‑1 (MQ=255)

   aTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTcc                                                                                      >  2:3779473/1‑93 (MQ=255)

   aTGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTcc                                                                                      >  2:5980378/1‑93 (MQ=255)

     gtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAg                                                                                    >  1:1142720/1‑93 (MQ=255)

       gCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGa                                                                                  <  1:206371/93‑1 (MQ=255)

        cACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGaa                                                                                 <  2:2938700/93‑1 (MQ=255)

         aCCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAt                                                                                <  1:2152260/93‑1 (MQ=255)

         aCCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAt                                                                                >  1:6159837/1‑93 (MQ=255)

         aCCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAt                                                                                >  2:4229558/1‑93 (MQ=255)

           cGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAttt                                                                              >  1:4689132/1‑93 (MQ=255)

            gCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTg                                                                             >  1:1361528/1‑93 (MQ=255)

            gCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTg                                                                             >  1:6195518/1‑93 (MQ=255)

            gCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTg                                                                             <  2:3531373/93‑1 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                          >  2:3210624/1‑93 (MQ=255)

               ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa                                                                          >  1:2880909/1‑93 (MQ=255)

                 gaagaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaa                                                                        >  1:1577528/1‑93 (MQ=255)

                  aagaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaa                                                                       >  1:5282461/1‑93 (MQ=255)

                   agaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaaa                                                                      <  2:4714705/93‑1 (MQ=255)

                    gaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaaaa                                                                     <  2:3110727/93‑1 (MQ=255)

                     aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTTCCATTCAGTGTTCCAGGAATTTGTCAAAAAAc                                                                    <  1:608895/93‑1 (MQ=255)

                     aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAc                                                                    <  2:4041270/93‑1 (MQ=255)

                      aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa                                                                   <  1:4864396/93‑1 (MQ=255)

                       ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt                                                                  <  2:366172/93‑1 (MQ=255)

                        cacgGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATg                                                                 <  1:3449765/93‑1 (MQ=255)

                        cacgGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATg                                                                 >  1:1998480/1‑93 (MQ=255)

                          cggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCg                                                               <  1:3656039/91‑1 (MQ=255)

                           ggTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGa                                                              <  2:2128317/92‑1 (MQ=255)

                            gTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAc                                                             <  1:762798/93‑1 (MQ=255)

                            gTGAGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAc                                                             <  1:4572455/93‑1 (MQ=255)

                             tGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcg                                                            <  1:22233/93‑1 (MQ=255)

                              gggAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAcgc                                                           <  1:4488424/93‑1 (MQ=255)

                                 aaaaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGc                                                        <  2:4398901/93‑1 (MQ=255)

                                 aaaaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGc                                                        <  2:795524/93‑1 (MQ=255)

                                  aaaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCt                                                       <  1:1519166/93‑1 (MQ=255)

                                  aaaCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCt                                                       <  1:5828162/93‑1 (MQ=255)

                                    aCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGa                                                     >  1:3298176/1‑93 (MQ=255)

                                    aCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGa                                                     >  1:3365377/1‑93 (MQ=255)

                                     cTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGaa                                                    <  2:5935210/93‑1 (MQ=255)

                                     cTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGaa                                                    >  1:4061513/1‑93 (MQ=255)

                                     cTATGACCGGGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGGTGaa                                                    >  2:4523405/1‑93 (MQ=255)

                                      tATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAt                                                   >  1:3852618/1‑93 (MQ=255)

                                       aTGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAtt                                                  <  1:1418640/93‑1 (MQ=255)

                                       aTGACCGTGTGGTCATTGGGGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAAtt                                                  <  2:5664875/93‑1 (MQ=255)

                                        tGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTc                                                 <  1:3773913/93‑1 (MQ=255)

                                         gACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCg                                                >  2:5449238/1‑93 (MQ=255)

                                         gACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCg                                                >  1:862695/1‑93 (MQ=255)

                                          aCCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGa                                               >  2:2679935/1‑93 (MQ=255)

                                            cGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATg                                             >  1:1947096/1‑93 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                            <  2:3460114/93‑1 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                            >  1:2303278/1‑93 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                            <  1:5320043/93‑1 (MQ=255)

                                             gtgtGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGc                                            <  2:1261749/93‑1 (MQ=255)

