BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,182	T→C	I280T (ATC→ACC)	htpG →	chaperone Hsp90

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,182	0	T	C	99.6%	780.4 / NA	241	I280T (ATC→ACC)	htpG	chaperone Hsp90
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (126/114); minor base A (1/0); total (127/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

ACAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGACGACG  >  NC_000913/495092‑495271

aCAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAccc                                                                                         <  1:1876168/93‑1 (MQ=255)

 cAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAcccc                                                                                        <  1:3494753/93‑1 (MQ=255)

 cAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAcccc                                                                                        <  1:5985559/93‑1 (MQ=255)

   aaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGt                                                                                      >  2:2550604/1‑93 (MQ=255)

    acacATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTc                                                                                     >  2:4391387/1‑93 (MQ=255)

     cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTcc                                                                                    >  1:5653756/1‑93 (MQ=255)

     cacaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTcc                                                                                    <  2:53470/93‑1 (MQ=255)

      acaTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTccc                                                                                   >  1:69133/1‑93 (MQ=255)

       caTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCa                                                                                  <  1:5695639/93‑1 (MQ=255)

       caTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACACCGTCCCa                                                                                  >  1:95975/1‑93 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 >  2:5618705/1‑93 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 <  2:1001330/93‑1 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 >  1:1420794/1‑93 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 <  2:1650359/93‑1 (MQ=255)

        aTCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAg                                                                                 <  1:1070375/93‑1 (MQ=255)

         tCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGGACACCAGCCTGCTGTACACCCCGTCCCAgg                                                                                >  2:3444268/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  2:4285255/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  2:1557119/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  1:4183722/1‑93 (MQ=255)

           gCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGGAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCt                                                                              >  2:4722866/1‑93 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            <  2:5575207/93‑1 (MQ=255)

             ccACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTcc                                                                            <  2:5721221/93‑1 (MQ=255)

              cACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCg                                                                           >  2:4695958/1‑93 (MQ=255)

                  aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTggg                                                                       <  2:2208344/93‑1 (MQ=255)

                  aCTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTggg                                                                       >  1:6230030/1‑93 (MQ=255)

                    tttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGat                                                                     <  2:14798/93‑1 (MQ=255)

                     ttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTTCTGTACACCCCGTCCCAGGCTCCGTGGGata                                                                    <  1:6112005/93‑1 (MQ=255)

                     ttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGata                                                                    <  1:595109/93‑1 (MQ=255)

                     ttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGata                                                                    <  1:3015308/93‑1 (MQ=255)

                     ttAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGata                                                                    >  1:4518426/1‑93 (MQ=255)

                          gATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTgg                                                               >  2:5862841/1‑93 (MQ=255)

                             cggCTGACCTGGAGCCACAACCGGGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAAc                                                            <  1:16102/91‑1 (MQ=255)

                              cGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAAcc                                                           <  1:31073/93‑1 (MQ=255)

                               gCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcg                                                          >  1:2828754/1‑93 (MQ=255)

                                cTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTTCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgc                                                         <  1:5106350/93‑1 (MQ=255)

                                cTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgc                                                         >  1:4866355/1‑93 (MQ=255)

                                 tGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgcg                                                        >  2:2090379/1‑93 (MQ=255)

                                   cccTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGAt                                                      <  1:1741545/92‑1 (MQ=255)

                                   aCCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGAt                                                      <  2:4528264/93‑1 (MQ=255)

                                     cTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCa                                                    >  2:3681423/1‑93 (MQ=255)

                                     cTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCa                                                    >  1:2107957/1‑93 (MQ=255)

                                     cTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCa                                                    <  1:5848154/93‑1 (MQ=255)

                                      tGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCAt                                                   >  2:2145801/1‑93 (MQ=255)

                                       ggAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa                                                  >  1:5583591/1‑93 (MQ=255)

                                        gAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATaa                                                 <  2:5227232/93‑1 (MQ=255)

                                         aGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATaaa                                                <  2:4860238/93‑1 (MQ=255)

                                         aGCCACAACCGTGTTGAAGGGAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATaaa                                                >  2:6246391/1‑93 (MQ=255)

                                          gCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAac                                               <  1:5502202/93‑1 (MQ=255)

                                          gCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAac                                               <  1:4568851/93‑1 (MQ=255)

                                           ccACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAaca                                              <  1:767348/93‑1 (MQ=255)

                                           ccACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAaca                                              <  2:3996434/93‑1 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             >  1:3921377/1‑93 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             >  1:3263873/1‑93 (MQ=255)

                                            cacaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACAACCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAacac                                             >  2:5389198/1‑93 (MQ=255)

                                               aaCCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACCCCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGc                                          <  2:4967961/93‑1 (MQ=255)

                                               aaCCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGc                                          >  1:2574851/1‑93 (MQ=255)

                                               aaCCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGc                                          <  1:4015049/93‑1 (MQ=255)

                                                   gtgtTGAAGGTAAGCAGGAGTACACCAGCCTTCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGa                                      <  1:2137216/93‑1 (MQ=255)

                                                   gtgtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGa                                      <  2:6275632/93‑1 (MQ=255)

                                                   gtgtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGa                                      >  1:3768614/1‑93 (MQ=255)

                                                    tgtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGaa                                     >  1:306878/1‑93 (MQ=255)

                                                     gtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGaac                                    >  1:1105186/1‑92 (MQ=255)

                                                     gtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGaaa                                    >  1:2287908/1‑93 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   >  2:3274333/1‑93 (MQ=255)

                                                      ttGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAAc                                   >  2:1260358/1‑93 (MQ=255)

                                                        gAAGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTg                                 <  2:5184352/93‑1 (MQ=255)

                                                         aaGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGt                                <  2:2323231/93‑1 (MQ=255)

                                                         aaGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGt                                >  2:3036934/1‑93 (MQ=255)

                                                          aGGTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTa                               <  2:1212498/93‑1 (MQ=255)

                                                           ggTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGACACTGTAt                              >  2:4083940/1‑93 (MQ=255)

                                                           ggTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTAt                              >  1:1494777/1‑93 (MQ=255)

                                                           ggTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTAt                              <  1:3362324/93‑1 (MQ=255)

                                                           ggTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTAt                              <  2:6070999/93‑1 (MQ=255)

                                                           ggTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTAt                              <  2:6098698/93‑1 (MQ=255)

                                                            gTAAGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATg                             <  2:206111/93‑1 (MQ=255)

                                                              aaGCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATAATAAACACGGCCTGAAACTGTATGtt                           >  1:1819032/1‑93 (MQ=255)

                                                               aGCAGGAGTACACCAGCCGTCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTc                          <  2:482161/93‑1 (MQ=255)

                                                                gCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCa                         <  1:1358996/93‑1 (MQ=255)

                                                                gCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCa                         >  2:1797152/1‑93 (MQ=255)

                                                                gCAGGAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCa                         <  1:4428528/93‑1 (MQ=255)

                                                                   ggAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCg                      <  2:2464737/93‑1 (MQ=255)

                                                                    gAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgt                     <  2:868226/93‑1 (MQ=255)

                                                                    gAGTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgt                     >  2:4093675/1‑93 (MQ=255)

                                                                      gTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgt                   <  1:5381183/93‑1 (MQ=255)

                                                                      gTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgt                   >  2:4504837/1‑93 (MQ=255)

                                                                      gTACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgt                   >  1:2651143/1‑93 (MQ=255)

                                                                       tACACCAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCgtgtg                  >  1:2944605/1‑93 (MQ=255)

                                                                         cacCAGCCTGCTGTACACCCCGTCCCAGGCTCTGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGtt                <  1:1172907/93‑1 (MQ=255)

                                                                           ccAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtca              >  2:2237246/1‑93 (MQ=255)

                                                                           ccAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtca              <  2:2650828/93‑1 (MQ=255)

                                                                            cAGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcat             >  2:4215089/1‑93 (MQ=255)

                                                                             aGCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatc            >  1:1239039/1‑93 (MQ=255)

                                                                              gCCTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTtcatca           >  2:3846990/1‑93 (MQ=255)

                                                                               ccTGCTGTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTcatcat          <  1:4644086/93‑1 (MQ=255)

                                                                                ctgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATg         <  1:2351789/93‑1 (MQ=255)

                                                                                 tgctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATgg        >  1:909561/1‑93 (MQ=255)

                                                                                  tctgTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGga       <  1:4258305/92‑1 (MQ=255)

                                                                                     gTACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGgacga    <  2:3616899/93‑1 (MQ=255)

                                                                                      tACACCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGgacgcc   >  2:1497234/1‑91 (MQ=255)

                                                                                       acacCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGacgacg  >  2:5450393/1‑93 (MQ=255)

ACAAACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAACACGGCCTGAAACTGTATGTTCAGCGTGTGTTCATCATGGACGACG  >  NC_000913/495092‑495271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`