                                              tgtgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGcc                                           <  2:5271116/93‑1 (MQ=255)

                                              tgtgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGcc                                           <  1:488406/93‑1 (MQ=255)

                                               gtgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCg                                          <  2:5809976/93‑1 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                         >  1:1677663/1‑93 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                         <  1:5708879/93‑1 (MQ=255)

                                                tgGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGa                                         >  1:4369300/1‑93 (MQ=255)

                                                     aTTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGcc                                    >  2:24576/1‑93 (MQ=255)

                                                     aTTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGcc                                    >  1:4972732/1‑93 (MQ=255)

                                                     aTTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGcc                                    >  2:5160822/1‑93 (MQ=255)

                                                      ttGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCt                                   >  2:4825847/1‑93 (MQ=255)

                                                       tGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCtt                                  <  1:3039474/93‑1 (MQ=255)

                                                         gTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTAGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCtttt                                <  1:3714298/93‑1 (MQ=255)

                                                           gCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTAc                              >  2:3185738/1‑93 (MQ=255)

                                                             ttCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTc                            >  2:4366496/1‑93 (MQ=255)

                                                             ttCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTc                            <  1:1278821/93‑1 (MQ=255)

                                                             ttCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTc                            <  1:1631103/93‑1 (MQ=255)

                                                             ttCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTc                            <  1:179459/93‑1 (MQ=255)

                                                              tCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTcc                           >  2:1582029/1‑93 (MQ=255)

                                                               cTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCg                          <  2:1754732/93‑1 (MQ=255)

                                                                tATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGt                         >  2:4328411/1‑93 (MQ=255)

                                                                   tCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGaa                      >  1:2716650/1‑93 (MQ=255)

                                                                   tCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGaa                      >  1:6064995/1‑93 (MQ=255)

                                                                    tgCTATGGTCACTACCATTCAGTGTTCCAAGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAAt                     <  1:1885634/92‑1 (MQ=255)

                                                                     gCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATc                    >  2:4667012/1‑93 (MQ=255)

                                                                       tATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtg                  <  1:5057964/93‑1 (MQ=255)

                                                                        aTGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtgg                 >  2:2965664/1‑93 (MQ=255)

                                                                        aTGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtgg                 <  2:5841965/93‑1 (MQ=255)

                                                                         tGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggt                <  2:2051752/93‑1 (MQ=255)

                                                                           gTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggtgg              <  2:1364513/93‑1 (MQ=255)

                                                                           gTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCtggtgg              <  1:2698548/93‑1 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGCGCGCCTTTTACTCCGTGCATCTGGTGgcgc           >  2:5291123/1‑93 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc           >  2:1102022/1‑93 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAACGCCTTTTACTCCGTGAATCTGGTGgcgc           <  1:2541558/93‑1 (MQ=255)

                                                                              aCTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGACGAGCGCCTTTTACTCCGTGAATCTGGTGgcgc           >  2:2751826/1‑93 (MQ=255)

                                                                                tACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGGATCTGGTGgcgcgc         >  2:2229796/1‑93 (MQ=255)

                                                                                tACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcgc         >  2:5064109/1‑93 (MQ=255)

                                                                                tACCATTCAGTGTTCCAGGAATTTGACAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGgcgcgc         >  1:4960249/1‑93 (MQ=255)

                                                                                 aCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa        >  2:950793/1‑93 (MQ=255)

                                                                                 aCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCa        >  1:988048/1‑93 (MQ=255)

                                                                                  ccATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaa       <  2:391593/93‑1 (MQ=255)

                                                                                  ccATTCAGTGTTCCAGGAACTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaa       >  2:6125873/1‑93 (MQ=255)

                                                                                   cATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaaa      >  2:2519064/1‑93 (MQ=255)

                                                                                   cATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCaaa      >  1:3081936/1‑93 (MQ=255)

                                                                                    aTTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAc     >  2:5497785/1‑93 (MQ=255)

                                                                                     ttCAGTGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAAcc    <  2:3012653/93‑1 (MQ=255)

CCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGACGCGGCTGAATTCGATGCCGAGCGCCTTTTACTCCGTGAATCTGGTGGCGCGCAAACCGG  >  NC_000913/4032300‑4032479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`